COVID-19 diagnosis by RT-qPCR in alternative specimens

Gonçalves, Cássia Cristina Alves; Barroso, Shana Priscila Coutinho; Herlinger, Alice Laschuk; Galliez, Rafael de Mello; Almeida, Tailah Bernardo de; Boullosa, Lídia Theodoro; Nascimento, Érica Ramos dos Santos; Almeida, Jéssica Maciel de; Costa, Raissa Mirella dos Santos Cunha da; Paixão, Tatiana Monteiro da; Couceiro, José Nelson dos Santos Silva; Frauches, Thiago Silva; Souza, Wilson Rodrigues de; Costa, Andréa Ribeiro; Faffe, Débora S.; Leitão, Isabela de Carvalho; Silva, Bianca Ortiz da; Lira, Guilherme S; Almeida, Isabela Labarba Carvalho de; Ferreira, Orlando da Costa; Castiñeiras, Terezinha Marta Pereira Pinto; Mariani, Diana; Tanuri, Amílcar

doi:10.1590/0074-02760210085

Cited by 6 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data is in contrast with those reported by Marais 2021 [17], but support previous observations using the Panbio TM COVID-19 Ag RDT for other VOCs detection [29]. On the other hand, Lin 2022 reported no difference between nasal and saliva specimens for Omicron diagnosis [30].…”

Section: Discussionsupporting

confidence: 79%

Evaluation of the Panbio™ COVID-19 Antigen Rapid Diagnostic Test in subjects infected with Omicron using different specimens

Galiez

Bomfim

Mariani

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Community testing is a crucial tool for the early identification of SARS-CoV-2 infection and transmission control. The emergence of the highly mutated Omicron variant (B.1.1.259) raised concerns about its primary site of replication, impacting sample collection, and its detectability by rapid antigens tests. We tested the Antigen Rapid Diagnostic Test (Ag-RDT) performance using nasal, oral, and saliva specimens for COVID-19 diagnosis in 192 symptomatic individuals, using RT-qPCR from nasopharyngeal samples as control. Variant of Concern (VOC) investigation was determined by the 4Plex SARS-CoV-2 screening kit. SARS-CoV-2 positivity rate was 66.2%, with 99% of the positive samples showing an amplification profile consistent with that of the Omicron variant. Nasal Ag-RDT showed higher sensitivity (89%) than oral (12.6%) and saliva (22.1%) Ag-RDTs. Our data showed the good performance of the Ag-RDT in a pandemic scenario dominated by the Omicron VOC. Furthermore, our data also demonstrated that nasal specimens perform better than oral and saliva ones for Omicron Ag-RDT detection.

show abstract

Section: Discussionsupporting

confidence: 79%

Evaluation of the Panbio™ COVID-19 Antigen Rapid Diagnostic Test in subjects infected with Omicron using different specimens

Galiez

Bomfim

Mariani

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

COVID-19: the possibility, ways, mechanisms, and interruptions of mother-to-child transmission

Wang

Dong

2022

Arch Gynecol Obstet

View full text Add to dashboard Cite

Background In December 2019, novel coronavirus pneumonia was detected in Wuhan, Hubei Province, China, and as the epidemic spread, such cases emerged worldwide. Recently, the World Health Organization (WHO) named a new mutant Omicron (B.1.1.529), which disrupts the binding of most antibodies to the S protein and has a greater ability to break through the vaccine, posing a serious risk to population safety. Positive pregnant women give birth to positive newborns despite appropriate isolation measures taken by medical staff, suggesting that we may have vertical transmission of the novel coronavirus. This article analyzes and studies the possible vertical transmission path of the new coronavirus in the perinatal period of pregnant women and the antibody-dependent enhancement (ADE), and puts forward effective preventive measures for positive pregnant women to provide further reference for clinical work. Methods We searched multiple databases, including PubMed, CNKI, Google Scholar, WHO COVID-19 database, and CDC database. Search terms included COVID-19, SARS-CoV-2, vertical transmission, Omicron, Vaginal, Breast Feeding, Vaccine, Neonatal, Severe acute respiratory syndrome coronavirus, Pregnancy, and Semen. Selection criteria The following criteria were also met: (1) positive maternal novel coronavirus nucleic acid test; (2) reporting of neonatal outcome; (3) language in Chinese or English; (4) study date or location indicated; (5) no suspected or confirmed duplicated reports. Results There is evidence of vertical transmission, and the risk of possible vertical transmission is 5.7% (75/1314). The article listed four possible vertical transmission routes, namely placental transmission, vaginal upstream transmission, breastfeeding transmission and monocyte, and macrophage transmission route, with placental transmission being the most probable. Meanwhile, SARS-CoV-2 may also enter the placenta to infect the fetus through antibody-dependent enhanced substitution mechanism. We recommend three methods for early surveillance of vertical transmission, namely nucleic acid testing, antibody screening, and antigen testing, and analyze their advantages and disadvantages. Finally, the article provides recommendations in four areas: labor management, neonatal management, nosocomial infection prevention and control, and vaccination. As well as suggesting effective preventive measures for positive pregnant women and analyzing the advantages and disadvantages of vaccination, it is recommended that pregnant women should be vaccinated promptly, but considering that the vaccine may cause fever, it is recommended to consider vaccination cautiously in the first trimester of pregnancy. Conclusion The article concludes that vertical transmission is possible, with placental transmission being the most likely, and that the risk of possible vertical transmission is 5.7% (75/1314). Good personal protection, patien...

show abstract

The Effects of a Male Audience on Male and Female Mating Behaviour in the Blood-Sucking Bug Rhodnius prolixus

2022

View full text Add to dashboard Cite

COVID-19 diagnosis by RT-qPCR in alternative specimens

Cited by 6 publications

References 15 publications

Evaluation of the Panbio™ COVID-19 Antigen Rapid Diagnostic Test in subjects infected with Omicron using different specimens

Evaluation of the Panbio™ COVID-19 Antigen Rapid Diagnostic Test in subjects infected with Omicron using different specimens

COVID-19: the possibility, ways, mechanisms, and interruptions of mother-to-child transmission

The Effects of a Male Audience on Male and Female Mating Behaviour in the Blood-Sucking Bug Rhodnius prolixus

Contact Info

Product

Resources

About