Finding of B cell epitope within polyprotein of Japanese encephalitis virus: a clue for development of new Japanese encephalitits vaccine

Yasri, Sora; Wiwanitkit, Viroj

doi:10.1590/0004-282x20140038

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…A previous study proved that the transmembrane domain of E protein displays either low B cell antigenicity or antigenically inert and no B cell epitope was identified in this region [ 32 , 33 ]; however, these studies were inconsistent with another research that transmembrane domain of Japanese encephalitis viruses contains a highly reactive B cell epitope [ 34 ]. In this study, epitopes P27 and P28 located in transmembrane domain of E protein and elicited neutralizing antibody against TMUV.…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of B-cell epitopes within envelope protein of tembusu virus

Zhao

Han

Huang

et al. 2018

Virol J

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BackgroundTembusu virus is a newly emerging flavivirus that caused egg-drop syndrome in ducks in China. TMUV envelope protein is a major structural protein locates at the surface of tembusu virus particle. During tembusu virus infection, envelope protein plays a pivotal role in induction of neutralizing antibody. However, B cell epitopes within envelope protein have not been well studied.MethodA series of 13 peptides derived from E protein of tembusu virus were synthesized and screened by Dot blot with tembusu virus-positive duck serum. Potential B-cell epitopes were respectively fused with GST tag and expressed in E. coli. The immunogenicity and protective efficiency of epitopes were assessed in ducks.ResultsDot blot assay identified the peptides P21 (amino acids 301–329), P23 (amino acids 369–387), P27 (amino acids 464–471) and P28 (amino acids 482–496) as potential B-cell epitopes within the envelope protein of tembusu virus. Immunization of prokaryotically expressed epitopes elicited specific antibodies in ducks and the specific antibody elicited by P21, P27 and P28 could neutralized tembusu virus. In addition, protective test suggested that P21 and P27 could completely protect immunized ducks from TMUV challenge.ConclusionFour potential B cell epiotpes within tembusu virus envelope protein were identified and analyzed in vitro and in vivo. It was demonstrated that two of them (P21 and P27) could elicit neutralizing antibodies in ducks and offer complete protection against tembusu virus challenge. This findings will contribute to the development of epitope vaccine for tembusu virus prevention.

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Section: Discussionmentioning

confidence: 99%

Screening and identification of B-cell epitopes within envelope protein of tembusu virus

Zhao

Han

Huang

et al. 2018

Virol J

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“…An important focus proposed by the vaccine experts is to find one vaccine that can promote B cell activity. The technique to be used is ABCpred Prediction7 (Yasri and Wiwanitkit, 2014). This area mainly targets the immunogenic epitope within the polyprotein of Japanese encephalitis virus.…”

Section: Experimental Vaccinesmentioning

confidence: 99%

Current Scenario of Vaccines for Japanese Encephalitis

Cherukommu¹,

Swamy²,

Saxena³

2014

American Journal of Virology

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Currently various countries in South-East Asia, including India, are facing disaster due to Acute Encephalitis Syndrome (AES), which is primarily caused by Japanese Encephalitis Virus (JEV). A dirty environment and a lack of awareness about the risks associated with mosquito bites may be one of the reasons of the outbreak. Japanese encephalitis is a lethal neuroviral infection for which there is no treatment and it largely affects children less than 14 years. Most of the cases either remain asymptomatic or shortlived mild symptoms with fever. In less than one case in every 300-500, the virus causes serious symptoms, such as seizures, confusion and paralysis. Up to one in every three people who develop serious symptoms die and those who do survive may suffer permanent neurological damage. However, the disease can be prevented by JE-specific vaccination. The current article tries to create awareness and is a compilation of advances made so far for vaccines for Japanese encephalitis.

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B cell epitopes within VP40 of Ebola virus: the clue for vaccine development

Wiwanitkit

2014

Asian Pacific Journal of Tropical Disease

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Finding of B cell epitope within polyprotein of Japanese encephalitis virus: a clue for development of new Japanese encephalitits vaccine

Cited by 3 publications

References 4 publications

Screening and identification of B-cell epitopes within envelope protein of tembusu virus

Screening and identification of B-cell epitopes within envelope protein of tembusu virus

Current Scenario of Vaccines for Japanese Encephalitis

B cell epitopes within VP40 of Ebola virus: the clue for vaccine development

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