Integrative literature review of the reported uses of serological tests in leprosy management

Fabri, Angélica da Conceição Oliveira Coelho; Carvalho, Ana Paula Mendes; Vieira, Nayara Figueiredo; Bueno, Isabela de Caux; Rodrigues, Rayssa Nogueira; Monteiro, Thayenne Barrozo Mota; Correa-Oliveira, Rodrigo; Duthie, Malcolm S.; Lana, Francisco Carlos Félix

doi:10.1590/0037-8682-0226-2015

Cited by 15 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…O M. leprae contém em sua parede celular, importantes componentes antigênicos da resposta imune do hospedeiro, incluindo o glicolipídio fenólico I (PGL-I), que estimula uma potente resposta de anticorpos IgM relacionada à carga bacilar dos pacientes. São vários os estudos que discutem o uso da sorologia como uma ferramenta para auxiliar no diagnóstico de hanseníase 13,14,25 26 . Outro estudo detectou que os anticorpos IgM, IgG e IgA anti-PGL-I podem apresentar competição ou até inibição da reação antígeno-anticorpo 27 .…”

Section: Discussionunclassified

“…A dificuldade de se obter um meio de cultura que auxilie no cultivo e isolamento do M. leprae dificulta o diagnóstico laboratorial. Em linhas gerais, o diagnóstico de um caso de hanseníase é essencialmente clínico e epidemiológico, além da detecção microbiológica e/ou molecular da bactéria e pela avaliação sorológica [13][14][15] . Os testes disponíveis hoje no mercado têm valor diagnóstico limitado, pois são capazes de detectar cerca de 90 a 100% dos doentes MB e somente 40 a 60% dos PB.…”

unclassified

See 1 more Smart Citation

Avaliação do desempenho de um teste rápido imunocromatográfico no diagnóstico de hanseníase em uma região endêmica no norte do Brasil

et al. 2018

View full text Add to dashboard Cite

Section: Discussionunclassified

unclassified

Avaliação do desempenho de um teste rápido imunocromatográfico no diagnóstico de hanseníase em uma região endêmica no norte do Brasil

et al. 2018

View full text Add to dashboard Cite

“…Researchers have come up with several advanced molecular methods including detection of specific markers of ML. Antibodies directed against the ML-specific phenolic glycolipid I (PGL-I) and leprosy IDRI diagnostic-1 (LID-1) make the basis of many serological tests 7 . Hooij et al 8 evaluated two recently designed field-friendly lateral flow assays (LFAs- the OnSite Leprosy Ab Rapid test and the in-house developed PGL-I up-converting phosphor (UCP)-LFA).…”

Section: Introductionmentioning

confidence: 99%

Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples

Garg

Sahu

Kar

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Leprosy, a progressive, mutilating and highly stigmatized disease caused by Mycobacterium leprae (ML), continues to prevail in the developing world. This is due to the absence of rapid, specific and sensitive diagnostic tools for its early detection since the disease gets notified only with the advent of physical scarring in patients. This study reports the development of a Loop-mediated isothermal amplification (LAMP) technique for fast, sensitive and specific amplification of 16S rRNA gene of ML DNA for early detection of leprosy in resource-limited areas. Various parameters were optimized to obtain robust and reliable amplification of ML DNA. Blind clinical validation studies were performed which showed that this technique had complete concurrence with conventional techniques. Total absence of amplification of negative control DNA confirmed the specificity of this test. Various visual detection methods viz. colorimetric, turbidity differentiation and bridge flocculation were standardized to establish easy-to-read and rapid diagnosis. This technique eliminates the lack of accuracy and sensitivity in skin smear tests in patients and the requirement for expensive lab equipments and trained technicians. The technique holds promise for further expansion and has the potential to cater to the unmet needs of society for a cheap, highly-sensitive and robust rapid diagnosis of ML.

show abstract

“…Well-established procedures are histological diagnosis as well as determination of the bacteriological index (BI, representing the quantitative bacillary load) and the morphological index (MI, representing the percentage of intact solid stained and presumably viable bacilli) by means of examination of Ziehl-Neelsen stained slit skin smears (SSS). Furthermore, phenolic glycolipid I serology is considered an excellent surrogate marker for the bacterial load and can aid in clinical management such as patient classification and monitoring of treatment [3–10].…”

Section: Introductionmentioning

confidence: 99%

Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples

Beißner¹,

Woestemeier²,

Saar³

et al. 2019

BMC Infect Dis

View full text Add to dashboard Cite

Background Leprosy continues to be a health problem in endemic areas. More than 200,000 new cases of leprosy per year suggest that transmission of the disease is still ongoing, presumably as airborne infection through nasal droplets. Late diagnosis supports continued transmission and increases the individual risk for functional disabilities. Laboratory tools are considered beneficial to facilitate early detection and clinical assessment of cases. The aim of this study was to validate molecular tools allowing detection, quantification and assessment of viability of M. leprae from nasal swab samples which are easy to obtain without the need of any invasive procedures. Methods Validation of two real-time PCRs detecting M. leprae DNA (RLEP qPCR) and RNA (16S rRNA RT qPCR) was conducted on “must not detect”/“must detect” samples and 160 pre-treatment nasal swab samples from 20 clinically diagnosed multibacillary (MB) leprosy patients from Togo. Results Both assays were 100% M. leprae specific and showed analytical sensitivities of three templates each. Out of 20 clinically diagnosed MB leprosy patients, 15 (75.0%) had a positive RLEP qPCR result from nasal swab samples. The 16S rRNA RT qPCR detected viable bacilli in nasal swab samples of ten out of these 15 RLEP positive patients (66.7%). Conclusion The combined RLEP/16S rRNA (RT) qPCR assay provides a sensitive and specific tool to determine the bacterial load and viability of M. leprae from nasal swab samples and is applicable for early diagnosis, monitoring treatment response and investigating the role of nasal carriage of M. leprae in human-to-human transmission through aerosol infection. Electronic supplementary material The online version of this article (10.1186/s12879-019-4349-9) contains supplementary material, which is available to authorized users.

show abstract

Integrative literature review of the reported uses of serological tests in leprosy management

Cited by 15 publications

References 32 publications

Avaliação do desempenho de um teste rápido imunocromatográfico no diagnóstico de hanseníase em uma região endêmica no norte do Brasil

Avaliação do desempenho de um teste rápido imunocromatográfico no diagnóstico de hanseníase em uma região endêmica no norte do Brasil

Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples

Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples

Contact Info

Product

Resources

About