Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata

Abstract

Aim: to evaluate the presence of subclinical HPV-induced anal lesions with anal cytology, High-Resolution Anoscopy (HRA) and HPV genotyping by polymerase chain reaction (PCR) in the follow-up of treated condylomata acuminata (CA). Methods: seventy-nine male patients were included. One month after anal CA eradication, the patients underwent brush samples collection for anal cytology and PCR, and HRA with biopsy of acetowhite lesions. These methods were compared within all patients and between groups, according to Human Immunodeficiency Virus (HIV) infection status: HIV-negative; HIV-positive with TCD4 count above and below 350 cells/mm3. Results: the most frequent HPV types were 6 and 16. HPV DNA was isolated in 92%. HIV infection was associated with a higher number of oncogenic HPV types (p=0.038). All patients with negative PCR had negative HRA and cytology. There were no differences in cytological, HRA or histopathological findings between groups. Conclusion: the association of the findings of cytopathology, HRA and genotyping of HPV refined the diagnosis of HPV-induced lesions. The degree of immunodeficiency was not associated with increase in remnant HPV-induced anal lesions.

Consequências imunológicas e sintomatológicas decorrentes da infecção do Vírus da Imunodeficiência Humana (HIV)

“…Tendo dados referentes a doenças sexualmente transmissíveis, Nadal et al (9) , em seu estudo sobre comparação citológica do HPV em pacientes com HIV. A associação dos achados citopatológico, colposcópico e PCR melhorou a acurácia do diagnóstico de lesões anais HPVinduzidas.…”

Conhecimento de acadêmicos de enfermagem sobre a vacina contra o papilomavírus humano

“…(5) Dentre os tipos de baixo risco, os 6 e 11 são os mais detectados em lesões de condiloma acuminado. (6) Sua principal estratégia de rastreamento é a realização periódica do exame citopatológico, que mostra resultados reconhecidos pelo Instituto Nacional de Câncer (INCA), identificando até 90% dos casos, mesmo antes da manifestação de sintomas, com diminuição da mortalidade em até 50%. (7) O exame é preconizado para mulheres entre 25 e 64 anos que já iniciaram atividade sexual, sendo recomendada sua realização uma vez por ano e, após dois exames anuais consecutivos negativos, a cada três anos.…”

HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum

“… 3 HPV infection has three manifestations: clinical infection (typical CA), subclinical human papillomavirus infection (SPI) and latent human papillomavirus infection (LPI). 4 SPI is an indicator of unobvious skin lesions seen by naked eyes while the acetic acid white test is positive 5 . The pathological manifestations are squamous epithelial atypical hyperplasia, visible or suspicious hollow cells and the HPV test is positive.…”