Cost-effective fibrinolytic enzyme production by microalga Dunaliella tertiolecta using medium supplemented with corn steep liquor

Silva, Túlio Alexandre Freire; Silva, Páblo Eugênio da Costa e; Nascimento, Thiago Pajeú; Costa, Romero Marcos Pedrosa Brandão; Converti, Attílio; Porto, Ana Lúcia Figueiredo; Bezerra, Raquel Pedrosa

doi:10.1590/0001-3765202320220552

Cited by 3 publications

(7 citation statements)

References 61 publications

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“…However, although the 0-40% ammonium sulfate fraction exhibited the highest fibrinolytic activity (625 ± 20.2 U•mg −1 ), the acetone fraction showed better performance due to its potential fibrinolytic activity (567 ± 49.3 U•mg −1 ) and the highest enzyme yield of 80.3%. These results are similar to those reported by Barros et al [8] and Silva et al [9] for the fibrinolytic enzymes from A. platensis and D. tertiolecta, which showed fibrinolytic activity of 256 U•mg −1 (yield = 53.8%) and 435 U•mg −1 (yield = 37%), respectively.…”

Section: Effect Of Precipitation Methods On the Enzymatic Activitiessupporting

confidence: 92%

“…Similar results were observed in the T. obliquus extracts from the mixotrophic cultures using 0.25% CSL, which also showed higher protease (12.5 ± 2.94 U•mg −1 ) and fibrinolytic (391 ± 40.0 U•mg −1 ) activities using the homogenization method when compared to the sonication methods. These data were similar to those obtained by Silva et al [9] which showed that the fibrinolytic activity from D. tertiolecta was 819 and 422 U•mg −1 using homogenization and sonication, respectively. This can be explained by the possible enzyme denaturation caused by the prolonged sonication time, high temperature, or elevated frequency, as reported by Sukor et al [37] and Ranjha et al [38].…”

Section: Effect Of Extraction Methods On the Enzymatic Activitiessupporting

confidence: 91%

“…In the CSL concentration higher than 0.50%, no T. obliquus cell growth was observed. Similar results were observed in the mixotrophic cultivation of A. platensis and D. tertiolecta using CSL concentrations above 0.6% and 1.0%, which inhibited cell growth [8,9].…”

Section: Cell Growth Profile and Kinetic Parameters Of T Obliquus Cul...supporting

confidence: 82%

“…In this sense, fibrinolytic enzymes from photosynthetic microorganisms such as Chlorella vulgaris, Arthrospira platensis, and Dunaliella tertiolecta have shown promising antithrombotic effects [7][8][9]. However, up to now, there is no report on the fibrinolytic potential of the Tetradesmus genus, although some bioactive compounds such as lectin, linoleic acid, and flavonoids with anticancer and antimicrobial activities have already been reported in the literature [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Corn steep liquor (CSL) is a by-product from the corn wetmilling industries and has high amounts of carbohydrates, amino acids, vitamins, organic acids, and minerals, being a nitrogen-rich source used for the microalgal cultivation [19]. By the way, this by-product has been successful in the production of fibrinolytic enzymes from C. vulgaris, A. platensis, and D. tertiolecta [7][8][9]. So, this study aims to evaluate and compare the biomass and fibrinolytic enzyme productions from T. obliquus cultivated under autotrophic and mixotrophic (using CSL) growth conditions.…”

Section: Introductionmentioning

confidence: 99%

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Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?

Moura,

De Souza,

Da Costa e Silva

et al. 2023

Phycology

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Thrombosis is characterized by the pathological formation of fibrin clots within a blood vessel, leading to the obstruction of blood flow. Fibrinolytic enzymes from microorganisms have been shown to be more efficient and safer in dissolving clots. Then, this study aimed to evaluate the cell growth and fibrinolytic enzyme production of Tetradesmus obliquus under different cultivation conditions. T. obliquus grew under autotrophic and mixotrophic conditions using different concentrations of corn steep liquor (0.25 ≤ CSL ≤ 4.00%). The cells were concentrated and lysed via two different methods (sonication or homogenization) to trigger the release of the enzyme. It was precipitated via acetone or ammonium sulfate additions and purified using ion exchange chromatography. The highest biomass productivity (Px = 130 ± 12.8 mg∙L−1day−1), specific growth rate (µmax = 0.17 ± 0.00 day−1), and fibrinolytic activity (391 ± 40.0 U∙mg−1) was achieved on a mixotrophic cultivation at a 0.25% CSL concentration. The results showed that the homogenizing method had better performance in the release of enzyme, and the precipitation with acetone obtained the highest fibrinolytic activity (567 ± 49.3 U∙mg−1). The purified enzyme showed a specific activity of 1221 ± 31 U∙mg−1 and a molecular mass of 97 kDa. So, the fibrinolytic enzyme from T. obliquus had higher activity when compared to the other fibrinolytic enzymes, being a potential source for the development of therapeutic agents in thrombosis treatment. Additional studies are needed to investigate the biochemical properties and biological profile of this enzyme.

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Section: Effect Of Precipitation Methods On the Enzymatic Activitiessupporting

confidence: 92%

Section: Effect Of Extraction Methods On the Enzymatic Activitiessupporting

confidence: 91%

Section: Cell Growth Profile and Kinetic Parameters Of T Obliquus Cul...supporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?

Moura,

De Souza,

Da Costa e Silva

et al. 2023

Phycology

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show abstract

Fibrinolytic enzyme from Arthrospira platensis: Kinetic and thermodynamic investigation

Moura,

da Silva,

Mirizadeh

et al. 2024

Process Biochemistry

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Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?

Moura¹,

Souza²,

Silva³

et al. 2023

Preprint

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Thrombosis is characterized by the pathological formation of fibrin clots within a blood vessel, leading to the obstruction of blood flow. Fibrinolytic enzymes from microorganisms have exhibited promising effects to dissolve clots in a more efficient and safe way. Then, the aim of this study was to evaluate the biomass and fibrinolytic enzyme production of Tetradesmus obliquus under autotrophic and mixotrophic conditions using different concentration of corn steep liquor (CSL). Different extraction and precipitating methods were tested, and the enzyme was purified by ion exchange chromatography. More advantageous culture condition was mixotrophic using 0.25% CSL, showing the highest values of biomass productivity (Px = 169.3 ± 44.36 mg∙L-1day-1) and specific growth rate (µmax = 0.17 ± 0.00 day-1), and significant fibrinolytic production (391.34 ± 40.03 U∙mg-1). Moreover, fibrinolytic activity was higher when extracted by homogenization and precipitated using acetone, which exhibited clear zone of fibrin degradation in the fibrinolytic plate assay. Additionally, the purified enzyme showed specific activity of 1176.90 ± 140.37 U∙mg-1 and molecular weight around 97 kDa. Finally, the enzyme has higher enzymatic activity than various fibrinolytic enzymes, and the obtained enzyme has potential to be developed as a therapeutic agent in thrombosis treatment. Additional studies are need to investigate the biochemical properties and biological profile of this enzyme.

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Cost-effective fibrinolytic enzyme production by microalga Dunaliella tertiolecta using medium supplemented with corn steep liquor

Cited by 3 publications

References 61 publications

Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?

Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?

Fibrinolytic enzyme from Arthrospira platensis: Kinetic and thermodynamic investigation

Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?

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