Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)

Cardoso, Jean Carlos; Inthurn, Ana Carolina Petit

doi:10.14295/oh.v24i3.1222

Cited by 12 publications

(12 citation statements)

References 5 publications

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“…The most commonly used concentration is 0.003% of active chlorine. Higher concentrations may be phytotoxic to the cultures, as shown in in vitro cultures of Eucalyptus benthamii and Prunus mume [42]. In Eucalyptus benthamii, active chlorine concentrations above 0.005% reduced the percentage of viable shoots [16]; and in Prunus mume, the addition of 0.001% of active chlorine reduced explant survival percentage [43].…”

Section: Survival Of Explantsmentioning

confidence: 99%

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Atala

Faria

Molinari

et al. 2021

Preprint

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Micropropagation is one of the main applications of tissue culture of plants. It allows rejuvenation and, consequently, improves explant rooting of woody species. However, one of its main problems is the risk of microbial manifestation. Here, we aimed to evaluate the survival and the reduction in microbial manifestations on in vitro cultures of Eucalyptus microcorys with the addition of chemical agents (sodium hypochlorite or streptomycin) to WPM culture medium. We used in vitro cultivated explants from adventitious shoots from two E. microcorys mother plants, over at 44 years-old. We drew from the multiplication phase explants with clumps of 8 to 12 shoots that displayed bacterial manifestation and submitted them to microbe control, adding different chemical agents at different concentrations to the culture medium: sodium hypochlorite (NaClO) and the antibiotic streptomycin. The results indicated that the best concentrations to reduce the manifestation of microorganisms on in vitro cultivation were 100 mg L− 1 of streptomycin and sodium hypochlorite with 0.003% of active chlorine, since these concentrations maintained 100% of the explants alive and reduced microbial manifestation more efficiently.

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Section: Survival Of Explantsmentioning

confidence: 99%

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Atala

Faria

Molinari

et al. 2021

Preprint

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“…As low as 12.6 μM aqueous CD was capable of sterilizing potato culture media. This concentration was lower than the concentration of sodium hypochlorite (NaOCl) used to sterilize media in previous studies 17,18,31 , but similar to the CD concentrations used in other plant species 13–16 . Another advantage of using CD for sterilization is the low cost; the prices of sodium chlorite and food grade hydrochloric acid are far lower than commercial sodium hypochlorite products 11 .…”

Section: Discussionmentioning

confidence: 44%

“…In 2009, Cardoso successfully used CD to sterilize plant media to culture anthurium 13 . Afterwards, CD was applied to sterilize culture media for gerbera, and had similar sterilization efficiency as autoclaving, and resulted in similar or better growth of plants 14–16 . Our research group has evaluated the effectiveness of CD to disinfect explants to culture various plant species.…”

Section: Discussionmentioning

confidence: 99%

“…Chemical sterilization is advantageous in that it is more cost effective than autoclaving 11 . Accordingly, a number of chemicals, including diethyl pyrocarbonate 12 , chlorine dioxide 13–16 , peracetic acid 13 , sodium hypochlorite 17–19 , and hydrogen peroxide 19 , have been used to sterilize plant medium culture. However, chemical sterilization of media has not been tested in potato tissue culture, despite the need for improved conditions to reduce the cost of producing microtubers.…”

Section: Introductionmentioning

confidence: 99%

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Use of chlorine dioxide to sterilize medium for tissue culture of potato

Duan

Zhang

Sun

et al. 2019

Sci Rep

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In vitro cultured seedlings or microtubers are the major starting materials for the production of potato. Currently, seedlings are cultured in media sterilized by autoclaving, which, however, consumes more electricity and takes longer for sterilization, and also requires high temperature-tolerant vessel materials. In order to identify alternative methods of sterilizing culture conditions, the disinfection effects of chlorine dioxide (CD) at 88.0, 29.3, 17.6, 12.6 and 8.8 μM were evaluated in potato medium and vessels. The ≥12.6 μM gaseous CD effectively disinfected vessel through a 30-min fumigation process, and its aqueous solution disinfected potato medium efficiently as well. In presence of 12.6 μM CD in the medium, the potato seedlings had similar morphological features as those grown on autoclaved medium, with some exceptions. The use of 12.6–29.3 μM aqueous CD to sterilize the medium increased antioxidant enzyme activities in potato seedlings, while the use of higher concentration decreased antioxidant enzyme activity levels. SSR analysis did not reveal significant molecular differences in potato seedlings cultured between autoclaved and CD-sterilized medium. In addition to this, CD-sterilized medium induced potato microtuber formation at a similar rate as autoclaved medium. In summary, using CD to sterilize potato medium and vessels did not compromise the growth of seedlings and microtuber induction. This study provides an economical and simplified sterilization method for media used to culture potato plantlets, and this can improve energy use of the large-scale tissue culture industry.

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“…The Gerbera (G. jamesonii L. ) is one of the most important ornamental species for the cut flowers sector (Cardoso & Imthurn, 2018), and is traditionally known for its variety of colors and shapes (Bashandy et al, 2015). The flower is among the most commercialized cut flowers in the national market and its beauty causes consumer acceptance (Menegaes et al, 2015;Sulzbach et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Crop coefficient of cut gerbera with water supplementation in a protected environment

Piroli

Peiter

Robaina

et al. 2020

Agraria

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The determination of the crop coefficient (Kc) is vital since, although gerbera cultivation is constantly expanding, there is little research on the evapotranspiration of the crop cultivated under a protected environment to obtain the correct irrigation management. The objective of this work was to evaluate the evapotranspiration and crop coefficients of cut gerbera, cultivated in protected environment. The reference evapotranspiration (ETo) and crop evapotranspiration (ETc) were obtained through the Penman and weighing lysimetry methods, respectively. The experimental design used was completely randomized, consisting of five irrigation treatments (120, 100, 80, 60, and 40% of the water retention capacity), with ten replicates each, totalizing 50 experimental units. The Kc was evaluated using the irrigation lamina that presented the highest production of floral stems among the tested treatments, obtained through the relation between ETc and ETo. The mean of the crop coefficient ranged from 0.72 in vegetative period I, 0.81 in vegetative period II, 0.85 in flowering I, 0.89 in full flowering II, 1.33 in full flowering III, and 1.06 in end of the flowering I. The water consumption for the cut gerbera crop in the treatments with water availability ranged from 225.87 to 523.58 mm. Therefore, the crop coefficients proposed for the different phenological stages of the gerbera were efficient for irrigation management.

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Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)

Cited by 12 publications

References 5 publications

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Use of chlorine dioxide to sterilize medium for tissue culture of potato

Crop coefficient of cut gerbera with water supplementation in a protected environment

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