Frequency of the toxic shock syndrome toxin-1 gene in methicillin-susceptible and -resistant Staphylococcus aureus isolates from teaching hospitals in Shiraz, Iran

Motamedifar, Mohammad; Ebrahim‐Saraie, Hadi Sedigh; Alfatemi, Seyedeh Mahsan Hoseini; Zalipour, Mehrdad; Kaveh, Mohammad Hossein; Khoshkharam-Roodmajani, Hossein

doi:10.1590/0037-8682-0142-2014

Cited by 17 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The epidemiology of tst -positive MRSA showed regional variations worldwide. A cross-sectional study in Shiraz, Iran has found 18.1% of surveyed MSSA and 11.6% of MRSA possessed tst gene (Motamedifar et al, 2015). Another study from Japan reported that up to 75% of MRSA harbored tst gene, 96.7% of which belonged to agr 2 (Nagao et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Molecular Typing and Variations in Amount of tst Gene Expression of TSST-1-Producing Clinical Staphylococcus aureus Isolates

Zhao¹,

Xu²,

He³

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

The toxic shock syndrome toxin-1 (TSST-1), encoded by tst gene, has been proposed to cause staphylococcal toxic shock syndrome (TSS) in a susceptible host, which highlights the need to evaluate the level of tst gene expression and molecular genetic characteristics of the tst -positive isolates. A total of 916 S. aureus isolates collected from seven hospitals in China were screened for the tst gene. The tst positive isolates were characterized by spa , SCC mec , PFGE, and agr typing. Representative strains were also subjected to MLST typing. qRT-PCR was used to quantify tst and major virulence regulator genes expression. We also sequenced the regions of promoter and open reading frame (ORF) of tst to investigate whether they correlate with the variation in tst expression. We found 208 (22.7%) of surveyed isolates including 198 (29.8%) of MRSA and 10 (4.0%) of MSSA isolates harbored the tst gene. The most common clone among tst positive MRSA isolates belonged to ST5 (CC5)- agr 2-t002-SCC mec II. The amount of tst mRNA varied 8.4-folds among clinical S. aureus isolates. Sequencing the tst promoter revealed a base T deletion in tst high expressed isolates. As for major virulence regulators, srrA, sarT, RNAIII , and ccpA in four tst differentially expressed strains were detected to be highly expressed, respectively. Our study revealed high prevalence of ST5 (CC5)- agr 2-t002-SCC mec II clone among tst positive MRSA in hospitals from China. The levels of tst expression among clinical S. aureus isolates varied, which may be associated with tst promoter and variations in specific virulence regulators.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Typing and Variations in Amount of tst Gene Expression of TSST-1-Producing Clinical Staphylococcus aureus Isolates

Zhao¹,

Xu²,

He³

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…A recent systematic review and meta‐analysis study in Iran notified a relatively high prevalence of tst encoding gene among S aureus clinical isolates (21.3%) with an average range from 0% to 68% 39 . A high prevalence of tst gene among MSSA isolates comparing MRSA isolates was noted by Motamedifar et al 40 …”

Section: Discussionmentioning

confidence: 74%

Genetic analysis of methicillin‐susceptible Staphylococcus aureus clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability

Goudarzi

Sabzehali

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…Variations in S. aureus infection are associated with virulence factors, such as adhesion and exo-protein factors which consist of exotoxins and enzymes, including nuclease, protease, lipase, hyaluronidase, and collagenase. Exotoxins produced by S. aureus are αhaemolysin, β-haemolysin, γ-haemolysin, Panton-Valentine leukocidin (PVL), Toxic Shock Syndrome Toxin-1 (TSST-1), Staphylococcal Enterotoxins (SE) and Exfoliative Toxins (EF) (Bien et al 2011, Motamedifar et al 2015, Oliveira et al 2018. Toxic Shock Syndrome Toxin (TSST-1) is an example of the SAgs exotoxin group that has the ability of pyrogenicity, super-antigenicity and crossing the surface mucosa.…”

Section: Introductionmentioning

confidence: 99%

“…Toxic Shock Syndrome Toxin-1 (TSST-1) has the ability to activate T lymphocytes through a direct bond between the T Cell Receptor (TCR) Vβ with the Major Histocompatibility Complex class II (MHC II) molecules. The activation results on the production of pro-inflammatory cytokines, including Interleukin (IL-2), Interferon (IFN) and Tumor Necrosis Factor (TNF) causing symptoms of high fever, reddish rash, desquamation, hypotension, and acute, life-threatening multi organ failure of which are the symptoms of TSS (Bien et al 2011, Motamedifar et al 2015, Oliveira et al 2018. The ability of bacteria to cause infection depends on the number of virulence factors from bacterial colonisation.…”

Section: Introductionmentioning

confidence: 99%

Molecular Genotyping of Tsst-1 Gene Staphylococcus Aureus from Clinical Specimen

Puspitarini

Kuntaman

Wasito³

et al. 2021

FMI

View full text Add to dashboard Cite

Staphylococcus aureus (S. aureus) is the most common pathogen in humans that causes various infections. Variations in infections due to S. aureus are related to the presence of virulence factors, one of which is Toxic Shock Syndrome Toxin-1 (TSST-1) which is the main cause of Toxic Shock Syndrome (TSS). This study aimed to explore the genetic pattern of the tsst-1 genes among MSSA and MRSA from clinical isolates of patients at Dr. Soetomo Academic Hospital, Surabaya-Indonesia. PCR examination was performed on 106 clinical samples of S. aureus isolates to determine the presence of the tsst-1 genes; subsequently, phylogenetic analysis was performed. The positive tsst-1 genes were found in 3 (5.7%) MSSA isolates and 2 (3.8%) MRSA isolates from 106 samples, of which were divided into 53 MSSA isolates and 53 MRSA isolates. No significant difference was noticed between the tsst-1 genes on both the MSSA and MRSA bacteria (p = 0.648). The positive tsst-1 genes were found in 1 (4.5%) of pus specimens and 2 (16.7%) of blood specimens from MSSA isolates. However, the positive tsst-1 genes were found in neither sputum nor urine specimens. The genes were found in 2 (16.7%) of blood specimens from MRSA isolates. Based on the phylogenetic tree, Indonesian tsst-1 isolates belonged to the same clade as Japan, Iran, Iraq, India, Egypt and the United Kingdom isolates. The prevalence of tsst-1 genes of both MSSA and MRSA from clinical isolates in Dr. Soetomo Academic Hospital Surabaya are 5.7% and 3.8%, respectively. The tsst-1 genes of Surabaya-Indonesia, Japan, Iran, Iraq, India, Egypt and the United Kingdom belonged to the same clade.

show abstract

Frequency of the toxic shock syndrome toxin-1 gene in methicillin-susceptible and -resistant Staphylococcus aureus isolates from teaching hospitals in Shiraz, Iran

Abstract: These results reveal the remarkable risk of S. aureus infections in hospitals, regardless of methicillin-resistance status.

Cited by 17 publications

References 14 publications

Molecular Typing and Variations in Amount of tst Gene Expression of TSST-1-Producing Clinical Staphylococcus aureus Isolates

Molecular Typing and Variations in Amount of tst Gene Expression of TSST-1-Producing Clinical Staphylococcus aureus Isolates

Genetic analysis of methicillin‐susceptible Staphylococcus aureus clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability

Molecular Genotyping of Tsst-1 Gene Staphylococcus Aureus from Clinical Specimen

Contact Info

Product

Resources

About