Avaliação da eficácia de três marcadores imunoistoquímicos envolvidos nos diferentes tempos da cicatrização da ferida cirúrgica

Filho, Octávio Antonio Azevedo da Costa; Filho, Jurandir Marcondes Ribas; Ariede, Bruno Luiz; Cavalcanti, T. P; Scapini, João Guilherme Seifert; Pasetto, Camila Vitola

doi:10.1590/0100-69912017004011

Cited by 1 publication

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References 26 publications

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“…Afterwards, the slides were scanned on the Axio Scan.Z1 scanner (Carl Zeiss®, Jena, Germany) and analyzed using Image Pro Plus 4 software (Media Cybernetics Inc., Rockville, USA). The percentage area immunolabeled with COX-2, in µm² , was calculated by evaluating 7 images for each replicate according to the methodology of Costa Filho et al [10].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Simultaneous feeding of calcium butyrate and tannin extract decreased the incidence of diarrhea and proinflammatory markers in weaned piglets

et al. 2022

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Objective: To investigate the effect of associating calcium butyrate with tannin extract, compared to an antimicrobial on the growth performance, incidence of diarrhea, intestinal histology, immune-expression of COX-2 and TNF-α of piglets.Methods: Seventy-two piglets (36 barrows and 36 gilts) weaned at 28±2 d and initial body weight of 7.17±1.07 kg were allocated to 3 treatments in a randomized complete block design with 8 replicates per treatment and 3 animals per experimental unit. Treatments were composed of NC -negative control: basal diet without additives; PC -positive control: basal diet + 40 mg/kg of colistin sulfate or BT: basal diet + calcium butyrate + tannin extract. The butyrate and tannin inclusion levels were 0.15% in the pre-starter phase and 0.075% in the starter phase.Incidence of diarrhea was monitored daily, and on d 14 and 35 of experiment, 1 animal from each experimental unit was slaughtered to collect intestinal samples.Results: No significant differences were observed for growth performance. The butyrate-and tannin-based additive resulted in reduced (p < 0.05) incidence of diarrhea in piglets during d 1 to 14 and d 1 to 35 in comparison with the other treatments. Piglets that consumed the diet containing the calcium-butyrate and tannin showed a lower (p < 0.05) crypt depth in the duodenum than those receiving the NC treatment at 14 d of experimentation. The BT treatment provided a lower (p < 0.05) immune-expression of COX-2 at 14 d and TNF-α at 35 d in the duodenum. Conclusion:Association between calcium butyrate and tannin extract resulted in a significant decrease in the incidence of diarrhea and inflammatory process in the duodenum of piglets.

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Section: Immunohistochemistrymentioning

confidence: 99%