Post-embriononic development of Chrysomya putoria(Diptera: Calliphoridae) on a diet containing ampicillin in different concentrations

Ferraz, Adriana Cristina Pedroso; Dallavecchia, Daniele Lourinho; Silva, Débora C.; Silva-Filho, Renato G.; Aguiar, Valéria Magalhães

doi:10.1590/0001-3765201620140440

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…On this subject, Ferraz et al . (, b; ) tested the development of C . putoria using a diet‐containing antibiotics (ampicillin, gentamicin and ciprofloxacin) in different concentrations, to study the species' growth rate under the effect of these substances.…”

Section: Introductionmentioning

confidence: 99%

Post‐embryonic development of Chrysomya putoria (Diptera: Calliphoridae) in swine and bovine tissues in South America: implications for forensic entomology

2017

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Necrophagous diptera are used in forensic entomology to measure the post-mortem interval (PMI) of cadavers. PMI estimates based on fly larvae, however, can be inaccurate when larval development is retarded, or accelerated, by the nutritional composition of the cadaveric tissue. We compared the post-embryonic development of Chrysomya putoria (Wiedemann, 1830) on different tissues of pigs and cows. Forty newly hatched fly larvae per treatment were placed on 60 g of the following swine tissues: liver (T1), swine lung (T2), swine heart (T3), swine muscle (T4), bovine heart (T5), bovine lung (T6) and bovine muscle (T7). There were four replicates/treatment. Experiments were conducted in controlled conditions (28°C day/26°C night, RH 70% ± 10% and 12 h of photophase). Fly larvae reared on swine liver took longer to develop (5.2 days) than flies reared on other substrates (from 3.5 to 4.5 days), and this difference was statistically significant. The total duration of development on swine liver (T1) was significantly slower (8.7 days) when compared to the other treatments. Larvae reared on swine lungs (T2) were significantly heavier in the third instar (x = 61.2 mg) than larvae reared on swine liver (T1), swine muscle (T4), bovine lung (T6) and bovine muscle (T7). Total larval survival was high (above 76%) in all treatments except for T1 (66%). There was no deviation from the expected adult sex ratio, and the percentage of abnormalities remained within the expected range. These results reinforce the idea that, to achieve greater accuracy in PMI estimates, the type of tissue on which the larvae develops needs to be taken into account.

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Section: Introductionmentioning

confidence: 99%

Post‐embryonic development of Chrysomya putoria (Diptera: Calliphoridae) in swine and bovine tissues in South America: implications for forensic entomology

2017

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The implication of morphometrics and growth rate of dipteran flies in forensic entomotoxicology research: a review

Bhardwaj

Sharma

Dalal

et al. 2020

Sci Nat

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Chronology of the Intrapuparial Development of the Blowfly Chrysomya albiceps (Diptera: Calliphoridae): Application in Forensic Entomology

Salazar-Souza

Couri

Aguiar

2018

Journal of Medical Entomology

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Insects display different patterns of development, and blow flies have one of the most specialized patterns of intrapuparial development of all. In forensic entomology, pupae can be used as a tool to estimate the minimum postmortem time interval (minPMI). We analyzed the intrapuparial development of Chrysomya albiceps (Diptera: Caloricidade), whose larvae had been fed pig lungs and reared in a climate-controlled room at 28°C day/26°C night, 70 ± 10% RH, and 12 h of photophase and monitored daily. After the third-instar larvae abandoned their diet, the process of pupariation and pupation was monitored. At pre-established times, five pupae were collected, euthanized, and fixed in 5% formaldehyde, inside polypropylene test tubes with caps. Since they were the first, they were classified as 0 h pupae. Twelve collections occurred until the emergence of the adults, at 0, 2, 4, 6, 8, 10, 24, 30, 48, 54, 72, 78, 96, and 99 h (n = 84). The fixed pupae were dissected under the microscope, with the aid of anatomical tweezers and hypodermic needles, and photographed. The stages of metamorphosis and the morphological alterations occurring during the process were identified, described, and recorded before and after pupation. These phases were: pupation, larval pupal apolysis, cryptocephalic, phanerocephalic, pharate adult, emergence, and adult. The cryptophalic phase occurred between 4 and 6 h after pupation; the phanerocephalic phase between 6 and 10 h after; the pharate adult phase between 24 and 96 h after; and the imago/emergence phase 99 h after pupation.

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Post-embriononic development of Chrysomya putoria(Diptera: Calliphoridae) on a diet containing ampicillin in different concentrations

Cited by 5 publications

References 32 publications

Post‐embryonic development of Chrysomya putoria (Diptera: Calliphoridae) in swine and bovine tissues in South America: implications for forensic entomology

Post‐embryonic development of Chrysomya putoria (Diptera: Calliphoridae) in swine and bovine tissues in South America: implications for forensic entomology

The implication of morphometrics and growth rate of dipteran flies in forensic entomotoxicology research: a review

Chronology of the Intrapuparial Development of the Blowfly Chrysomya albiceps (Diptera: Calliphoridae): Application in Forensic Entomology

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