Preparation and characterization of alginate and gelatin microcapsules containing Lactobacillus rhamnosus

Abstract

This paper describes the preparation and characterization of alginate beads coated with gelatin and containing Lactobacillus rhamnosus. Capsules were obtained by extrusion method using CaCl 2 as cross linker. An experimental design was performed using alginate and gelatin concentrations as the variables investigated, while the response variable was the concentration of viable cells. Beads were characterized in terms of size, morphology, scanning electron microscopy (SEM), moisture content, Fourier Transform Infrared Spectrometry (FTIR), thermal behavior and cell viability during storage. The results showed that the highest concentration of viable cells (4.2 x 10 9 CFU/g) was obtained for 1 % w/v of alginate and 0.1 % w/v of gelatin. Capsules were predominantly spherical with a rough surface, a narrow size distribution ranging from 1.53 to 1.90 mm and a moisture content of 97.70 ± 0.03 %. Furthermore, FTIR and thermogravimetric analysis indicated an interaction between alginate-gelatin. Cell concentration of alginate/gelatin microcapsules was 10 5 CFU/g after 4 months of storage at 8 o C.

Use of sodium alginate in the preparation of gelatin-based hard capsule shells and their evaluation in vitro

“…46 The peak intensity at $1639 cm À1 is characteristic of the -CONH 2 group, which indicated that the alginate's negative group is in all probability associated with gelatin's positive load. 47 Increased peak intensity at 1630 cm À1 , which corresponded to CONH 2 (C]O), could indicate that the alginate's negative group could be associated with gelatin's positive load. This observation is suggestive of intermolecular interactions between alginate and gelatin, which thus provide conclusive evidence of good molecular compatibility between the two polymers, which will improve its mechanical properties.…”

Spectroscopic and Electrical Properties of Sodium Alginate/TiO2-Carbon Nanotube Composite

“…Besides, bands at 920 cm -1 and 822 cm -1 are attributed to the C-H vibration of the pyranose (ring of the alginate) group [23]. Compared with the spectrum of NaAlg beads and NaAlg treated with CNT/TiO 2 (1:b,c,d), the strong band of NaAlg beads at 1603 cm -1 which assigned to the carboxyl group shifted from 1608 cm -1 to 1627 cm -1 respectively indicating the specified intermolecular hydrogen bonds between the carboxyl group of NaAlg and CNT/TiO 2 [24]. The band at around 1704 cm -1 corresponding to the stretching mode of the carboxylic acid groups of the CNT [25], which is observed at the concentration of 10% of CNT/TiO 2 .…”

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

“…The emulsified microcapsules contain a small particle size and do not affect the taste of the products in which the microcapsules are added. In addition, emulsification is more suitable for large-scale production [ 149 ].…”