Hiperparasitismo de Fusarium spp. em Austropuccinia psidii em Jambo-do-Pará

Lira, Vanessa Lopes; Santos, Ana Carla da Silva; Tiago, Patrícia Vieira; Oliveira, Neiva Tinti de; Moura, Romero Marinho de

doi:10.1590/0100-5405/187593

Cited by 3 publications

(1 citation statement)

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“…As a matter of consequence, the identification based on the sequencing of ITS region and others regions, such as EF-1α, has provided an alternative approach for higher detection and identification of Fusarium spp. [41][42][43].…”

Section: Discussionmentioning

confidence: 99%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

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The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1α) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil.

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Section: Discussionmentioning

confidence: 99%