Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Lima, Giselle Jucá de; Scavuzzi, Alexsandra Maria Lima; Beltrão, Elizabeth Maria Bispo; Firmo, Elza Ferreira; Oliveira, Érica Maria de; Oliveira, Sibele Ribeiro de; Rezende, Antônio Mauro; Lópes, Ana Catarina de Souza

doi:10.1590/0037-8682-0526-2019

Cited by 11 publications

(6 citation statements)

References 16 publications

(18 reference statements)

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“…The non-Tn4401 variant of the present study resembles the NTE KPC− IId variant (Figure 2). These ndings corroborate the results obtained by our research group that demonstrated the presence of the bla KPC−2 gene in the non-Tn4401 element (NTE KPC− IId) [18,30], which is transported by small, mobilized and promiscuous plasmids of the IncQ1 type. These data indicate that this type of plasmid may have been responsible for the spread of the bla KPC−2 gene among K. pneumoniae in hospital patients in Recife, Brazil.…”

Section: Discussionsupporting

confidence: 92%

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Alves

Scavuzzi

Beltrão

et al. 2021

Preprint

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To characterize phenotypically and genotypically an isolate of Multidrug Resistant (MDR) K. pneumoniae from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated by using PCR and sequencing the amplicons and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all β-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variant blaNDM-7 associated with blaKPC-2 and the cps, wabG, fimH, mrkD and entB virulence genes were identified. The analysis of plasmid revealed the presence of blaCTX-M15, aac(3)-IVa, aph(3')-Ia, aph(4)-Ia, aac(6')ib-cr, mph(A) and catB3, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the blaNDM-7 gene in Brazil and we suggest that this variant is located in IncX3. These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.

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Section: Discussionsupporting

confidence: 92%

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Alves

Scavuzzi

Beltrão

et al. 2021

Preprint

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“…Contrary to previous studies reporting IncF plasmid group in E.coli to carry resistance and virulence genes often [34] the present study, however shows IncQ1 carried the highest number of both resistance and virulence genes in E.coli, but the finding is in line with study conducted in Brazil [35,36]. This is possibly due to the fact that IncQ1 plasmids have high-level mobility, stability, replication at high copy number and transferred in wide range of bacterial species through conjugative plasmids [37][38][39][40].…”

Section: Discussionsupporting

confidence: 84%

“…But the finding is in line with study conducted in Brazil [35,36]. This is possibly due to the fact that IncQ1 plasmids have high-level mobility, stability, replication at high copy number and transferred in wide range of bacterial species through conjugative plasmids [37][38][39][40].…”

Section: Discussionsupporting

confidence: 84%

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro, Tanzania

Sengeruan

Zwetselaar²,

Kumburu³

et al. 2022

Preprint

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Plasmids are infectious double stranded DNA molecules that are found within bacteria. Horizontal gene transfer promotes successful spread of different types of plasmids within or among bacteria species, making their detection an important task for guiding clinical treatment. We used whole genome sequenced data to determine the prevalence of plasmid replicons in clinical bacterial isolates, the presence of resistance and virulence genes in plasmids, and the relationship between resistance and virulence genes within each plasmid. All bacterial sequences were de novo assembled using Unicycler before extraction of plasmids. Assembly graphs were submitted to Gplas+plasflow for plasmid prediction. The predicted plasmid components were validated using PlasmidFinder. A total of 159 (56.2%) out of 283 bacterial isolates were found to carry plasmids, with E. coli, K. pneumoniae and S. aureus being the most prevalent plasmid carriers. A total of 27 (87.1%) combined plasmids were found to carry both resistance and virulence genes compared to 4 (12.9%) single plasmids. No statistically significant correlation was found between the number of antimicrobial resistance and virulence genes in plasmids (r =-0.25, p > 0.05). Our findings show a relatively high proportion of plasmid-carrying isolates suggesting selection pressure due to antibiotic use in the hospital. Co-occurrence of antibiotic resistance and virulence genes in clinical isolates is a public health relevant problem needing attention.

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“…Contrary to previous studies reporting IncF plasmid replicon type in E. coli to often carry resistance and virulence genes [33] , the present study shows IncQ1 replicon type carried the highest number of both resistance and virulence genes in E. coli , and the finding is in line with study conducted in Brazil [ 34 , 35 ]. This is possibly due to the fact that IncQ1 replicon type has high-level mobility, stability, and replication at high copy number and is transferred in a wide range of bacterial species through conjugative plasmids [36][37][38][39] .…”

Section: Discussionmentioning

confidence: 99%

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro region, Tanzania

Sengeruan

Zwetselaar²,

Kumburu³

et al. 2022

Journal of Global Antimicrobial Resistance

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Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Cited by 11 publications

References 16 publications

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro, Tanzania

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro region, Tanzania

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