Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Abstract

To characterize phenotypically and genotypically an isolate of Multidrug Resistant (MDR) K. pneumoniae from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated by using PCR and sequencing the amplicons and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all β-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variant blaNDM-7 associated with blaKPC-2 and the cps, wabG, fimH, mrkD and entB virulence genes were identified. The analysis of plasmid revealed the presence of blaCTX-M15, aac(3)-IVa, aph(3')-Ia, aph(4)-Ia, aac(6')ib-cr, mph(A) and catB3, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the blaNDM-7 gene in Brazil and we suggest that this variant is located in IncX3. These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.