Biotechnological potential of microorganisms from textile effluent: isolation, enzymatic activity and dye discoloration

Abstract

Environmental pollution may be considered one of the main problems affecting the world population. As the effl uents from textile industries are the largest representatives of sources of pollution of water bodies due to the disposal of colored compounds in the environment. Microorganisms capable of thriving in textile wastewater may exhibit metabolic machinery to synthesize a wide variety of enzymes and/or secondary metabolites of industrial interest. The present work investigated the biotechnological potential of fi lamentous fungi from wastewater of a textile industry for the production of laccase, cellulase, amylase and lipase enzymes and their potential for discoloration capacity of Remazol Brilliant Blue R synthetic dye. The isolate Aspergillus sydowii (ITF 30) presented the best cellulase (46.74 U mL -1 ), amylase, lipase and laccase (0.0273 U L -1 ) production, as well as RBBR dye discoloration ability in solid medium, followed by isolate Aspergillus sydowii (ITF 27) able to synthesize cellulase, amylase and laccase and had the capacity to discolor 74.7% of RBBR in liquid medium. The results of the present work encourage future studies of characterization, optimization and purifi cation of the enzymes encountered, aiming to be used in bioremediation processes of textile industrial effl uents.

Eco-Friendly Solution Based on Rosmarinus officinalis Hydro-Alcoholic Extract to Prevent Biodeterioration of Cultural Heritage Objects and Buildings

“…Although not tested in biodeterioration studies, chlorogenic acid, an important compound present in the R. officinalis extract has been shown to inhibit carbohydrates-hydrolyzing enzymes such as amylases and glucosidases being currently investigated as hypoglycaemic adjuvant [ 75 ]. Other phenolic compounds such as p-coumaric acid, vanillic acid, syringing acid, benzoic acid, and ferulic acid are known for their ability to inhibit the production of enzymes such as laccase (that might be involved in textile discoloration), as demonstrated for Botryosphaeria genera [ 76 , 77 ]. Further, it has been shown that caffeic acid, p-coumaric acid, and ferulic inhibit protease, cellulase and esterase production in F. oxysporum, while p-coumaric acid and ferulic acid strongly decrease cellulase activity in the case of T. reesei strains [ 78 , 79 ].…”

Bioremediation potential of industrial laundry effluent by agaricomycetes from brazilian tropical dry forest

“…Normally, the KM should not vary much if it was a pure enzyme, but because it is an enzymatic complex, there was a synergism between the intra and extracellular enzymes present. This synergism allowed the potentiation of the complex (Mattila et al, 2022;Bernal et al, 2021;Sakai et al, 2022), obtaining an almost complete degradation of the ICD in the concentration of 50 ppm in 25 days of experimentation, above 90% in Gi2 and above 80% in Gi1 (Table 2).…”

“…The lignin degradation occurs because of multi-enzymatic process resulting from the coordinated action of a series of intra and extracellular enzymes such as oxidoreductases, as well as other oxidases producing hydrogen peroxide and by intermediate metabolites of low molecular mass (Mattila et al, 2022;Bernal et al, 2021;Sakai et al, 2022).…”