Genetic analysis of methicillin‐susceptible <i>Staphylococcus aureus</i> clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability

Goudarzi, Hossein; Goudarzi, Mehdi; Sabzehali, Fattaneh; Fazeli, Maryam; Chirani, Alireza Salimi

doi:10.1002/jcla.23494

Cited by 6 publications

(5 citation statements)

References 46 publications

(202 reference statements)

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“…Notably, all ST239 MRSA isolates harbored cna in contrast to the ST239 MSSA isolates; however, cna was also found in MSSA isolates such as ST1, ST96, and ST188. In a previous study, Zamani et al [33] reported that ST239 MRSA clinical isolates collected between 2018 and 2019 carried cna, while ST239 MSSA isolates from the same institution reported by Goudarzi et al [34] contained no cna gene. However, Zamani et al reported that all ST239 MRSA clinical isolates carried clfA, clfB, fnbA, and fnbB, which differed from our results where ST239 MRSA isolates harbored eno, clfA, clfB, and fib.…”

Section: Discussionmentioning

confidence: 95%

Molecular Characteristics and Distribution of Virulence Genes among Staphylococcus aureus Complex Isolates Derived from Vascular Access Infections

Wong

Tseng

Huang

et al. 2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Staphylococcus aureus is a major human pathogen that produces various virulence factors which promote the binding of bacteria to tissues and medical devices such as vascular access devices, thereby developing a wide range of invasive infections. Vascular access serves as an entry site for S. aureus and elevates the risk of infection in the hemodialysis population. Nevertheless, the distribution of virulence genes in Staphylococcus spp. associated with vascular access infections (VAIs) has not been studied previously. In this study, we determined the relationship between the molecular characteristics and virulence profiles of S. aureus isolates obtained from VAIs. We collected isolates from patients with VAIs between August 2017 and December 2020 and further analyzed the molecular characteristics, antimicrobial resistance profiles, and virulence gene distribution in the isolates. Overall, 15 sequence types (STs), including a new ST (ST6892) and 19 spa types, were identified among the 56 isolates. Of the 53 S. aureus isolates, ST8, ST239, ST45, and ST59 were the predominant STs, whereas ST2250 was the only ST in 3 S. argenteus isolates. ST45-SCCmecIV-t026 (abbreviated as ST45-IV-t026), ST59-V-t437, and ST8-IV-t008 were the predominant clones that belonged to agr type I. All isolates harbored clfB and eno, whereas all S. aureus isolates harbored clfA. In addition, 10 Panton-Valentine leucocidin-positive isolates belonged to ST8 and ST59, with ST8-IV-t008 and ST59-V-t437 being the predominant clones. In brief, the distribution of virulence genes associated with STs may assist in the spread of molecular types of Staphylococcus spp.

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Section: Discussionmentioning

confidence: 95%

Molecular Characteristics and Distribution of Virulence Genes among Staphylococcus aureus Complex Isolates Derived from Vascular Access Infections

Wong

Tseng

Huang

et al. 2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…The CC8 clone is described to be a prevalent iMLSB phenotype MRSA in Iran (11). In addition, Goudarzi et al reported that isolates with iMLSB phenotype were observed in CC88/ST239 (13.3%), CC/ST22 (4%), and CC/ST30 (4%) clonal lineages (12).…”

Section: Discussionmentioning

confidence: 99%

“…After the genomic DNA extraction using the phenol-chloroform technique, isolates were screened for the existence of the toxin genes, including pvl, eta, etb, and tst, by PCR (10,12). The mupA, fusA, mecC, and mecA genes were detected by PCR as described elsewhere (4).…”

Section: Detection Of Resistance and Toxin-encoding Determinantsmentioning

confidence: 99%

First Report of the Emergence of mecC Gene and CC8/ST239 Tigecycline-Resistant Staphylococcus aureus Clonal Lineage Isolated from Chronic Suppurative Otitis Media

