Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil

Freitas, Yannara Barbosa Nogueira; Souza, Celeste da Silva Freitas de; Magalhães, Jamille Maia e; Sousa, Maressa Laíse Reginaldo de; d’Escoffier, Luiz Ney; Valle, Tânia Zaverucha do; Gonçalves, Teresa Cristina Monte; Gil-Santana, Hélcio R.; Kazimoto, Thais Aaparecida; Ámora, Sthenia Santos Albano

doi:10.1590/0037-8682-0088-2017

Cited by 17 publications

(10 citation statements)

References 46 publications

(56 reference statements)

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“…This is due to our proposal of maintaining a high value of specificity for not to diagnose false positive animals, a necessary property for a serodiagnostic test. Before moving to the field, the specificity in the recognition of recombinant proteins should be analyzed using sera from animals infected by other pathogens that coexist with Leishmania (Maggi and Kramer, 2019) including the study of cross-reactivity with dogs infected with T. cruzi (Roque et al, 2013; Freitas et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence

Ramírez

Moura

Mateus

et al. 2020

Parasite Epidemiology and Control

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Serodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis from geographical areas of Brazil in which the infection is endemic to regions in which the disease is emerging is occurring. This fact makes necessary the analysis of the serodiagnostic capabilities of different leishmanial preparations in distinct geographical locations. In this article sera from dogs infected with Leishmania and showing the clinical form of the disease, were collected in three distinct Brazilian States and were tested against soluble leishmanial antigens or seven parasite individual antigens produced as recombinant proteins. We show that the recognition of soluble leishmanial antigens by sera from these animals was influenced by the geographical location of the infected dogs. Efficacy of the diagnosis based on this crude parasite preparation was higher in newly endemic regions when compared with areas of high disease endemicity. We also show that the use of three of the recombinant proteins, namely parasite surface kinetoplastid membrane protein of 11 kDa (KMP-11), and two members of the P protein family (P2a and P0), can improve the degree of sensitivity without adversely affecting the specificity of the diagnostic assays for canine leishmaniasis, independently of the geographical area of residence. In addition, sera from dogs clinically healthy but infected were also assayed with some of the antigen preparations. We demonstrate that the use of these proteins can help to the serodiagnosis of Leishmania infected animals with subclinical infections. Finally, we propose a diagnostic protocol using a combination of KMP-11, P2a y P0, together with total leishmanial extracts.

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Section: Discussionmentioning

confidence: 99%

Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence

Ramírez

Moura

Mateus

et al. 2020

Parasite Epidemiology and Control

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“…The most affected countries in South America are Brazil (174,194.22 DALYs), followed by Venezuela (27,037.40 DALYs), and Argentina (23,552.58 DALYs); Uruguay being the least affected (350.72 DALYs) based on the estimations of the Global Burden of Diseases [41]. Concerning Chagas in pets, there were a few studies of seroprevalence of Chagas in dogs in Latin America such as Argentina, Brazil, Chile, Colombia, Ecuador, Mexico, Venezuela but none in Paraguay [42][43][44][45][46][47][48]. Several other mammals are a potential reservoir of Chagas.…”

Section: Discussionmentioning

confidence: 99%

“…We identified 43,193 citations between 2000 and 2019 and excluded 43,018 by title and abstract screening, according to the exclusion criteria. We fully assessed 175 articles, resulting in 55 eligible manuscripts that were included in the qualitative synthesis (Fig 1).…”

