Report of new cases of Trypanosoma caninum in Brazil

Oliveira, Tatiana da Silva Fonseca de; Barros, Juliana H. S.; Perez, Taliha Dias; Figueiredo, Fabiano Borges; Júnior, Artur Augusto Velho Mendes; Madeira, Maria de Fátima

doi:10.1590/0037-8682-0307-2014

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…The use of specific T. caninum primers might be more effective for its detection, as described for LVC. In this study, there was no association of T. caninum or L. infantum infection with age, breed, and sex, which is in agreement with previous findings in cases of visceral leishmaniasis (Almeida et al, 2009;Oliveira et al, 2010), while T. caninum has been detected in asymptomatic dogs aged 3 to 5 years (Oliveira et al, 2015). Nevertheless, the present T. caninum infection rate should be accepted cautiously, due to the low number of infected dogs.…”

Section: Discussionsupporting

confidence: 91%

“…In this scenario, L. infantum infection has important public health implications (Madeira et al, 2006) due to the relevance of dogs as a reservoir of zoonotic visceral leishmaniasis. The recent isolation of T. caninum from dogs' skin samples in endemic areas for visceral leishmaniasis has called attention to this protozoan (Barros et al, 2012;Oliveira et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…T. caninum has been isolated only from the skin so far, a factor that is unusual of the Trypanosoma genus, and it shares the same reservoir with L. infantum, the infectious agent of visceral leishmaniasis (VL). It has been isolated from several regions of Brazil (Alves et al, 2012;Oliveira et al, 2015). These aspects raise some public health concerns, especially the risk of erroneous diagnoses of LVC in areas of sympatry of L. infantum and T. caninum (Almeida et al, 2011;Alves et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Makino

Moreira

Bezerra

et al. 2020

Rev. Bras. Parasitol. Vet.

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Leishmania infantum is a trypanosomatid that causes parasitic dermatopathy in dogs. Trypanosoma caninum is another trypanosomatid, which infects the skin of dogs, although cutaneous abnormalities are absent. This study aimed to investigate the occurrence of T. caninum infection and its associated cutaneous and histological changes and compare it with the occurrence of L. infantum infection in dogs. The study included 150 dogs, of which T. caninum infection was identified in 3 (2%) and L. infantum infection in 15 (10%) of them, with no association (p>0.05) of these infections with the breed, gender, age, or cutaneous abnormalities. The cutaneous abnormalities were based on 1 (4.8%) and 12 (57.1%) dogs infected by T. caninum and L. infantum, respectively. The dermatohistopathological abnormalities in the dogs infected with T. caninum included mild perivascular lymphohistioplasmacytic infiltrates in the clinically asymptomatic ones, while in those with dermatological abnormalities, acanthosis, epidermal orthokeratotic hyperkeratosis, melanomacrophages, and co-infection with Microsporum sp. and Trichophyton sp. were observed. InL. infantum infected, the histopathological findings included chronic granulomatous inflammatory infiltrates and structures compatible with amastigotes. Despite the low frequency of T. caninum infection, our findings suggest that this trypanosomatid, unlike L. infantum, does not cause any macroscopic skin abnormalities.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Makino

Moreira

Bezerra

et al. 2020

Rev. Bras. Parasitol. Vet.

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“…cruzi lysates or whole-cell epimastigote homogenates as antigenic matrices [10,13–15]. This complex antigenic mixture of the variable component is highly sensitive and some drawbacks have been already described, such as difficult standardization, low specificity, and cross-reactivity with Leishmania spp., and other trypanosomatids [16,17]. Therefore, accurate serological diagnostic tests are needed to fill this gap.…”

