Analysis of E2 gene integrity in HPV16 and HPV58 viruses isolated from women with cervical pathology

Abstract

Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16- and 31 HPV58-positive samples were analysed. E2 integrity was assumed when all fragments covering the E2 gene were amplified with specific polymerase chain reaction primers. Overall, in 59% of the samples, at least one fragment was not amplified in HPV16- (57%) and HPV58-positive samples (61%). Samples from high-grade squamous intraepithelial lesions had the highest frequency of E2 gene disruptions (73%), followed by samples from low-grade squamous intraepithelial lesions (63%) and, finally, samples from invasive cervical cancer (35%). Association between the integrity status of the E2 gene, and lesion grade was assessed by the chi-squared test applied to the combined set of viruses (p = 0.6555) or to populations of the same virus type (HPV58, p = 0.3101; HPV16, p = 0.3024). In conclusion, in this study, no association was found between the presence of E2 gene disruptions and the grade of cervical lesions caused by HPV16 and HPV58.

Determinants of cervical cancer screening service utilization among women attending healthcare services in Amhara region referral hospitals: a case–control study

“…Cervical cancer is the fourth most common cancer accounting for 6.6% among women worldwide [17]. About 85% of the global burden and 87% of mortalities occur in developing countries.…”

“…In Ethiopia, only 0.6% of all women, 1.6% of urban, and 0.4% of rural women aged 18-69 years screened every three years [25,26]. The major factors associated with low cervical cancer screening were inadequate knowledge about the disease process, available screening methods, and client's negative attitudes toward the procedure [17,27,28]. In Ethiopia, screening is usually conducted only when a woman seeks medical care for other reasons [29].…”

Factors Protecting Male and Female Adolescents in Mexico From Human Papillomavirus Infection

“…HPV infection, particularly the oncogenic type, poses significant health risk during adolescence due to its high transmission rate during the first sexual encounter (Castellsagué et al, 2014). Exposure to HPV can occur via anal, oral, or vaginal sexual relations (Gonzalez-Losa et al, 2016). An example of high-risk exposure to HPV occurs when adolescents have first sexual relationships with sexually experienced partner, even though currently in monogamous sexual relationships (Winer et al, 2008; Zou et al, 2014).…”