Section: Systematic Reviewmentioning

confidence: 99%

The burden of zoonoses in Paraguay: A systematic review

2021

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Introduction Underestimation of zoonoses is exacerbated in low and middle-income countries due mainly to inequalities with serious consequences in healthcare. This is difficult to gauge and reduce the impact of those diseases. Our study focuses on Paraguay, where the livestock industry is one of the major components of the country’s economy. Therefore, the rationale of this study was to develop a case study in Paraguay to estimate the dual impact of zoonotic diseases on both the human health and animal health sector and thus determine the societal burden of such diseases. Methodology/Principal findings We conducted a systemic review (including a meta-analysis) to assess the burden of zoonoses in Paraguay, including official reports and grey literature of disease incidence and prevalence. We estimated the Disability Adjusted Life Years (DALYs) and Zoonosis Disability Adjusted Life Years (zDALYs) to measure the difference between the current health status and the desired health situation of animals and the Paraguayan population based on 50 zoonotic diseases suggested by the WHO (World Health Organization), OIE (World Organization for Animal Health) and the National Health in Paraguay. The total DALYs represent 19,384 (95% CI: from 15,805 to 29,733), and zDALYs, 62,178 (95% CI: from 48,696 to 77,188). According to the results, the priority pathogens for DALYs are E. coli, Trypanosoma cruzi, Leishmania spp, and Toxoplasma gondii. When we include the additional animal health burden, the most important pathogens are Brucella spp, E. coli, Trypanosoma cruzi, and Fasciola hepatica for zDALYs. Conclusion/Significance This is the first study to integrate DALYs and zDALYs with important clues related to the health status of Paraguay. Through DALYs and zDALYs, our perspective becomes more complete because we consider not only human health but also animal health. This is important for setting priorities in disease control, especially in a society where livestock contribute significantly to the economy and to human well-being.

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“…In Colombia, dogs have been found as an important source of blood for T. maculata [35]. In other areas, such as the USA (Texas and Louisiana) and Brazil, the T. cruzi seroprevalence of dogs ranged from 7 to 76% [50][51][52][53]. In Northern Argentina, infected dogs constitute a risk factor and have been used as sentinels for T. cruzi domestic transmission [54,55].…”

Section: Diversity Of Mammal Hosts and Its Implication In T Cruzi Trmentioning

confidence: 99%

Triatomine Feeding Profiles and Trypanosoma cruzi Infection, Implications in Domestic and Sylvatic Transmission Cycles in Ecuador

et al. 2021

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Understanding the blood meal patterns of insects that are vectors of diseases is fundamental in unveiling transmission dynamics and developing strategies to impede or decrease human–vector contact. Chagas disease has a complex transmission cycle that implies interactions between vectors, parasites and vertebrate hosts. In Ecuador, limited data on human infection are available; however, the presence of active transmission in endemic areas has been demonstrated. The aim of this study was to determine the diversity of hosts that serve as sources of blood for triatomines in domestic, peridomestic and sylvatic transmission cycles, in two endemic areas of Ecuador (central coastal and southern highland regions). Using conserved primers and DNA extracted from 507 intestinal content samples from five species of triatomines (60 Panstrongylus chinai, 17 Panstrongylus howardi, 1 Panstrongylus rufotuberculatus, 427 Rhodnius ecuadoriensis and 2 Triatoma carrioni) collected from 2006 to 2013, we amplified fragments of the cytb mitochondrial gene. After sequencing, blood meal sources were identified in 416 individuals (146 from central coastal and 270 from southern highland regions), achieving ≥ 95% identity with GenBank sequences (NCBI-BLAST tool). The results showed that humans are the main source of food for triatomines, indicating that human–vector contact is more frequent than previously thought. Although other groups of mammals, such as rodents, are also an available source of blood, birds (particularly chickens) might have a predominant role in the maintenance of triatomines in these areas. However, the diversity of sources of blood found might indicate a preference driven by triatomine species. Moreover, the presence of more than one source of blood in triatomines collected in the same place indicated that dispersal of vectors occurs regardless the availability of food. Dispersal capacity of triatomines needs to be evaluated to propose an effective strategy that limits human–vector contact and, in consequence, to decrease the risk of T. cruzi transmission.

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Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil

Abstract: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission.

Cited by 17 publications

References 46 publications

Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence

Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence

The burden of zoonoses in Paraguay: A systematic review

Triatomine Feeding Profiles and Trypanosoma cruzi Infection, Implications in Domestic and Sylvatic Transmission Cycles in Ecuador

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