Comparative growth of spotted fever group Rickettsia spp. strains in Vero cells

Silva, Arannadia Barbosa; Duarte, Myrian Morato; Vizzoni, Vinicius Figueiredo; Duré, Ana Íris de Lima; Montenegro, Diego; Nogueira, Rita de Maria Seabra; Soares, Carlos A. G.; Machado-Ferreira, Erik

doi:10.1590/0074-02760160093

Cited by 1 publication

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References 22 publications

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“…Espécie do GFM, amplamente distribuída no mundo, presente em todos os continentes, exceto Oceania e Antártida, mas de potencial patogênico desconhecido, sem casos de infecção humana registrados (PAROLA et al, 2013;SILVA et al, 2016). Descoberta e isolada nos Estados Unidos (BURGDORFER et al, 1975) ), e a mais frequente em carrapatos no Brasil (LABRUNA et al, 2007b), havendo evidência de infecção em capivaras (PACHECO et al, 2007), cães e cavalos (FORTES et al, 2010;AMORIM et al, 2013) no país.…”

Section: Rickettsia Rhipicephaliunclassified

Ocorrência de infecção por Rickettsia rickettsii em hospedeiros do carrapato-estrela no Campus \"Luiz de Queiroz\"

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Brazilian Spotted Fever (BSF) is an infectious disease caused by Rickettsia rickettsii, transmitted by the tick Amblyomma sculptum outside the metropolitan region in the State of São Paulo, where horses and capybaras are used as sentinels for BSF as they are considered primary hosts for this tick. This survey aimed to verify the occurrence of antibodies against Rickettsia rickettsii in sentinels (horses) and potential sentinels (sheep, cattle and opossums) for BSF that lives in different environments of the "Luiz de Queiroz" Campus, University of São Paulo, in Piracicaba, SP, Brazil. A total of 156 host were sampled between February of 2017 and January of 2018; 48 that live in urban areas, with no presence or transit of capybaras (15 horses and 33 opossums), 60 sheep that regularly graze on capybarafree pastures adjacent to a riparian forest occupied by these rodents, and 48 that occupy, cross or regularly graze on pastures (31 cows and six horses) or riparian forests (11 opossums) that are areas used by capybaras. Whenever possible, host sampling included ectoparasites collection. Sera obtained after centrifugation of the collected blood was analyzed in two steps, screening and titration, by the indirect immunofluorescence assay technique (IFA). All samples were tested against R. rickettsii during the screening, and those that presented antibodies titers ≥ 64 were considered positive. Positive samples were diluted during titration and tested against R. rickettsii, R. parkeri, R. bellii, R. ambyommatis and R. rhipicephali for determination of maximum antibodies titers for each species. Of the 156 tested samples, 15 were seropositive, 1/60 sheep, 1/31 cows, 7/21 horses and 6/44 opossums; antibodies endpoint titers ranged from 64 to 4.096. Possible antigen involved in a homologous reaction (PAIHR) was determined for six samples, one horse (R. bellii), four opossums and one sheep (R. rickettsii). The ticks Amblyomma ovale, Amblyomma dubitatum, Amblyomma sculptum, Dermacentor nitens and Rhipicephalus microplus, and the fleas Ctenocephalides felis and Siphonaptera: Rhopalopsyllidae cf. Rhopalopsyllus were collected from the sampled hosts. 8 Results showed that horses and opossums may be used as sentinels for BSF in the "Luiz de Queiroz" Campus, but the sheep do not. It wasn't possible to determine if the cows may be used as sentinels. R. bellii, or a very closely related microorganism, occurs in the urban area of the "Luiz de Queiroz" Campus and is capable to infect horses. R. rickettsii, or a very closely related microorganism, occurs in capybaratransit areas of the "Luiz de Queiroz" Campus.

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Section: Rickettsia Rhipicephaliunclassified

Ocorrência de infecção por Rickettsia rickettsii em hospedeiros do carrapato-estrela no Campus \"Luiz de Queiroz\"

Ortiz¹

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Comparative growth of spotted fever group Rickettsia spp. strains in Vero cells

Cited by 1 publication

References 22 publications

Ocorrência de infecção por Rickettsia rickettsii em hospedeiros do carrapato-estrela no Campus \"Luiz de Queiroz\"

Ocorrência de infecção por Rickettsia rickettsii em hospedeiros do carrapato-estrela no Campus \"Luiz de Queiroz\"

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