Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)

Abstract

Piper betle L., is an evergreen perennial creeper belonging to family Piperaceae and is known to possess numerous medicinal properties. Current study focuses on evaluating antioxidant and antimicrobial potential of betel leaf. For the present study, distilled water, hexane, acetone and ethanolic extracts of two varieties of betel leaves: Meetha paan and Banarasi paan were used. Biochemical tests such as proximate analysis (moisture, ash, protein, lipids, minerals viz., sodium and potassium), antioxidant activity tests (DPPH radical scavenging activity, total phenolics, ascorbic acid, reducing power) and antimicrobial test (antibacterial and antifungal susceptibility test) against four pathogens viz., B. subtilis, E. coli, A. niger and S. cerevisiae were determined. Ethanolic extract had the highest antioxidant activity (89.46% inhibition), while the aqueous extract exhibited lowest antioxidant activity (62.03% inhibition). With increasing concentration (5, 10, 25 and 50 µg/mL), the reducing power of leaf extracts also increased. The ascorbic acid was not significant in Banarasi paan (5.21mg/100 g) and Meetha paan (5.20mg/100 g). The highest antibacterial activity of ethanolic extract (Banarasi paan) may be attributed to the presence of phytosterols in the leaf varieties. Antioxidant and antimicrobial potential study will help to build a database and promote the utilization of betel leaf as a medicinal herb.

Exploration of Multifunctional Properties of Piper Betel Leaves Extract Incorporated Polyvinyl Alcohol-Oxidized Maize Starch Blend Films for Active Packaging Application

“…Similar trends were observed in the results reported by other researchers, suggested that maximum phenolic content revealed the higher radical scavenging activity [66][67].…”

Bio‐actives of betel leaf (Piper betle L.): A comprehensive review on extraction, isolation, characterization, and biological activity

“…Piper betel L. leaf extract were selected to analyze the antibacterial activity against some selected microorganisms that are described in Table 3 and the methanolic extract was found to be more effective to inhibit the microbial strains (Nair & Chanda, 2008) Among the solvent extracts, ethanolic extract showed maximum inhibition zone against E. coli followed by S. aureus (Sarma et al, 2018).…”

“…haldia , leaves extract using chloroform contained 1‐ n ‐dodecanyloxy resorcinol and desmethylenesqualenyl deoxy‐cepharadione‐A (Atiya et al, 2018). Sarma et al (2018) focused on evaluating antioxidant activity of two selected varieties of betel leaves ( Meetha and Banarasi paan ) extract using distilled water, hexane, acetone, and ethanol as solvent. The results revealed that among the extracts, ethanolic extract of Banarasi paan had the highest antioxidant (89.46%).…”

Vertical Alignment of Liquid Crystals on Comb-Like Renewable Chavicol-Modified Polystyrene

“…Chavicol has phenol derivatives with a phenylpropene structure and is extracted from various plants such as Piper betle [ 43 , 44 ]. Piper betle essential oil containing chavicol plays a vital role in antifungal, antibacterial, and antioxidant activities, preventing various diseases [ 44 , 45 , 46 , 47 , 48 ]. For example, chavicol extracted from Piper betle exhibited effective inhibition of bacterial growth against Gram-positive bacteria such as Staphylococcus aureus in conjunctivitis patients [ 45 ].…”