Screening and identification of B-cell epitopes within envelope protein of tembusu virus

Zhao, Dong; Han, Kyung-Nam; Huang, Xinmei; Zhang, Lijiao; Wang, Huili; Liu, Na; Tian, Yujie; Liu, Qingtao; Yang, Jing; Liu, Yuzhuo; Li, Yin

doi:10.1186/s12985-018-1052-1

Cited by 18 publications

(9 citation statements)

References 40 publications

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“…Tembusu virus (TMUV) is a newly emerging flavivirus that causes rapid egg drop and neurological symptoms in ducks. Potential in silico predicted B-cell epitopes of the E protein of TMUV fused with glutathione S-transferases tag have been successfully expressed in Escherichia coli [ 89 ]. The findings suggested that two of the four predicted B-cell epitopes could elicit the generation of neutralizing antibodies in ducks and provide protection when challenged with TMUV [ 89 ].…”

Section: Discussionmentioning

confidence: 99%

“…Potential in silico predicted B-cell epitopes of the E protein of TMUV fused with glutathione S-transferases tag have been successfully expressed in Escherichia coli [ 89 ]. The findings suggested that two of the four predicted B-cell epitopes could elicit the generation of neutralizing antibodies in ducks and provide protection when challenged with TMUV [ 89 ]. A chimeric recombinant antigen comprising of predicted CD4+ and CD8+ T cell-specific epitopes derived from Leishmania infantum was successfully expressed in E .…”

Section: Discussionmentioning

confidence: 99%

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Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins

et al. 2021

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All approved coronavirus disease 2019 (COVID-19) vaccines in current use are safe, effective, and reduce the risk of severe illness. Although data on the immunological presentation of patients with COVID-19 is limited, increasing experimental evidence supports the significant contribution of B and T cells towards the resolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Despite the availability of several COVID-19 vaccines with high efficacy, more effective vaccines are still needed to protect against the new variants of SARS-CoV-2. Employing a comprehensive immunoinformatic prediction algorithm and leveraging the genetic closeness with SARS-CoV, we have predicted potential immune epitopes in the structural proteins of SARS-CoV-2. The S and N proteins of SARS-CoV-2 and SARS-CoVs are main targets of antibody detection and have motivated us to design four multi-epitope vaccines which were based on our predicted B- and T-cell epitopes of SARS-CoV-2 structural proteins. The cardinal epitopes selected for the vaccine constructs are predicted to possess antigenic, non-allergenic, and cytokine-inducing properties. Additionally, some of the predicted epitopes have been experimentally validated in published papers. Furthermore, we used the C-ImmSim server to predict effective immune responses induced by the epitope-based vaccines. Taken together, the immune epitopes predicted in this study provide a platform for future experimental validations which may facilitate the development of effective vaccine candidates and epitope-based serological diagnostic assays.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins

et al. 2021

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“…Duck anti-TMUV serum was prepared and stored in our laboratory. The neutralizing antibody titre of the antiserum was assessed by the plaque reduction neutralization test as previously described (Zhao et al, 2018a). Duck peripheral blood lymphocytes were isolated using a duck peripheral blood lymphocyte isolation kit (Haoyang, Tianjin, China) according to the manufacturer's protocol.…”

