Tick-borne blood cell pathogens, which are challenging to diagnose, are primarily detected using molecular techniques. Therefore, this study aimed to detect the main infectious agents involved in 50 cases of suspected hemoparasitosis in dogs treated at the Veterinary Hospital Mário Dias Teixeira of the Federal Rural University of the Amazon. Hematological parameters were evaluated, and blood samples were subjected to polymerase chain reaction (PCR) assays for DNA amplification of the following species: Ehrlichia canis, Anaplasma platys, and Babesia canis. The PCR test results indicated that the most prevalent infectious agent was E. canis, present in 12% (6/50) infected animals, followed by A. platys and B. canis, present in 8% (4/50) and 2% (1/50) infected animals, respectively. Regarding hematological analysis, the most relevant changes were anemia, lymphopenia, thrombocytopenia, leukocytosis, and leukopenia. The availability of molecular techniques allows the management of the most appropriate treatment to infected animals in a rapid and specific way.

Many human arboviruses are also pathogenic for horses, and some of these have emerged recently. A descriptive cross-sectional observational study was conducted to assess the prevalence of West Nile virus (WNV) and other arboviruses among 77 horses on the rural properties of the Espirito Santo state, Brazil. Serum samples were screened for arbovirus-reactive antibodies using the hemagglutination inhibition technique and subsequently a plaque reduction neutralization test for the confirmation of exposure from sera was used to detect heterotypic immune reactions. Overall, the total antibodies against at least one arbovirus of Alphavirus, Flavivirus, and Orthobunyavirus genera were detected in 39 (50.6%) animals. The antibodies to Phlebovirus were not detected in any sample. When the 24 WNV hemagglutination inhibition (HI)-positive samples were tested by the plaque-reduction neutralization test 90%, 9 (32.1%) were positive for WNV antibodies and 14 (50%) for Saint Louis encephalitis virus. Our findings indicate that the region provides ideal conditions for the emergence of arboviruses, reinforcing the need for further surveillance of mosquito-transmitted diseases in domestic animals.

Feline sporotrichosis is a subcutaneous, infectious, and contagious mycosis, with zoonotic characteristics. Until now there are no reports on the isolation of Sporothrix sp. in cats in the state of Pará. The present work aimed to isolate Sporothrix spp. from a cat with possible zoonotic involvement in the city of Belém, Pará. Through the active search for suspected cases of sporotrichosis, carried out by members of the Center for the Control of Zoonoses of Belém (CCZ, Belém) the suspicion of the disease in a feline was reported, with probable transmission to its owner. Clinical evaluation was performed, and biological material was collected from the animal for identifying the agent. Microscopic evaluation of the collected sample and microbial culture confirmed the presence of Sporothrix sp. in the sample. This study is the first to report the microbiological isolation of Sporothrix sp. in a cat in the metropolitan region of Belém, Pará, with possible zoonotic transmission. This indicates a probable underreporting of cases, an unknowing regarding the reality of health conditions and the need for more accurate and comprehensive evaluation of sporotrichosis in this region.