Bioactivity and action mechanism of green propolis against Pythium aphanidermatum

Abstract

We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.ml-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.

Chemical Structure and Antifungal Activity of Agean Region of Propolis in Türkiye

“…In recent years, the antifungal effect of propolis on different phytopathogenic fungi has been confirmed in many national and worldwide studies (Dinler et al, 2017;Pazin et al, 2019;Gregolin et al, 2019;Kim et al, 2019;Özyiğit, 2020;Abo-Elyousr et al, 2021).…”

“…There are many alternative control studies in which biological origin compounds such as medicinal plants, propolis and biocontrol agents inhibit the in vitro growth of plant pathogenic fungi (Davari and Ezazi, 2022). In recent years, many studies have proven the antimicrobial properties of propolis against bacterial and fungal pathogens (Curifuta et al, 2012;Pazin et al, 2019;Abo-Elyousr et al, 2021;Davari and Ezazi, 2022). Several methods such as biological control, use of aromatic oils, propolis, fungicides and other methods have been investigated to minimize crop losses caused by Fusarium genus and other soil-borne pathogens in cultivated crops (Erdoğan et al, 2014;Erdoğan et al, 2016;Koç et al, 2018, Er, 2021Çakar et al, 2022;Khalil et al, 2022).…”

SÍNTESE DE NANOPARTÍCULAS DE PRATA (Ag) E INCORPORAÇÃO NO FARELO DA CASCA DO PINHÃO

“…Na tentativa de diminuir o impacto dos pesticidas disponíveis atualmente, muitos pesquisadores têm voltado os estudos para compostos bioativos naturais que atuam de forma diferente dos produtos comumente conhecidos (PAZIN et al, 2019), como é o caso da própolis.…”

“…A própolis egípcia reduziu a murcha bacteriana do tomate causada por Ralstonia solanacearum sob condições de casa de vegetação e de campo (ABO-ELYOUSR et al, 2017). O extrato etanólico da própolis verde inibiu o fitopatógeno Pythium aphanidermatum, desta forma o autor afirma que própolis é uma forma eficaz e barata para controlar o impacto do perigoso fitopatógeno na agricultura (PAZIN et al, 2019).…”