Section: Introductionmentioning

confidence: 99%

Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs

Leony

Freitas

Del-Rei³

et al. 2019

PLoS Negl Trop Dis

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Background Dogs are considered sentinels in areas of Trypanosoma cruzi transmission risk to humans. ELISA is generally the method of choice for diagnosing T . cruzi exposure in dogs, but its performance substantially depends on the antigenic matrix employed. In previous studies, our group has developed four chimeric antigens (IBMP-8.1, 8.2, 8.3, and 8.4) and evaluated their potential for diagnosing T . cruzi exposure in humans. For human sera, these chimeric antigens presented superior diagnostic performances as compared to commercial tests available in Brazil, Spain, and Argentina. Therefore, in this study we have evaluated the potential of these antigenic proteins for detection of anti- T . cruzi IgG antibodies in dog sera. Methodology/Principal findings The IBMP-ELISA assays were optimized by checkerboard titration. Subsequently, the diagnostic potential was validated through analysis of ROC curves and the performance of the tests was determined using double entry tables. Cross-reactivity was also evaluated for babesiosis, ehrlichiosis, dirofilariosis, anaplasmosis, and visceral leishmaniasis. Best performance was shown by IBMP-8.3 and IBMP-8.4, although all four antigens demonstrated a high diagnostic performance with 46 positive and 149 negative samples tested. IBMP-8.3 demonstrated 100% sensitivity, followed by IBMP-8.4 (96.7–100%), IBMP-8.2 (73.3–87.5%), and IBMP-8.1 (50–100%). The highest specificities were achieved with IBMP-8.2 (100%) and IBMP-8.4 (100%), followed by IBMP-8.3 (96.7–97.5%) and IBMP 8.1 (89.1–100%). Conclusions/Significance The use of chimeric antigenic matrices in immunoassays for anti- T . cruzi IgG antibody detection in sera of infected dogs was shown to be a promising tool for veterinary diagnosis and epidemiological studies. The chimeric antigens used in this work allowed also to overcome the common hurdles related to serodiagnosis of T . cruzi infection, especially regarding variation of efficiency parameters according to different strains and cross-reactivity with other infectious diseases.

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“…It is interesting to note that all the 16 samples with conflicting results between the two tests originated from the dogs residing in areas with only occasional occurrences of CVL. Therefore, the possibility of interference from other infectious agents in these areas, including Trypanosoma cruzi, Trypanosoma caninum, Toxoplasma gondii, Neospora caninum, Babesia canis, and Ehrlichia canis, as previously observed 11,17,18 , must be considered. This possibility is reinforced by the reported 47% canine seroprevalence of parasites transmitted by ticks in the eastern region of Rio de Janeiro 19 and 80% seroprevalence in the surrounding area of the natural reserve in the state of Mato Grosso (unpublished data).…”

Section: Discussionmentioning

confidence: 98%

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Souza

Silva

Labarthe

2019

Rev. Soc. Bras. Med. Trop.

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Introduction: Visceral leishmaniasis is a disease that affects humans, wildlife, and domestic species. Since dogs play a key role in urban Leishmania spp. transmission, the Brazilian government maintains the Monitoring and Control Program of Visceral Leishmaniasis (VLMCP) in endemic regions, which promotes awareness campaigns aiming to enhance the control of the infection. The VLMCP recommends the Dual Path Platform (DPP ® ) canine visceral leishmaniasis test (Bio-Manguinhos, Brazil) for screening and enzyme-linked immunosorbent assay to confirm the infection. The DPP ® test is produced and distributed by the Health Ministry to the Municipal Health Centers responsible for the local VLMCP. The test is not available to all the clinics, forcing some veterinarians to use other rapid tests for screening and diagnosis of this disease in their daily routine. Methods: The present study was conducted to compare the performance of the DPP ® and SNAP ® tests using sera from the dogs with confirmed infections of L. infantum and from the dogs with no previous testing, residing in areas with a low Leishmania infection. Results: There was 97.0% agreement between the two tests. Sensitivity and specificity of the SNAP ® test were 96.3% and 100%, respectively. Agreement between both the antibody tests and the parasitological detection methods was 96.8%. The DPP ® test had 95.8% sensitivity and 100% specificity. Conclusions: The SNAP ® and the DPP ® tests were virtually equivalent in terms of detection of canine antibodies against L. infantum, and both the tests demonstrated high and similar levels of sensitivity and specificity.

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Report of new cases of Trypanosoma caninum in Brazil

Cited by 6 publications

References 12 publications

Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

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