Production of Endoglucanase by Exiguobacterium mexicanum OB24 Using Waste Melon Peels as Substrate

Baltaci, Mustafa Özkan; Omeroglu, Mehmet Akif; Albayrak, Seyda; Adıgüzel, Gülşah; Adıgüzel, Ahmet

doi:10.1590/0001-3765202220220151

Cited by 3 publications

(1 citation statement)

References 46 publications

(24 reference statements)

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“…Then, whether the plasmid carried the desired gene region was analysed using the EcoRI rapid cut-off enzyme. Samples that gave positive results and had the optimum concentration were sent to Macrogen (Netherlands) for sequence analysis [20]. After the 16S rRNA sequence data were made significant, they were compared (Blast) with the sequences in Eztaxon and GenBank ® , and then GenBank ® numbers were obtained from NCBI (Table 2).…”

Section: Isolation and Identification Of Lactic Acid Bacteriamentioning

confidence: 99%

Isolation Identification and Molecular Characterization of Lactic Acid Bacteria From Raw Milk Samples Collected from Erzurum Region

Doğan,

Çanakçı Adıgüzel

2024

Türk Doğa Ve Fen Dergisi

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Due to its high-water content, milk is an important source of different microbial contents, especially lactic acid bacteria. The aim of this study is to isolate and identify lactic acid bacteria from raw milk samples collected from Erzurum and its surroundings, and to introduce possible new species, or genera, to the taxonomy. For this purpose, DNAs of pure bacterial cultures obtained from 50 raw milk samples collected from producers in Erzurum and its districts were isolated, isolates that differed from each other were selected by rep-PCR, and 11 different species and subspecies [Corynebacterium casei, Enterococcus italicus, E. durans, Lactococcus lactis, Lactococcos lactis subsp. lactis, Lactococcos lactis subsp. hordniae, Lactobacillus paracasei, Leuconostoc lactis, Staphylococcus succinis, Streptococcus parauberis ve S. uberis] in raw milk samples by 16S rRNA sequence analysis. It was concluded that the (GTG)5-PCR method was more successful than BOX-PCR in the identification of lactic acid bacteria isolated from raw milk samples at a species and subspecies level, and the isolate coded TS10, which was 98% similar Streptococcus uberis, may be a new species and should be re-examined with advanced diagnostic techniques.

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Section: Isolation and Identification Of Lactic Acid Bacteriamentioning

confidence: 99%

Isolation Identification and Molecular Characterization of Lactic Acid Bacteria From Raw Milk Samples Collected from Erzurum Region

Doğan,

Çanakçı Adıgüzel

2024

Türk Doğa Ve Fen Dergisi

Self Cite

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Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Baltacı,

Görmez

et al. 2024

Environ Sci Pollut Res

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Polyhydroxyalkanoates have attracted great interest as a suitable alternative to petrochemical based plastics due to their outstanding properties such as biodegradability and biocompatibility. However, the biggest problem in the production of microbial polyhydroxyalkanoates is low cost-effectiveness. In this study, polyhydroxyalkanoate production was carried out using waste substrates with local isolates. Culture conditions were optimized to increase the polyhydroxyalkanoate production potential. The produced polyhydroxyalkanoate was characterized by FTIR analyses, and its metabolic pathway was determined by real-time PCR. According to the results, the best polyhydroxyalkanoate producer bacteria was characterized as Pseudomonas neustonica NGB15. The optimal culture conditions were detected as 30 g/L banana peel powder, 25 °C temperature, pH 8, and 4-day incubation time. Under the optimized conditions, 3.34 g/L PHA production was achieved. As a result of FTIR analyses, major peaks were obtained at 1723, 1277, 1261, 1097, 1054, and 993 cm−1. These peaks represent that the type of produced polyhydroxyalkanoate was poly-β-hydroxybutyrate. According to gene expression profile of NGB15, it was determined that Pseudomonas neustonica NGB15 produces PHA using the de novo fatty acid synthesis metabolic pathway. In conclusion, poly-β-hydroxybutyrate production by Pseudomonas neustonica NGB15 using a low-cost fermentation medium has been shown to be biotechnologically promising.

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Enhanced biodegradation of crude oil contamination by indigenous bacterial consortium under real conditions

Baltaci

Omeroglu

Özkan

et al. 2023

Biocatalysis and Biotransformation

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Production of Endoglucanase by Exiguobacterium mexicanum OB24 Using Waste Melon Peels as Substrate

Cited by 3 publications

References 46 publications

Isolation Identification and Molecular Characterization of Lactic Acid Bacteria From Raw Milk Samples Collected from Erzurum Region

Isolation Identification and Molecular Characterization of Lactic Acid Bacteria From Raw Milk Samples Collected from Erzurum Region

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Enhanced biodegradation of crude oil contamination by indigenous bacterial consortium under real conditions

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