Unraveling the genetic complexity of a cultivated breeding population of “yerba mate” (Ilex paraguariensis St. Hil.)

Abstract

We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic markers indicate that most genetic variation reside within plantations sites (≥ 95%), and that these plantations were established on highly variable materials (either as seeds or plantlets) derived from, at least, 63 maternal lineages. Moreover, our study suggests that the genetic structure within each plantation site was most likely shaped by past admixture favored by farmers´ practices during the establishment of each plantation. Also, subsequent constraints in gene flow and/or a low level of shared polymorphism among plantations could have contributed to current structure.

Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species

“…A subset of those plants (two males and two females) was examined here, yielding a comparable value (I = 0.501 ± 0.225) even though it derives from NML. Another genotyping analysis of dominant markers from I. paraguariensis plants from a breeding population (N = 128) reported a genetic diversity value of 0.569 (Paiva et al 2020). Then, it is reasonable to conclude that, at least for this species, the NML render a decent indicator of genetic diversity.…”

Genetic improvement of Ilex paraguariensis in western Santa Catarina State: estimate of phenotypic and genetic parameters

“…Yerba mate (Ilex paraguariensis A.St.-Hil.) has significant economic, environmental and cultural importance in South America due to its traditional consumption as tea, chimarrão and tererê (Sturion & Resende 2010;Paiva et al 2020). Its natural occurrence area covers parts of Argentina, Brazil, Paraguay and Uruguay (Sturion & Resende, 2010).…”