The path of canine visceral leishmaniasis versus the path of Center for Zoonoses Control: contributions of spatial analysis to health

Matsumoto, Patrícia Sayuri Silvestre; Flores, Edilson Ferreira; Barbosa, José Seguinot; Pessoto, Umberto Catarino; Tolezano, José Eduardo; Hiramoto, Roberto Mitsuyoshi; Taniguchi, Helena Hilomi; Guimarães, Raúl Borges

doi:10.1590/0102-311x00272020

Cited by 2 publications

References 34 publications

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Comparison of cytotoxicity of Miltefosine and its niosomal form on chick embryo model

Seyedi,

Sharifi,

Khosravi

et al. 2024

Sci Rep

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Various drugs have been used for the treatment of leishmaniasis, but they often have adverse effects on the body's organs. In this study, we aimed to explore the effects of one type of drug, Miltefosine (MIL), and its analogue or modifier, liposomal Miltefosine (NMIL), on several fetal organs using both in silico analysis and practical tests on chicken embryos. Our in silico approach involved predicting the affinities of MIL and NMIL to critical proteins involved in leishmaniasis, including Vascular Endothelial Growth Factor A (VEGF-A), the Kinase insert domain receptor (KDR1), and apoptotic-regulator proteins (Bcl-2-associate). We then validated and supported these predictions through in vivo investigations, analyzing gene expression and pathological changes in angiogenesis and apoptotic mediators in MIL- and NMIL-treated chicken embryos. The results showed that NMIL had a more effective action towards VEGF-A and KDR1 in leishmaniasis, making it a better candidate for potential operative treatment during pregnancy than MIL alone. In vivo, studies also showed that chicken embryos under MIL treatment displayed less vascular mass and more degenerative and apoptotic changes than those treated with NMIL. These results suggest that NMIL could be a better treatment option for leishmaniasis during pregnancy.

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Comparison of cytotoxicity of Miltefosine and its niosomal form on chick embryo model

Seyedi,

Sharifi,

Khosravi

et al. 2024

Sci Rep

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Impact of the household environment risk for maintenance of natural foci ofLeishmania infantumtransmission to human and animal hosts in endemic areas for visceral leishmaniasis in Sao Paulo State, Brazil

Pss

Vbr

et al. 2021

Preprint

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When it comes to visceral leishmaniasis (VL) in Brazil, one of the main targets of public health policies of surveillance is the control of domestic canine reservoirs of Leishmania infantum. This paper aims to evaluate the effect of the household environment risk in the maintenance of natural foci and in the transmission to human and animal hosts in an endemic city for VL, Bauru, in Brazil. We collected 6,578 blood samples of dogs living in 3,916 households from Nov.2019 to Mar.2020 and applied geospatial models to predict the disease risk based on the canine population. We used Kernel density estimation, cluster analysis, geostatistics and Generalized Additive Models (GAM). To validate our models, we used cross-validation and created a ROC graph. We found an overall canine VL (CVL) prevalence of 5.6%. Odds ratios (OR) for CVL increased progressively according to the number of canines for >2 dogs (OR 2.70); households that already had CVL in the past increased the chances for CVL currently (OR 2.73); and the cases of CVL increase the chances for human VL cases (OR 1.16). Our models were statistically significant and demonstrated an association between the canine and human disease, mainly in VL foci that remain endemic. Although the Kernel ratio map had the best performance (AUC=82), all the models showed high risk in the city's northwest area. Canine population dynamics must be considered in public policies and geospatial methods may help target priority areas and planning VL surveillance in low and middle-income countries.

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The path of canine visceral leishmaniasis versus the path of Center for Zoonoses Control: contributions of spatial analysis to health

Cited by 2 publications

References 34 publications

Comparison of cytotoxicity of Miltefosine and its niosomal form on chick embryo model

Comparison of cytotoxicity of Miltefosine and its niosomal form on chick embryo model

Impact of the household environment risk for maintenance of natural foci ofLeishmania infantumtransmission to human and animal hosts in endemic areas for visceral leishmaniasis in Sao Paulo State, Brazil

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