Fermentation characteristics in stirred-tank reactor of exopolysaccharides with hypolipidemic activity produced by Pleurotus geesteranus 5#

Abstract

In this study, the hypolipidemic effect of exopolysaccharides (EPS) from Pleurotus geesteranus 5 # fermenting liquor by the optimal culture conditions in a 5-L stirred-tank reactor was investigated. The hypolipidemic effect of the polysaccharide, investigated in streptozotocin induced diabetic mice, decreased plasma glucose, total cholesterol and triacylglycerol concentrations by 17.1 %, 18.8 % and 12.0 %, respectively. The results of the present investigation strongly demonstrate the potential of this polysaccharide to prevent hyperglycemia in the experimental animals. Under optimal culture conditions, the maximum concentrations of mycelial and EPS were 22.63 g/L after 7 d cultivation and 11.09 g/L after 10 d, respectively. Furthermore, the morphological parameters (i.e. mean diameter, circularity, roughness and compactness) of the pellets and the broth viscosity were characterized. It was proved that compactness of the pellet morphology (R 2 =0.963, p<0.01) was significantly and positively determined with mycelial biomass. Moreover, mean diameter (R 2 =93.3, p<0.01) and broth viscosity (R 2 =0.950, p<0.01) were significantly and positively determined with EPS content.

Characterization of the Metabolic Response of Streptomyces clavuligerus to Shear Stress in Stirred Tanks and Single-Use 2D Rocking Motion Bioreactors for Clavulanic Acid Production

“…The shear stress values (τ max ) in the STR reactor presented in Table 1 were consistent with results from previous studies on cultivation of filamentous organisms in stirred tank bioreactors [3,31]. Shear stress higher than 6 Pa, as observed in STR reactors, triggers stress responses in sensitive strains like CHO cells.…”

Mushroom Polysaccharides: Chemistry and Antiobesity, Antidiabetes, Anticancer, and Antibiotic Properties in Cells, Rodents, and Humans

“…The polysaccharides seem to act as PPAR-α agonists that result in decreased serum triglyceride and increased HDL levels by increasing lipid uptake, activation, and catabolism via transcriptional changes of genes that control these events. Duobin, et al [ 170 ] observed similar hypolipidemic properties of exopolysaccharides produced by Pleurotus geesteranus fermented mushrooms in diabetic mice.…”

Anti-inflammatory and hepatoprotective effects of exopolysaccharides isolated from Pleurotus geesteranus on alcohol-induced liver injury

“…As the most abundant metabolites of mushrooms, polysaccharides have gained much attentions owing to various biological activities including hepatoprotective 21 , anti-oxidative 25 , anti-inflammatory 26 , antitumour 27 , antiproliferative 28 and antihyperlipidaemic effects 29 , etc . Based on previous studies by academic researchers, EPS from P. geesteranus showed hypolipidaemic, antioxidative and antitumour activities 22 , 30 . However, the data regarding the chemical structure, hepatoprotective and anti-inflammatory effects of the EPS from P. geesteranus are limited.…”

“…In recent years, mushroom-derived natural products have been used as functional foods and represent the basis for the development of new biopharmaceuticals 21 . Pleurotus geesteranus , which belongs to phylum Basidiomycota , is rich in biologically active components, including polysaccharides, peptides, sterols and dietary fibre 22 – 24 . As the most abundant metabolites of mushrooms, polysaccharides have gained much attentions owing to various biological activities including hepatoprotective 21 , anti-oxidative 25 , anti-inflammatory 26 , antitumour 27 , antiproliferative 28 and antihyperlipidaemic effects 29 , etc .…”