Comparative genomics of pathogenic Leptospira interrogans serovar Canicola isolated from swine and human in Brazil

Moreno, Luisa Zanolli; Miraglia, Fabiana; Kremer, Frederico Schmitt; Eslabão, Marcus Redü; Dellagostin, Odir A.; Lilenbaum, Walter; Freitas, Júlio César de; Vasconcellos, Sílvio Arruda; Heinemann, Marcos Bryan; Moreno, Andréa Micke

doi:10.1590/0074-02760170119

Cited by 6 publications

(4 citation statements)

References 21 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although rodents have been considered the main urban hosts for leptospiral harboring and maintenance, particularly in slums ( 16 , 47 ), dogs and other animal species may be implicated in the local epidemiology of human disease ( 48 ). Leptospiral genotyping in human and rat infections in Seychelles, which has one of the highest worldwide incidence rates, has proposed other animal reservoirs ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

Spatial and Simultaneous Seroprevalence of Anti-Leptospira Antibodies in Owners and Their Domiciled Dogs in a Major City of Southern Brazil

et al. 2021

View full text Add to dashboard Cite

Although leptospirosis has been considered a major concern in urban areas, no study to date has spatially and simultaneously compared both owner and dog serology in households of major cities. Accordingly, the aim of the present study was to assess the seroprevalence of Leptospira antibodies, evaluate associated risk factors and conduct spatial analyses in 565 randomly selected households, which included 597 dog owners and 729 dogs in Londrina, Southern Brazil. Seropositivity by MAT were detected in in 11/597 (1.84%) owners and in 155/729 (21.26%) dogs. The risk factors were evaluated with logistic regression analysis and spatial factors and case distribution were evaluated with kernel density analyses. The sera of 14/155 (9.03%) dogs reacted for more than one serovar with the same titer. Canicola was the most frequent serogroup, detected in 3/11 (27.27%) owners and 76/155 (49.03%) dogs. The highest titer among the owners was 1:3,200 and was detected in the same household with a titer of 1:800 in the dog. Simultaneous owner-dog seropositivity was found in 7/565 (1.23%) households, with three reacted against serogroup Canicola. Positive owners were detected in 4/565 (0.70%) households and positive dogs were detected in 141/565 (24.95%) households. The associated risks of infection for dogs were different from those associated with infection in owners. Risk analyses for Canicola also identified specific factors of infection. Regardless of owner and dog cases were not statistically clustered, the kernel map has shown dog positivity occurrence in the same hot locations and near positive owners. The dependent variable analysis and logit model suggested a greater likelihood of peri-domiciliary contact with Leptospira. In conclusion, exposure to Leptospira infection was significantly higher in dogs than in their owners and human cases spatially overlapped dog cases, implicating dogs as potential environmental sentinels for this disease. In addition, the associated risk may vary according to serogroup, and the observed simultaneous Canicola seropositivity of owner and dog has suggested intradomicile-transmitted infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Spatial and Simultaneous Seroprevalence of Anti-Leptospira Antibodies in Owners and Their Domiciled Dogs in a Major City of Southern Brazil

et al. 2021

View full text Add to dashboard Cite

show abstract

“…As regard serovar Canicola, 0.45% (6/1332) of sera scored positive at titer 1:100/1:200 (3 at titer of 1:100 and 3 at titer of 1:200). Some studies demonstrated that swine could be infected by serovar Canicola and that intraspecies transmission is possible [42,45]. For this reason, pigs are considered a new possible potential maintenance host for serovar Canicola, although its real epidemiological role is not still clarified [46,47].…”

Section: Swinementioning

confidence: 99%

Epidemiology of leptospirosis in North-Central Italy: Fifteen years of serological data (2002–2016)

Bertelloni

Cilia

Turchi

et al. 2019

Comparative Immunology, Microbiology and Infectious Diseases

View full text Add to dashboard Cite

“…The two plasmids in the L. interrogans serogroup Canicola strains characterized in this study contained many novel genes, although the functions of most of these genes are unknown. High similarities were found between the sequences of plasmids from published genomes of three L. interrogans serogroup Canicola strains and the two strains in this study, whereas the two strains and L. borgpetersenii serovar Ballum showed low similarities (<20%), 34,49 suggesting that these plasmids play important roles in host adaptation of strains in different serogroups. Plasmids with extrachromosomal self-replicating activities and those that code their own cell-to-cell conjugal transfer systems are particularly important contributors to horizontal gene transfer among bacteria.…”

Section: Discussionmentioning

confidence: 53%

Comparative genomic analysis of Chinese human leptospirosis vaccine strain and circulating isolate

Zhang

Zhou

et al. 2020

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

Leptospira interrogans serogroup Canicola is one of the most important pathogens causing leptospirosis and is used as a vaccine strain of the current Chinese human leptospirosis vaccine. To characterize leptospiral pathogens, L. interrogans serogroup Canicola vaccine strain 611 and circulating isolate LJ178 from different hosts at different periods were sequenced using a combined strategy of Illumina X10 and PacBio technologies, and a comprehensive comparative analysis with other published Leptospira strains was conducted in this study. High levels of genomic similarities were observed between vaccine strain 611 and circulating isolate LJ178; both had two circular chromosomes and two circular extrachromosomal replicons. Compared with the strain 611 genome, 132 single nucleotide polymorphisms and 92 indels were found in strain LJ178. The larger lipopolysaccharide biosynthesis locus of serogroup Canicola was identified in both genomes. The phylogenetic analysis based on whole-genome sequences revealed that serogroup Canicola was not restricted to a specific host or geographic location, suggesting adaptive evolution associated with the ecologic diversity. In summary, our findings provide insights into a better molecular understanding of the component strains of human leptospirosis vaccine in China. Furthermore, these data detail the genetic composition and evolutionary relatedness of Leptospira strains that pose a health risk to humans.

show abstract

Comparative genomics of pathogenic Leptospira interrogans serovar Canicola isolated from swine and human in Brazil

Cited by 6 publications

References 21 publications

Spatial and Simultaneous Seroprevalence of Anti-Leptospira Antibodies in Owners and Their Domiciled Dogs in a Major City of Southern Brazil

Spatial and Simultaneous Seroprevalence of Anti-Leptospira Antibodies in Owners and Their Domiciled Dogs in a Major City of Southern Brazil

Epidemiology of leptospirosis in North-Central Italy: Fifteen years of serological data (2002–2016)

Comparative genomic analysis of Chinese human leptospirosis vaccine strain and circulating isolate

Contact Info

Product

Resources

About