UV induced surface modification on improving the cytocompatibility of metallocene polyethylene

Abstract

Demand for medical implants is rising day by day as the world becomes the place for more diseased and older people. Accordingly, in this research, metallocene polyethylene (mPE), a commonly used polymer was treated with UV rays for improving its biocompatibility. Scanning electron microscopy (SEM) images confirmed the formation of crests and troughs, which depicts the improvement of surface roughness of mPE substrates caused by UV etching. Accordingly, the contact angle measurements revealed that the wettability of mPE-2.5 J/cm2 (68.09º) and mPE-5 J/cm2 (57.93º) samples were found to be increased compared to untreated mPE (86.84º) indicating better hydrophilicity. Further, the UV treated surface exhibited enhanced blood compatibility as determined in APTT (untreated mPE- 55.3 ± 2.5 s, mPE-2.5 J/cm2 - 76.7 ± 4.1 s and mPE-5 J/cm2 - 112.3 ± 2 s) and PT (untreated mPE - 24.7 ± 1.5 s, mPE- 2.5 J/cm2 - 34.3 ± 1.1 s and mPE-5 J/cm2 - 43 ± 2 s) assay. Moreover, the treated mPE-2.5 J/cm2 (4.88%) and mPE-5 J/cm2 (1.79%) showed decreased hemolytic percentage compared to untreated mPE (15.40%) indicating better safety to red blood cells. Interestingly, the changes in physicochemical properties of mPE are directly proportional to the dosage of the UV rays. UV modified mPE surfaces were found to be more compatible as identified through MTT assay, photomicrograph and SEM images of the seeded 3T3 cell population. Hence UV-modified surface of mPE may be successfully exploited for medical implants.

Enhanced mechanical, thermal and biocompatible nature of dual component electrospun nanocomposite for bone tissue engineering

“…The blood compatibility evaluation of the fabricated materials is one of the main factors which decides their role in clinical applications. The developed materials should reduce thrombus formation and less toxic to red blood cells (RBC’s) (Jaganathan & Prasath, 2018). In this study, a novel electrospun bone scaffold based on polyurethane (PU) mixed with CO and NO was fabricated.…”

Engineered multicomponent electrospun nanocomposite scaffolds comprising polyurethane loaded with ghee and propolis for bone tissue repair

“…The CH 2 stretching was observed at sharp bands 2939 cm −1 and 2853 cm −1 , while its vibrations were seen at 1413 cm −1 . The twin peak at 1730 cm −1 and 1703 cm −1 corresponds to CO stretching and its vibrations of alcohol group were observed at 1221 cm −1 , 1104 cm −1 and 770 cm −1 , respectively [20]. From the spectra of PU/ghee and PU/ghee/propolis nanocomposites, no new peak formation was observed, but the peak intensity was decreased in PU/ghee and PU/ghee/propolis nanocomposites indicating the formation of hydrogen bond [25].…”

“…When the blood touches the materials, there might be rapid absorption of plasma proteins which enables the platelet surface interaction. The platelet surface interaction might result in thrombus formation which leads to the failure of the fabricated material [20]. In this article, blood compatibility assessments of electrospun membranes were investigated.…”

Morphological properties of almond oil constituted nanofibrous scaffold for bone tissue engineering

“…In extension, it might lead to platelet surface interaction, which may cause thrombus formation resulting in material failure. 20 In this study, the PU and PU/almond oil membrane was fabricated using electrospinning technique, and their physicochemical characteristics and blood compatibility assessments were studied.…”