Advances in biotechnology and genomics of switchgrass

Nageswara-Rao, Madhugiri; Soneji, Jaya R.; Kwit, Charles; Stewart, C. Neal

doi:10.1186/1754-6834-6-77

Cited by 41 publications

(32 citation statements)

References 130 publications

(208 reference statements)

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“…The application of biotechnology for switchgrass improvement has been reviewed recently [17]. There is also a large body of literature on many of the downstream aspects for the utilization of switchgrass biomass for conversion to fuels using biochemical or thermochemical platforms.…”

Section: Switchgrass As a Temperate C 4 Warm-season Grass Modelmentioning

confidence: 99%

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Senescence, dormancy and tillering in perennial C4 grasses

2014

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a b s t r a c tPerennial, temperate, C 4 grasses, such as switchgrass and miscanthus have been tabbed as sources of herbaceous biomass for the production of green fuels and chemicals based on a number of positive agronomic traits. Although there is important literature on the management of these species for biomass production on marginal lands, numerous aspects of their biology are as yet unexplored at the molecular level. Perenniality, a key agronomic trait, is a function of plant dormancy and winter survival of the belowground parts of the plants. These include the crowns, rhizomes and meristems that will produce tillers. Maintaining meristem viability is critical for the continued survival of the plants. Plant tillers emerge from the dormant crown and rhizome meristems at the start of the growing period in the spring, progress through a phase of vegetative growth, followed by flowering and eventually undergo senescence. There is nutrient mobilization from the aerial portions of the plant to the crowns and rhizomes during tiller senescence. Signals arising from the shoots and from the environment can be expected to be integrated as the plants enter into dormancy. Plant senescence and dormancy have been well studied in several dicot species and offer a potential framework to understand these processes in temperate C 4 perennial grasses. The availability of latitudinally adapted populations for switchgrass presents an opportunity to dissect molecular mechanisms that can impact senescence, dormancy and winter survival. Given the large increase in genomic and other resources for switchgrass, it is anticipated that projected molecular studies with switchgrass will have a broader impact on related species.

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Section: Switchgrass As a Temperate C 4 Warm-season Grass Modelmentioning

confidence: 99%

“…Specific roles of key genes will need to be validated by transgenic routes. These studies can be performed in switchgrass [17,18], but they can be relatively time consuming. Potentially other model systems such as Brachypodium, Setaria italica and Panicum halli could be used which are easier to transform and analyze [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Senescence, dormancy and tillering in perennial C4 grasses

2014

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“…So far no genetic transformation of upland cultivars has been reported. Even callus regeneration is very difficult to achieve (Song et al 2012;Nageswara-Rao et al 2013). Thus, establishment of a highly efficient and amenable plant regeneration and transformation system of switchgrass is still in urgent need.…”

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confidence: 99%

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

et al. 2015

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Selection of pre-embryogenic callus from a core structure from mature seed-derived callus is the key for high-efficiency plant regeneration and transformation of switchgrass different cultivars. Switchgrass (Panicum virgatum L.) has been identified as a dedicated biofuel crop. For its trait improvement through biotechnological approaches, we have developed a highly efficient plant regeneration and genetic transformation protocol for both lowland and upland cultivars. We identified and separated a pre-embryogenic "core" structure from the seed-derived callus, which often leads to development of highly regenerative type II calluses. From the type II callus, plant regeneration rate of lowland cultivars Alamo and Performer reaches 95%, and upland cultivars Blackwell and Dacotah, 50 and 76%, respectively. The type II callus was also amenable for Agrobacterium-mediated transformation. Transformation efficiency of 72.8% was achieved for lowland cultivar Alamo, and 8.0% for upland cultivar Dacotah. PCR, Southern blot and GUS staining assays were performed to verify the transgenic events. High regenerative callus lines could be established in 3 months, and transgenic plants could be obtained in 2 months after Agrobacterium infection. To our knowledge, this is the first report on successful plant regeneration and recovery of transgenic plants from upland switchgrass cultivars by Agrobacterium-mediated transformation. The method presented here could be helpful in breaking through the bottleneck of regeneration and transformation of lowland and upland switchgrass cultivars and probably other recalcitrant grass crops.

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“…In switchgrass, a great number of investigations have focused on improving bioethanol relevant traits by the aforementioned approaches of classical breeding or genomic technologies [209,210]. Notably, crosses between tetraploid upland and lowland switchgrass resulted in hybrid vigor and progeny displayed increase of biomass yields in the range of 30-60% pointing to exploiting heterosis for generating elite cultivars [211].…”

Section: Cinnamyl Alcohol Dehydrogenase (Cad) Caffeic Acid 3-o-methymentioning

confidence: 99%

Biofuels Get in the Fast Lane: Developments in Plant Feedstock Production and Processing

Triantafyllidis¹

2013

Adv Crop Sci Tech

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In recent years, high volatility in oil prices and global climate change led to an increased interest in biofuel production to reduce dependency on foreign fossil fuel. Domestically produced plant feedstocks are environmentally friendly renewable substitutes for fossil-derived fuel and are expected to stabilize fuel prices. Plant-derived energy can offer rural development and other environmental, social and energy security benefits for local societies. Crops, grasses, trees, forest-residues and aquatic plants, all can be used as potential biofuel feedstocks. To meet the increased global and regional demand for bioenergy, evaluation and improvement of current and emergent plant feedstocks is urgently needed to reduce the cost of the resulting biofuels.

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Advances in biotechnology and genomics of switchgrass

Cited by 41 publications

References 130 publications

Senescence, dormancy and tillering in perennial C4 grasses

Senescence, dormancy and tillering in perennial C4 grasses

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

Biofuels Get in the Fast Lane: Developments in Plant Feedstock Production and Processing

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