Amini,

Rahmani,

Hosseini

et al. 2023

Arch Clin Infect Dis

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Background: Staphylococcus aureus is one of the most significant bacteria involved in ear infections. However, insights into the molecular attributes of S. aureus collected from patients with chronic otitis media have yet to be reported in Iran. Objectives: The objective of this study was to assess the molecular characteristics of S. aureus isolated from patients with chronic otitis media. Methods: A total of 55 S. aureus strains retrieved from patients with chronic otitis media were analyzed by the disk diffusion method and polymerase chain reaction (PCR) to identify the nucA gene. Isolates were genetically classified using the coagulase typing method. S. aureus protein A (spa) typing, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing (MLST) were performed on isolates with resistance to specific antibiotics. Results: Overall, out of 55 S. aureus isolates, resistance to mupirocin, fusidic acid, and tigecycline was identified in 12.7%, 5.4%, and 3.6% of isolates, respectively. Fusidic acid-resistant isolates belonged to ST5-SCCmecII/t002/coaII. Two tigecycline-resistant isolates belonged to CC8/ST239-SCCmecIII/t234/coaVIII. One positive mecC isolate belonged to the CC/ST130-SCCmecXI/t843/coaIII clone. Isolates with the iMLSB phenotype belonged to CC/ST80-SCCmecIV/t044/coaII (4 isolates), CC8/ST239-SCCmecIII/t388/coaVI (3 isolates), and CC8/ST8-SCCmecIV/t008/coaIII (1 isolate). Conclusions: Our results indicated that S. aureus isolated from patients with chronic otitis media possesses a unique molecular profile with a high percentage of resistance to multiple medications. These findings suggest that resuming the molecular analysis to improve the control and prevention of ear infections related to S. aureus is necessary.

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“…MSSA-ST30 is an ancestral strain of an epidemic MRSA clone which evolved into MSSA, and is also known as the Southwest Pacific clone [ 60 ]. ST30 is an international successful clone since it has been found in Australia, Europe and Asia [ 61 ]. This lineage is primarily associated with humans, but it has been also found among animals and in the environment [ 55 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs

et al. 2022

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Several studies have showed that a dog-to-human transmission of Staphylococcus aureus occurs. Hunting dogs do not have as much contact with their owners as dogs that live in the same household as the owners; however, these dogs have contact with their owners during hunting activities as well as when hunting game; therefore, we aimed to isolate S. aureus from hunters and their hunting dogs to investigate a possible S. aureus transmission. Nose and mouth samples were collected from 30 hunters and their 78 hunting dogs for staphylococcal isolation. The species identification was performed using MALDI-TOF. The antimicrobial susceptibility profiles were accessed using the Kirby–Bauer method and respective antimicrobial resistance genes were investigated by PCR. Multilocus sequence typing (MLST) and spa- and agr-typing was performed in all S. aureus isolates. S. aureus were detected in 10 (30%) human samples and in 11 (15.4%) dog samples of which 11 and 5 were methicillin-resistant S. aureus (MRSA). Other staphylococci were identified, particularly, S. pseudintermedius. Most S. aureus isolates were resistant to penicillin, erythromycin, and tetracycline. Evidence of a possible transmission of S. aureus between human and dogs was detected in three hunters and their dogs. S. aureus isolates were ascribed to 10 STs and 9 spa-types. A moderate colonization of S. aureus in hunting dogs and their owners was detected in this study. A few dog-to-dog and dog-to-human possible transmissions were identified.

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Genetic analysis of methicillin‐susceptible Staphylococcus aureus clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 6 publications

References 46 publications

Molecular Characteristics and Distribution of Virulence Genes among Staphylococcus aureus Complex Isolates Derived from Vascular Access Infections

Molecular Characteristics and Distribution of Virulence Genes among Staphylococcus aureus Complex Isolates Derived from Vascular Access Infections

First Report of the Emergence of mecC Gene and CC8/ST239 Tigecycline-Resistant Staphylococcus aureus Clonal Lineage Isolated from Chronic Suppurative Otitis Media

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs

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