Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts

Pinto, Nícolas de Castro Campos; Silva, Jucélia B; Menegati, Laura Machado; Guedes, Maria Clara Machado Resende; Marques, Lucas Bonfim; Silva, Thiago P Da; Melo, Rossana C. N.; Souza-Fagundes, Elaine M.; Salvador, Marcos José; Scio, Elita; Fabri, Rodrigo Luiz

doi:10.1590/0001-3765201720150702

Cited by 24 publications

(20 citation statements)

References 53 publications

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“…Indeed, infections caused by S. aureus have reached epidemic proportions globally owing to its strong multi-resistance [ 62 ]. These results were consistent with those of Pinto et al [ 58 ] who found the significant antibacterial activity of the methanolic extract of A. squamosa leaves and seeds against S. aureus , K. pneumoniae , and E. faecalis strains. Vijayalakshmi and Nithiya evaluated the antibacterial activity of A. squamosa fruit extracts, which exhibited more potent activity against gram-negative bacteria than gram-positive bacteria.…”

Section: Discussionsupporting

confidence: 93%

“…However, Zorofchian Moghadamtousi et al [57] demonstrated that the ethyl acetate extract of A. muricata leaves conspicuously blocked the migration of HCT-116 cells, suggesting the similar chemical composition of the leaves of both species (A. muricata and A. squamosa). A more recent study by Pinto [58] investigated the effects of A. squamosa seeds and leaves extracts on different tumor and non-tumor cells' ability to form colonies. The authors reported that both extracts ultimately reduced the colonogenic survival of MCF-7 and HCT-116 cells, while both extracts showed lower activity against non-tumor cells (VERO).…”

Section: Discussionmentioning

confidence: 99%

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GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

Al-Nemari

Al-Senaidy

Semlali

et al. 2020

BMC Complement Med Ther

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Background The research and application of plants in food supplements and drugs have attracted great interest. This study aimed to examine the efficiency of several solvents for the extraction of the main compounds from Annona squamosa leaves and to evaluate the antioxidant, antibacterial, and anticancer activities of these extracts. Methods Gas chromatography-mass spectrometry was used to screen the bioactive compounds of A. squamosa methanolic extract. The free radical, hydrogen peroxide, and nitric oxide scavenging activities of the extracts were investigated. Furthermore, MTT, nuclear staining, LDH, and monolayer wound repair assays were performed to evaluate the potential anticancer activity of the extracts in colon cancer cells while the antibacterial activity was tested by using a well diffusion assay. Results A. squamosa leaves extracts were found to contain several bioactive compounds, of which the majority were sesquiterpenes (C15H24). These extracts exhibited strong antioxidant activity and antibacterial potency against both gram-positive and gram-negative bacteria. Different A. squamosa leaves extracts displayed remarkable antiproliferative, cytotoxic, antimigration, and apoptotic activities in colon cancer cells. Conclusions A. squamosa leaves contain major bioactive compounds that inhibit the growth of several types of bacteria and colon cancer cell lines, which demonstrated their efficacy as an alternative source of antibiotics and for the development of novel drugs for colon cancer therapy.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

Al-Nemari

Al-Senaidy

Semlali

et al. 2020

BMC Complement Med Ther

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“…For this study, 10 10 CFU per ml of exponentially growing bacterial cultures were incubated with different concentrations of Cr‐SPs (0·5–8 mg ml −1 ) along with a control sample at 37°C in GM3 media. The growth was measured from 0 h till 48 h by recording the optical density at 595 nm at different time points and the growth curve is obtained by plotting absorbance against time (Pinto et al ). CFU assay was performed as mentioned in Zhou et al .…”

Section: Methodsmentioning

confidence: 99%

Evaluating the antibacterial and antibiofilm potential of sulphated polysaccharides extracted from green algaeChlamydomonas reinhardtii

