Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Lemes, Susy Ricardo; Chaves, Dwight Assis; Silva, Nelson J. Da; Carneiro, Cristiene Costa; Chen-Chen, Lee; Almeida, Luciane Madureira; Gonçalves, Pablo José; Reis, Paulo Roberto de Melo

doi:10.1590/0001-3765201720150797

Cited by 10 publications

(3 citation statements)

References 40 publications

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“…After CP therapy, there were many changes in DNA damage, chromosomal aberrations, and a drop in mitotic index. These findings are consistent with the current research, which found DNA strand damage and a drop in the mitotic index, as well as chromosomal abnormalities such as ring chromatid fragmentation, chromatid deletions, and polyploidy in the C.P.-only group [16] .…”

Section: Discussionsupporting

confidence: 93%

Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

2022

International Journal of Medical Arts

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Article informationBackground: Chemotherapeutic agents are associated with different unwanted effects; the dangerous are mutagenic actions. Researchers do their best to ameliorate such effects by different supportive agents. Objective: The study designed to demonstrate the mutagenicity of the chemotherapeutic drugs especially cyclophosphamide [CP] and possible protective effects of Zinc Chloride [Zn Cl] Materials and Methods: The experiment was designed as 4 groups each group contain 12 animals, the first group [control group] was injected intraperitoneal normal saline [1 ml/kg] every other day for 20 days, the second group was given intraperitoneal Zn Cl [4 mg/kg] every other day for 20 days. The third group [Test group] injected by intraperitoneal cyclophosphamide [200 mg/kg] as a single dose on 15 th day of experiment, Fourth group [protective group] was given intraperitoneal ZnCl [4 mg/kg] every other day for 20 days and injected intraperitoneal single dose by CP [200 mg/kg] on 15 th day.Results: There were significant changes in blood cells, mitotic index and increased frequency of structural and numerical changes in chromosomes compared with the control group and these outcomes attributed to effects of free radicals of CP on health cells. The protective effect of Zn Cl which decrease blood cell changes, the structural and numerical aberrations in relation to the test group. Conclusion:CP has harmful effects on the blood cells and chromosomes. Zinc Chloride has a protective effect against the harmful effects of CP.

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Section: Discussionsupporting

confidence: 93%

Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

2022

International Journal of Medical Arts

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“…The comet assay is a technique used for detecting the presence of single strand breaks of DNA at alkali-sensitive sites or sites of incomplete excision repair in mammalian cells 8 . These tests have been used to analyze the toxicity 9 as well as antigenotoxic, anti-mutagenic and anti-carcinogenic effects 10,11 of extracts and isolated compounds from plants. The genotoxicity of anticancer drugs can result in secondary tumors in non tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Chemoprotective Effect of Carwin Capsules against Cyclophosphamide Induced Genotoxicity by In Vivo Micronucleus Assay

Thakur¹,

Upmanyu

Saxena³

et al. 2022

J. Drug Delivery Ther.

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The protective effects of carwin capsules (CAR) against toxicity induced by cyclophosphamide (CYC) in mice were investigated using the micronucleus assay for anticlastogenic activity in mouse bone marrow cells. The experimental animals were pre-treated orally with the drug suspension (carwin capsule suspension) for seven consecutive days (drug alone in group III and in combination with standard drug in group IV). The standard drug (cyclophosphamide, 50 mg/kg bw) was dissolved in saline and injected intraperitoneally. The results demonstrate that carwin was found to be significant (P<0.05) as compared to cyclophosphamide treated as the no. of MNPCEs in carwin treated was decreased effectively. And also carwin was able to significantly (P<0.01) protect the action caused by cyclophosphamide as well was also found to be very effective antigenotoxic (P<0.01) as compared to cyclophosphamide in scoring of PCE: NCE ratio. All the groups were compared with cyclophosphamide (-ve control). So, it was concluded that the used polyherbal formulation (carwin) was found to be very effective drug in cancer therapy as it has very less genotoxic effect as compared to cyclophosphamide and also has a protective effect with cyclophosphamide. Keywords: Carwin capsules, Cyclophosphamide, Micronucleus assay, Antigenotoxic, Polyherbal formulation.

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“…The comet assay is a technique used for detecting the presence of single strand breaks of DNA at alkali-sensitive sites or sites of incomplete excision repair in mammalian cells (OECD, 2016). These tests have been used to analyze the toxicity (Araújo et al, 2015;Oliveira et al, 2016;Ramos et al, 2019) as well as anti-genotoxic, anti-mutagenic, and anti-carcinogenic effects (Lemes et al, 2017;Makhuvele et al, 2018) of extracts and isolated compounds from plants.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage

Oliveira

Freitas

Paiva

et al. 2020

Heliyon

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Pilosocereus gounellei (Cactaceae) is used to treat wounds and inflammation. In this study, we evaluated whether the saline extract from its stem would have genotoxic or anti-genotoxic effects. In the genotoxicity evaluation, mice received the extract (500, 1,000, or 2,000 mg/kg) orally while negative and positive controls were treated with saline solution (0.9% NaCl) per os and cyclophosphamide (CPA, 80 mg/kg i.p.), respectively. In the antigenotoxicity assay, using other animals, treatments were carried out by administering the extract (500, 1,000 or 2,000 mg/kg) or saline solution (negative control) per os and then CPA (80 mg/kg i.p.) 1 h later. Genotoxic effects were evaluated by micronucleus test and comet assay using peripheral blood and bone marrow cells. Oral administration of only the extract at 500 and 1,000 mg/kg did not result in genotoxicity. A slight increase in the incidence of micronucleus was observed at the highest dose (2,000 mg/kg). Administration of the extract before CPA reduced the micronucleated polychromatic erythrocytes (MNPCE) number by 49.07-71.43%, and DNA fragmentation in peripheral blood (85.04-94.44%) and bone marrow (87.43-92.70%) cells also decreased. In conclusion, when administered orally at the tested doses, the extract is genotoxically safe, being cautious in doses above 1,000 mg/kg, and has a protective effect against CPA-induced DNA damage.

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Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Cited by 10 publications

References 40 publications

Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

Chemoprotective Effect of Carwin Capsules against Cyclophosphamide Induced Genotoxicity by In Vivo Micronucleus Assay

Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage

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