Section: Ade Inhibition Assaysmentioning

confidence: 99%

Peptide inhibitors of tembusu virus infection derived from the envelope protein

Zhao

Zhang

Han

et al. 2020

Veterinary Microbiology

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The outbreak and spread of Tembusu virus (TMUV) has caused very large losses in the waterfowl-breeding industry since 2010. The viral envelope (E) protein, the principal surface protein of viral particles, plays a vital role in viral entry and fusion. In this study, two peptides derived from domain II (DII) and the stem of the TMUV envelope protein, TP1 and TP2, respectively, were tested for their antiviral activity. TP1 and TP2 inhibited TMUV infection in BHK-21 cells, and their 50% inhibitory concentrations (IC 50 ) were 14.19 mg/L and 7.64 mg/ L, respectively. Viral inhibition assays in different cell lines of avian origin showed that the inhibitory effects of TP1 and TP2 are not cell type dependent. Moreover, TP2 also exhibited inhibitory activity against Japanese encephalitis virus (JEV) infection. The two peptides inhibited antibody-mediated TMUV infection of duck peripheral blood lymphocytes. Co-immunoprecipitation assays and indirect enzyme-linked immunosorbent assays (ELISAs) indicated that both peptides interact with the surface of the TMUV virion. RNase digestion assays confirmed the release of viral RNA following incubation with TP1, while incubation with TP1 or TP2 interfered with the binding between TMUV and cells. Taken together, these results show that TP1 and TP2 may be developed into antiviral treatments against TMUV infection.

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“…Current strategies for epitope identification depend upon detection of epitopes within an individual full-length protein, a low-throughput approach that requires prior knowledge of the antigenic protein, its sequence, and its conformational structure. Technologies to screen for epitopes at the whole proteome level have been developed (e.g., proteomic microarrays, phage and yeast display); however, these technologies require extensive use of synthetic biology and other time-consuming methodologies (e.g., library construction, peptide/protein array preparation, heterologous protein expression) (3,(5)(6)(7)(8)(9)(10)(11). Another major disadvantage of display technologies and use of non-native expression systems is that these methods do not reliably replicate the native properties of the antigenic proteins, including their post-translational modifications, which can lead to inaccurate identification of epitopes.…”

Section: Introductionmentioning

confidence: 99%

Shotgun Immunoproteomic Approach for the Discovery of Linear B-Cell Epitopes in Biothreat Agents Francisella tularensis and Burkholderia pseudomallei

et al. 2021

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Peptide-based subunit vaccines are coming to the forefront of current vaccine approaches, with safety and cost-effective production among their top advantages. Peptide vaccine formulations consist of multiple synthetic linear epitopes that together trigger desired immune responses that can result in robust immune memory. The advantages of linear compared to conformational epitopes are their simple structure, ease of synthesis, and ability to stimulate immune responses by means that do not require complex 3D conformation. Prediction of linear epitopes through use of computational tools is fast and cost-effective, but typically of low accuracy, necessitating extensive experimentation to verify results. On the other hand, identification of linear epitopes through experimental screening has been an inefficient process that requires thorough characterization of previously identified full-length protein antigens, or laborious techniques involving genetic manipulation of organisms. In this study, we apply a newly developed generalizable screening method that enables efficient identification of B-cell epitopes in the proteomes of pathogenic bacteria. As a test case, we used this method to identify epitopes in the proteome of Francisella tularensis (Ft), a Select Agent with a well-characterized immunoproteome. Our screen identified many peptides that map to known antigens, including verified and predicted outer membrane proteins and extracellular proteins, validating the utility of this approach. We then used the method to identify seroreactive peptides in the less characterized immunoproteome of Select Agent Burkholderia pseudomallei (Bp). This screen revealed known Bp antigens as well as proteins that have not been previously identified as antigens. Although B-cell epitope prediction tools Bepipred 2.0 and iBCE-EL classified many of our seroreactive peptides as epitopes, they did not score them significantly higher than the non-reactive tryptic peptides in our study, nor did they assign higher scores to seroreactive peptides from known Ft or Bp antigens, highlighting the need for experimental data instead of relying on computational epitope predictions alone. The present workflow is easily adaptable to detecting peptide targets relevant to the immune systems of other mammalian species, including humans (depending upon the availability of convalescent sera from patients), and could aid in accelerating the discovery of B-cell epitopes and development of vaccines to counter emerging biological threats.

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Screening and identification of B-cell epitopes within envelope protein of tembusu virus

Cited by 18 publications

References 40 publications

Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins

Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins

Peptide inhibitors of tembusu virus infection derived from the envelope protein

Shotgun Immunoproteomic Approach for the Discovery of Linear B-Cell Epitopes in Biothreat Agents Francisella tularensis and Burkholderia pseudomallei

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