Molecular characterization of Candida spp. isolates from patients with bloodstream infections

Xavier, Paula Cristhina Niz; Chang, Marilene Rodrigues; Paula, Claudete Rodrigues; Matsumoto, Flavia E.; Asensi, Marise Dutra; Matos, Maria de Fátima Cepa; Andreotti, Renato

doi:10.1590/0037-8682-1718-2013

Rev. Soc. Bras. Med. Trop.

2013

DOI: 10.1590/0037-8682-1718-2013

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Molecular characterization of Candida spp. isolates from patients with bloodstream infections

Paula Cristhina Niz Xavier

Marilene Rodrigues Chang

Claudete Rodrigues Paula

et al.

Abstract: Revista da Sociedade Brasileira de Medicina Tropical 46(6):786-787, Nov-Dec, 2013http://dx

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Cited by 2 publications

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“…Compared with the scarce data from previous studies conducted in the Midwest region of Brazil 12,14,15 , our results show an increase in the percentage of isolates resistant to antifungal azoles.…”

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confidence: 89%

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Variability in the clinical distributions of Candida species and the emergence of azole-resistant non-Candida albicans species in public hospitals in the Midwest region of Brazil

Mattos

Rodrigues

Oliveira

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Incidence and antifungal susceptibility of Candida spp. from two teaching public hospitals are described. Methods: The minimum inhibitory concentrations of fl uconazole, voriconazole, itraconazole, and amphotericin B were determined using Clinical Laboratory Standard Institute broth microdilution and genomic differentiation using PCR. Results: Of 221 Candida isolates, 50.2% were obtained from intensive care unit patients; 71.5% were recovered from urine and 9.1% from bloodstream samples. Candida parapsilosis sensu stricto was the most common candidemia agent. Conclusions: We observed variations in Candida species distribution in hospitals in the same geographic region and documented the emergence of non-C. albicans species resistant to azoles.

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confidence: 89%

“…Previous studies 2,12,14,15 indicate that Candida isolates of various species were susceptible to amphotericin B. In contrast, 132 (57.6%) Candida spp.…”

mentioning

confidence: 98%

Variability in the clinical distributions of Candida species and the emergence of azole-resistant non-Candida albicans species in public hospitals in the Midwest region of Brazil

Mattos

Rodrigues

Oliveira

et al. 2017

Rev. Soc. Bras. Med. Trop.

Self Cite

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Multicenter Evaluation of Candida QuickFISH BC for Identification of Candida Species Directly from Blood Culture Bottles

et al. 2015

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A total of 415 samples contained a single fungal species, with C. glabrata (n ‫؍‬ 129; 30.8%) being the most common isolate, followed by C. albicans (n ‫؍‬ 125; 29.8%), C. parapsilosis (n ‫؍‬ 59; 14.1%), C. tropicalis (n ‫؍‬ 37; 8.8%), and C. krusei (n ‫؍‬ 17; 4.1%). The overall agreement (with range for the three major Candida species) between the two methods was 99.3% (98.3 to 100%), with a sensitivity of 99.7% (98.3 to 100%) and a specificity of 98.0% (99.4 to 100%). This study showed that Candida QuickFISH BC is a rapid and accurate method for identifying C. albicans, C. glabrata, and C. parapsilosis, the three most common Candida species causing BSI, directly from blood culture bottles. Candida species are ranked as the third or fourth most common etiologic agents associated with nosocomial bloodstream infections (1, 2). Mortality rates are estimated to be as high as 45% (3, 4), due in part to slow diagnostic methods and to inappropriate initial antifungal treatment (5, 6). Candida albicans and other yeasts reside as commensals of the skin and mucous membranes of the gastrointestinal and genitourinary tracts, causing infection when the host becomes debilitated or immunocompromised. Risk factors for invasive candidiasis include central venous catheters, parenteral nutrition, organ transplantation, hemodialysis, surgery, burns, long-term stay in an intensive care unit, and previous administration of broad spectrum antimicrobial and immunosuppressive agents (7,8).The most common Candida species found in healthy individuals include C. albicans, C. glabrata, C. tropicalis, and C. parapsilosis (9). These species cause more than 90% of invasive infections (10). However, the prevalence of these different Candida species varies widely geographically, and their antifungal susceptibilities are species specific (11). Consequently, rapid, accurate identification of the causative Candida species is critical for successful treatment.Currently, blood culture is the gold standard method for the diagnosis of fungal bloodstream infection (12). When growth is detected, blood culture medium is drawn for Gram stain and subculture on agar plates for subsequent phenotypic identification (13), which typically takes at least 72 h (14). To overcome this relatively long turnaround time of identification, several molecular methods have been evaluated for identification of yeasts directly from blood culture bottles such as real-time PCR, matrixassisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, and peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) (15,16). A method to detect Candida directly from patient's blood specimens within 3 h has also been developed (17, 18).The first generation of yeast PNA-FISH (AdvanDx, Woburn, MA) distinguishes between the five commonest Candida species directly from positive blood cultures within 90 min. The method uses fluorescence-labeled probes to complement species-specific rRNA sequences: C. albicans and C. parapsilosis fluoresce green, C. tropicalis fluor...

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Molecular characterization of Candida spp. isolates from patients with bloodstream infections

Abstract: Revista da Sociedade Brasileira de Medicina Tropical 46(6):786-787, Nov-Dec, 2013http://dx

Cited by 2 publications

References 3 publications

Variability in the clinical distributions of Candida species and the emergence of azole-resistant non-Candida albicans species in public hospitals in the Midwest region of Brazil

Variability in the clinical distributions of Candida species and the emergence of azole-resistant non-Candida albicans species in public hospitals in the Midwest region of Brazil

Multicenter Evaluation of Candida QuickFISH BC for Identification of Candida Species Directly from Blood Culture Bottles

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