Leishmania V. braziliensis infection in asymptomatic domestic animals within an endemic region in the Northeast of Brazil

Abstract

Background: American cutaneous leishmaniasis is a commonly neglected, vector-borne tropical parasitic disease that is a major public health concern in Brazil. Leishmania (Viannia) braziliensis is the main species associated with the disease. Accurate diagnosis is based on epidemiological surveillance, clinical assessment, and laboratory testing. Leishmania (V.) braziliensis has been detected in several wild and synanthropic mammals. Their epidemiological role has not been entirely elucidated. This study aimed to assess potential L. braziliensis infections in asymptomatic domestic animals, by molecular and serological testing in endemic areas, in the metropolitan region of Recife.Methods: Blood samples and conjunctival fluids were collected from 232 animals (canids, felids, equines, and caprines) for the detection of L. braziliensis using molecular tests (conventional and real-time polymerase chain reaction [PCR and qPCR]). For immunological detection, blood samples from 115 dogs were assessed using enzyme-linked immunosorbent assay.Results: Real-time quantitative PCR showed positive results for blood and conjunctival samples in all investigated species. The results of the blood and conjunctival samples were 68.2% and 26.9% in Canis familiaris, 100% and 41.7% in Felis catus, 77.3% and 30.8% in Equus caballus/Equus asinus, and 50% and 33.3% in Capra hircus samples, respectively. Conclusions:Results from this study adds valuable information to our understanding of the role of asymptomatic domestic animals, L. braziliensis life cycle, and American cutaneous leishmaniasis in Northeast Brazil.

Chemokine receptors on human regulatory T cells during cutaneous leishmaniasis

“…Leishmania (Viannia) braziliensis is the main endemic species causing CL in North and Northeast of Brazil. 3 Leishmania injection in the skin by sand flies triggers complex cellular and molecular interactions that may result in sub-clinical (SC) infection or in disease manifestation with single or Maria Carolina Accioly Brelaz de Castro and Rafael de Freitas e Silva have contributed equally to this work. multiple skin lesions.…”

“…CL affects mainly poor people from low‐income countries and is associated with poor housing, population displacement, malnutrition and compromised immune system. Leishmania (Viannia) braziliensis is the main endemic species causing CL in North and Northeast of Brazil 3 . Leishmania injection in the skin by sand flies triggers complex cellular and molecular interactions that may result in sub‐clinical (SC) infection or in disease manifestation with single or multiple skin lesions 4,5 .…”