Urease activity according to the different inhibitors

Abstract

The objective of this study was to evaluate NBPT, zeolite and humic acid in different concentrations as inhibitors of the activity of the enzyme urease. The activity of the urease enzyme was quantified by the methods proposed by May and Douglas (1976) and Witte and Medina-Escobar (2001). For this reason, two experiments were conducted in a completely randomized design in split plots, in which the incubation periods are the plots and the fertilizers with the inhibitors at the different concentrations are the subplots with three replications. Fifteen fertilizers based on NBPT-coated urea, humic acid and zeolite were used in different concentrations. The two methods of analysis used in the experiment confirmed the efficiency of NBPT in inhibiting the enzymatic action even at low concentration, with 0.02% (m/m) being sufficient to inhibit urease, with no difference between inhibitor concentrations. The addition of humic acid reduced the activity of urease after 36 hours of reaction when the activity of the enzyme was evaluated by the method proposed by May and Douglas (1976). Zeolite did not influence the activity of the urease enzyme when analyzed by the May and Douglas method (1976).

Humic acid complex formation with urea alters its structure and enhances biomass production in hydroponic maize

“…yield, N uptake, and the residual amount of fertilizer-N). 4 HA can be used as a urease inhibitor to delay the decomposition of urea, 8,9 and the functional groups in HA may interact with the thiol group of urease to form a larger particle size of complex to inhibit the activity of urease. 10 In addition, HA can be used as a biostimulant to enhance plant growth and N absorption and assimilation, [11][12][13][14][15] with the stimulatory effect of HA varying with its structure and molecular weight.…”

Valorization of Polyphenols from Stryphnodendron adstringens Bark for Use as a Sustainable Inhibitor of Nitrogen Volatilization in Soil

“…This result was consistent with those in the literature. Recent studies have shown that the increase in NBPT concentration is not related to the increase in efficiency of nitrogen loss reduction and consequently an increase in productivity. , …”

“…Recent studies have shown that the increase in NBPT concentration is not related to the increase in efficiency of nitrogen loss reduction and consequently an increase in productivity. 53,54 The results of NBPT in the literature are scarce due to its thermal instability, which makes it difficult to obtain these data. 8 The study showed that polyphenols from Stryphnodendron adstringens are potent natural urease inhibitors, showing great potential to be used as a substitute for NBPT since several factors limit their use, such as high cost, the lack of yield optimization even with immense rates applied to the soil, and the recent findings related to their toxicity to the plant.…”

Determination of the Optimum Conditions for Urease Inhibittion Extracted From Some Local Plants

“…Eighty grams of peat was extracted with 800 ml of alkaline potassium hydroxide (KOH) at a concentration of 0.1 mol L-1, determining a mass/ volume (m/v) ratio of 1:10 for urease inhibitor extraction. The material was left for a two-hour rest and then centrifuged, extraction was performed at room temperature (25-30°C) (7).…”