Profile of the Trypanosoma cruzi vector infestation in Jaboticatubas, State of Minas Gerais, Brazil

Belisário, Carlota Josefovicz; Dias, João Víctor Leite; Diotaiuti, Liléia

doi:10.1590/0037-8682-1712-2013

Cited by 10 publications

(5 citation statements)

References 4 publications

(4 reference statements)

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“…A study performed by Paula et al [ 48 ] in Minas Gerais state demonstrated that P. megistus exhibited a T. cruzi infection rate of 8.3% and that from among a total of 147 specimens identified in Brasilia Federal District, only three (1.45%) presented flagellate forms [ 36 ]. These results reinforce the low T. cruzi infection rate exhibited by P. megistus , which can be justified by its correlation with chicken houses in the peridomicile [ 5 , 48 , 69 ]. However, although P. megistus has been shown to represents an effective CD vector and to be present in domiciles in Brazilian Cerrado and Caatinga biomes, in the Atlantic rainforest it is predominantly sylvatic, as was also observed by Barbosa et al [ 3 ] in Santa Catarina state.…”

Section: Discussionsupporting

confidence: 72%

Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil

et al. 2018

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Background: In rural areas of Espírito Santo state, southeast Brazil, triatomine species attracted by light frequently invade residences. The aim of this study was to investigate the Trypanosoma cruzi discrete typing units (DTUs) harbored by these triatomines. Methods: Triatomine’s intestinal contents were examined, inoculated in mice, and the positive samples were cultivated. Flagellates obtained from infected mice hemoculture were submitted to DNA extraction using a salting-out method and to TcSC5D gene amplification. The amplified samples were sequenced, and polymorphism was analyzed for DTU identification. Results: Three hundred and ninety-four triatomines were identified: Triatoma vitticeps (90.03%), Panstrongylus geniculatus (8.89%), Panstrongylus megistus (0.54%), Panstrongylus diasi (0.27%), and Triatoma tibiamaculata (0.27%). Among the specimens, 251/394 (67.65%) presented flagellated forms similar to T. cruzi. After triatomine intestinal content inoculation into mice, 134 mice presented T. cruzi-like trypomastigotes from Tr. vitticeps and P. geniculatus and 89 samples were positive in hemoculture. Sixty-two samples were analyzed for the TcSC5D gene and TcI, TcII, TcIII, and TcIV DTUs were identified. Conclusions: We observed T. cruzi DTU diversity in Tr. vitticeps and P. geniculatus, which showed the predominance of TcII and occurrence of TcI, TcIII and TcIV. Triatomines presented high T. cruzi infection rates. Since little is known regarding the possible mammalian hosts that maintain the T. cruzi cycle, further studies are necessary to obtain a better understanding of the parasite transmission cycle in this region.

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Section: Discussionsupporting

confidence: 72%

Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil

et al. 2018

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“…P. megistus is widely distributed in Brazil, from the northeast to the south 8 . In Minas Gerais, this species also has a wide dispersal area and is considered the most important triatomine in this state 17 . In the health macroregion of Governador Valadares, P. megistus was the second most common species, accounting for 18.9% of specimens, with a T. cruzi infection rate of 37.8%.…”

Section: Discussionmentioning

confidence: 99%

Entomological surveillance of Chagas disease in the East of Minas Gerais region, Brazil.

Rezende

Lana

Diotaiuti³

et al. 2022

Rev. Soc. Bras. Med. Trop.

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Background: After decentralizing the actions of the Chagas Disease Control Program (CDCP) in Brazil, municipalities were now responsible for control measures against this endemic, supervised by the Regional Health Superintendencies (RHS). We aimed to evaluate the recent entomological surveillance of Chagas disease in the Regional Health Superintendence of Governador Valadares (RHS/GV) from 2014 to 2019. Methods:Triatomines captured by residents during entomological surveillance were sent to the reference laboratory, where the species and evolutionary stages were identified, place of capture, and presence of Trypanosoma cruzi. A database was created, and the following were calculated: the rate of infection by T. cruzi (overall rate and rate by species), monthly seasonality, spatial distribution of species, number of captures, and infected triatomines/health microregions. Results:We identified 1,708 insects; 1,506 (88.2%) were triatomines, most were adult instars (n=1,469), and few were nymphs (n=37). The identified species were Triatoma vitticeps, Panstrongylus megistus, Panstrongylus diasi, Rhodnius neglectus, and Panstrongylus geniculatus. The first three were most frequently captured and distributed throughout the study area. Most bugs were captured intradomicile (72.5%), mainly in the second semester, between September and November, with an average infection rate of 41.5% (predominantly T. vitticeps, 49.2%). All municipalities sent triatomines, especially in the microregions of Governador Valadares.Conclusions: These data reinforce the need and importance of improving Chagas disease control measures in the region to establish active and participatory entomological surveillance.

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“…Fifteen specimens of P. megistus captured in the municipality of Jaboticatubas, Minas Gerais, Brazil (19°30′50″S, 43°44′42″W) by Belisário et al [ 30 ] were used. These samples were divided into two groups in order to evaluate intra-populational variability: nine insects from the locality Fazenda Santo Antônio (group I); and one insect each from the following localities: Barreiro do Papagaio, Fazenda Espada, Capão Grande II, Fazenda Borges, Guarazinho, and Fazenda Boiça (group II).…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of microsatellite markers for population genetic studies of Panstrongylus megistus (Burmeister, 1835) (Triatominae: Reduviidae)

et al. 2021

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Background Panstrongylus megistus is the most important vector of Chagas disease in Brazil. Studies show that the principal factor hindering the control of triatomines is reinfestation of houses previously treated with insecticides. Studies at the microgeographic level are therefore necessary to better understand these events. However, an efficient molecular marker is not yet available for carrying out such analyses in this species. The aim of the present study was to identify and characterize microsatellite loci for future population genetic studies of P. megistus. Methods This study work consisted of five stages: (i) sequencing of genomic DNA; (ii) assembly and selection of contigs containing microsatellites; (iii) validation of amplification and evaluation of polymorphic loci; (iv) standardization of the polymorphic loci; and (v) verification of cross-amplification with other triatomine species. Results Sequencing of males and females generated 7,908,463 contigs with a total length of 2,043,422,613 bp. A total of 2,043,690 regions with microsatellites in 1,441,091 contigs were obtained, with mononucleotide repeats being the most abundant class. From a panel of 96 loci it was possible to visualize polymorphisms in 64.55% of the loci. Of the 20 loci genotyped, the number of alleles varied from two to nine with an average of 4.9. Cross-amplification with other species of triatomines was observed in 13 of the loci. Conclusions Due to the high number of alleles encountered, polymorphism and the capacity to amplify from geographically distant populations, the microsatellites described here show promise for utilization in population genetic studies of P. megistus. Graphic abstract

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Profile of the Trypanosoma cruzi vector infestation in Jaboticatubas, State of Minas Gerais, Brazil

Cited by 10 publications

References 4 publications

Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil

Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil

Entomological surveillance of Chagas disease in the East of Minas Gerais region, Brazil.

Identification and characterization of microsatellite markers for population genetic studies of Panstrongylus megistus (Burmeister, 1835) (Triatominae: Reduviidae)

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