Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon

Abstract

BackgroundThe Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date. In this study, the results of an indirect sandwich IgG ELISA, standardized for 19 arbovirustypes circulating among horses in Brazilian Amazon, were compared to results of the hemagglutination inhibition test. A screening test assessed the conditional probability distribution and a Pearson linear correlation test determined the correlation strength among the absorbance values recorded for viruses from the same family.FindingsSensitivity varied between 40.85 and 100%; the specificity was low and ranged from 39.71 to 67.0%; and the accuracy varied between 41 and 65.2%. The test developed in this study yielded a large number of serological cross-reactions.ConclusionsThe test can be employed to detect IgG antibodies within one arbovirus family; however, the hemagglutination test or other more specific techniques, such as the serum neutralization test in mice or the plaque-reduction neutralization test, are essential complementary methods for positive cases.

O risco na pesquisa de campo em saúde na Amazônia brasileira: mais de um século de desafios (1901-2015)

“…As evidências de circulação de arbovírus de importância médica, entre animais domésticos e de produção rural, como cavalos e búfalos, repercutem tanto para a suscetibilidade da população da região, como também para os pesquisadores que coletam e manipulam essas amostras 36,40,57 .…”