Vishwakarma

Vavilala

2019

J Appl Microbiol

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Aim of the study In this study, antibacterial and antibiofilm potential of sulphated polysaccharides (SPs) extracted from Chlamydomonas reinhardtii (Cr) was evaluated against Neisseria mucosa, Escherichia coli, Streptococcus sp. and Bacillus subtilis. Methods and Results Antibacterial potential of Cr‐SPs was evaluated by agar‐cup diffusion, time‐kill and colony‐forming ability (CFU), minimum inhibitory and bactericidal concentration assays. Antibiofilm potential was evaluated by biofilm inhibition, eradication, extracellular‐DNA, metabolic activity and microscopy assays. Cr‐SPs at 0·5 mg ml−1 showed 34·52, 48·6, 66·1 and 55·6% reduced CFU in B. subtilis, Streptococcus, N. mucosa and E. coli respectively. Minimum inhibitory concentration of Cr‐SPs was as low as 480 μg ml−1 for Streptococcus, N. mucosa and 420 μg ml−1 for B. subtilis and E. coli. At 1 mg ml−1, Cr‐SPs showed 50% biofilm inhibition, whereas 4–8 mg ml−1 showed 100% inhibition. Cr‐SPs also effectively dissolved preformed biofilms. Dose‐dependent reduction in extracellular DNA revealed that Cr‐SPs interacts with the extra polymeric substance of the biofilm and destroys them. Light microscopy reconfirmed the above results. Conclusion Cr‐SPs not only inhibited biofilm formation but also effectively dissolved preformed‐biofilms. Significance and Impact of the Study The current study showed the promising potential of Cr‐SPs as antibiofilm agents. Further validation will help in developing Cr‐SPs as natural antibiotics against biofilm‐causing bacteria.

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“…Saline suspensions of freshly grown bacterial strains (10 8 CFU mL -1 ) were inoculated with different concentrations of psychorubrin (MIC, 0.5MIC, and 0.25MIC values) supplemented with MHB. Optical density (OD) at 600 nm was recorded in a spectrophotometer (Multiskan Go, Thermo Scientific, Waltham, MA, United States) at 2, 4, 6, 8, and 24 h of bacterial growth at 37°C as before ( Pinto et al, 2017a , b ). Graphs of turbidity versus incubation time were plotted.…”

Section: Methodsmentioning

confidence: 99%

“…Because antimicrobial resistance and biofilm formation are a global public health challenge, there is a striking need of development of new biologically active molecules against multidrug-resistant bacteria and the adoption of medicinal crude extracts of plants to treat infectious diseases ( Subramani et al, 2017 ). In this context, our group has been studying different potential bioactive antibacterial compounds ( Pinto et al, 2017a , b ), including a natural product termed psychorubrin, a napthoquinone commonly found in a variety of plants ( Fabri et al, 2009 , 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antibiofilm Activities of Psychorubrin, a Pyranonaphthoquinone Isolated From Mitracarpus frigidus (Rubiaceae)

et al. 2018

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Psychorubrin, a natural pyranonaphthoquinone found in different plants, has become an interesting compound in the search for new antimicrobial therapeutic agents. Here, we investigated the potential antagonistic activity of psychorubrin against planktonic and biofilm bacteria. First, psychorubrin was tested against several Gram-positive and Gram-negative bacteria strains by a broth microdilution susceptibility method. Second, bacterial killing assay, bacterial abundance, and membrane viability were evaluated. The nucleotide leakage assay was used to verify membrane destabilization while antibiofilm activities were analyzed by the effect on established biofilm, static biofilm formation, isolation of biofilm matrix assay and scanning electron microscopy. In parallel, the combinatorial effect of psychorubrin and chloramphenicol was evaluated by the checkerboard method. Psychorubrin was active against Gram-positive bacteria, showing rapid time-dependent kinetics of bacterial killing, amplified nucleotide leakage, and greater activity against the methicillin-resistant species (MRSA) Staphylococcus aureus 33591 and 33592 and Staphylococcus pyogenes 10096. Psychorubrin also interfered with the composition of the biofilm matrix by reducing the total content of carbohydrates and proteins. A synergic effect between psychorubrin and chloramphenicol was observed for S. aureus 33592 and S. pyogenes 10096 while an additive effect was detected for S. aureus 33591. Our findings demonstrate, for the first time, an antagonistic activity of psychorubrin against bacteria not only in their planktonic forms but also in biofilms, and identify bacterial membranes as primary targets for this compound. Based on these observations, psychorubrin has a good potential for the design of novel antimicrobial agents.

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Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts

Cited by 24 publications

References 53 publications

GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

Evaluating the antibacterial and antibiofilm potential of sulphated polysaccharides extracted from green algaeChlamydomonas reinhardtii

Antibacterial and Antibiofilm Activities of Psychorubrin, a Pyranonaphthoquinone Isolated From Mitracarpus frigidus (Rubiaceae)

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