Effect of Bauhinia holophylla treatment in Streptozotocin-induced diabetic rats

Pinheiro, Marcelo Salvatte; Rodrigues, Luhara Silva; Neto, Leila Santos; Moraes-Souza, Rafaianne Queiroz; Soares, Thelma Shirlen; Américo, Madileine Francely; Campos, Kleber Eduardo de; Damasceno, Débora Cristina; Volpato, Gustavo Tadeu

doi:10.1590/0001-3765201720160050

Cited by 19 publications

(14 citation statements)

References 24 publications

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“…Despite the increased appetite, insulin deficit reduces all anabolic processes and increases catabolic processes, contributing to further body weight loss [33]. This biological condition was not observed in the present study, in contrast to previous studies using this diabetic model [32,34]. Perhaps the glycemic level, although high, was not sufficient to decrease the weight of these animals during the experimental period.…”

Section: Discussioncontrasting

confidence: 89%

“…Nevertheless, the hyperglycemia and AUC were reduced in the rats with uncontrolled diabetes, confirming the effectiveness of the extract at reducing the blood glucose level. However, the plant did not reduce hyperglycemia to normal levels probably because of the severe diabetes status, which is characterized by severe hyperglycemia (glucose level greater than 300 mg/kg) [32]. Pereira et al [15] verified that the ethanolic H. speciosa extract (300 mg/ kg) exerted a potential anti-diabetic effect related to the inhibition of intestinal α-glucosidase activity and stimulation of glucose uptake by adipocytes.…”

Section: Discussionmentioning

confidence: 99%

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A treatment with a boiled aqueous extract of Hancornia speciosa Gomes leaves improves the metabolic status of streptozotocin-induced diabetic rats

Neto

Moraes-Souza

Soares

et al. 2020

BMC Complement Med Ther

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Background: Hancornia speciosa is usually used in Brazilian folk medicine to treat diabetes. The hypothesis of the present study is that this medicinal plant exerts beneficial effects on hyperglycemia, preventing diabetic complications. Therefore, the aim of this study was to evaluate the treatment effect of the aqueous extract of H. speciosa leaves on metabolic parameters of diabetic rats. Methods: The H. speciosa extract (400 mg/Kg) was administered to both nondiabetic and severely diabetic female Wistar rats by gavage. The Oral Glucose Tolerance Test was performed and the area under the curve (AUC) was estimated on day 17 of pregnancy. After 21 days of treatment, the animals were anesthetized and killed to obtain organ weights. Blood samples were collected for an analysis of serum biochemical parameters. Results: After treatment with the H. speciosa extract, the parameters of nondiabetic rats remained unchanged. In treated diabetic rats, glycemia, AUC, dyslipidemia parameters, and relative organ weights were decreased compared with nontreated diabetic rats. Severely diabetic rats showed decompensated hyperglycemia, polydipsia, hyperphagia and dyslipidemia. However, the aqueous extract of H. speciosa leaves decreased diabetes complications (indicating a lack of toxicity), reduced blood glucose levels, and exerced lipid-lowering effects. Conclusion: Based on or findings, the H. speciosa leaf extract may be a safe and promising candidate treatment for diabetes and other diseases.

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Section: Discussioncontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

A treatment with a boiled aqueous extract of Hancornia speciosa Gomes leaves improves the metabolic status of streptozotocin-induced diabetic rats

Neto

Moraes-Souza

Soares

et al. 2020

BMC Complement Med Ther

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“…citrate buffer. Tail blood glucose concentrations were measured using a One Touch Ultra glucometer (Johnson & Johnson ® ) 7 days after the STZ treatment and glucose concentrations exceeding 300 mg/dL confirmed the presence of diabetes [21]. …”

Section: Methodsmentioning

confidence: 99%

Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes

et al. 2017

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PurposeThe Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes.MethodsDiabetes was induced by streptozotocin (STZ, 40 mg/kg) in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group): non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed.ResultsThe non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI) and coronary artery risk index (CRI), and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group.ConclusionOur results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

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“…2004; Pinheiro et al. 2017). For example, a recent study evaluating the hypoglycaemic potential of B. holophylla , a species used in folk medicine to treat diabetes (Oliveira and Saito 1989), failed to demonstrate antidiabetic activity (Pinheiro et al.…”

Section: Introductionmentioning

confidence: 99%

Hypoglycaemic activity of Bauhinia holophylla through GSK3-β inhibition and glycogenesis activation

Camaforte

Saldanha

Vareda

et al. 2019

Pharmaceutical Biology

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Context: Bauhinia L. species, including Bauhinia holophylla (Bong.) Steud. (Fabaceae), have traditionally been used to treat diabetes. Bauhinia is a complex botanical genus, and the indiscriminate use of the diverse Bauhinia species is reflected in the experimental divergence of their medicinal potential. Objective: The hypoglycaemic and hypolipidaemic effects, molecular mechanism of action and phytochemical properties of an authentic extract of B. holophylla leaves were evaluated. Materials and methods: A phytochemical study of a 70% EtOH extract was performed using FIA-ESI-IT-MS/MS n and HPLC-PAD-ESI-IT-MS. The extract (200 or 400 mg/kg b.w.) was administered for 14 days to streptozotocin-induced diabetic Swiss mice. Glucose tolerance and insulin sensitivity, blood parameters, gene and protein expression, and the in vivo and in vitro inhibition of intestinal glucosidases were assessed. Results: HPLC-PAD-ESI-IT-MS analysis identified flavonoid derivatives of quercetin, myricetin, luteolin and kaempferol. Treatment with 400 mg/kg of the extract reduced blood glucose (269.0 ± 32.4 mg/dL vs. 468.0 ± 32.2 mg/dL for diabetic animals), improved glucose tolerance, decreased cholesterol and triglyceride levels, and increased the mRNA expression of proteins involved in glucogenesis in the liver and muscle, such as PI3-K/Akt, GS, GSK3-β (ser-9), AMPK and Glut4. The activity of intestinal maltase was inhibited in vitro (IC 50 : 43.0 µg/mL for the extract compared to 516.4 µg/mL for acarbose) and in vivo . Discussion and conclusions: Treatment with B. holophylla was associated with a marked hypoglycaemic effect through the stimulation of glycogenesis and inhibition of gluconeogenesis and intestinal glucose absorption, without increasing basal insulinaemia.

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Effect of Bauhinia holophylla treatment in Streptozotocin-induced diabetic rats

Cited by 19 publications

References 24 publications

A treatment with a boiled aqueous extract of Hancornia speciosa Gomes leaves improves the metabolic status of streptozotocin-induced diabetic rats

A treatment with a boiled aqueous extract of Hancornia speciosa Gomes leaves improves the metabolic status of streptozotocin-induced diabetic rats

Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes

Hypoglycaemic activity of Bauhinia holophylla through GSK3-β inhibition and glycogenesis activation

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