Emerging roles of microRNAs in the mediation of drought stress response in plants

Ding, Yanfei; Ya, Tao; C, Zhu

doi:10.1093/jxb/ert164

Cited by 246 publications

(168 citation statements)

References 78 publications

Supporting

Mentioning

163

Contrasting

Order By: Relevance

“…The TIR1 enzyme is a positive regulator of auxin signalling by promoting the degradation of Aux/IAA proteins through ubiquitination. Thus, increased levels of miR393 would down regulate auxin signalling and may reduce plant growth under drought stress (Ding et al, 2013). It was also shown in this study that miR393 was down regulated more than two folds in KC3-WS; Supplementary Table 1) which might favour the growth of KC3 under drought.…”

Section: Targets Of Mirnas Identified In This Studysupporting

confidence: 54%

“…Overexpression of miR396 also conferred increased tolerance to drought stress in tobacco (Yang and Yu, 2009). These observations suggest that miR396 plays important roles not only in leaf development but also in drought tolerance in plants (Ding et al, 2013).…”

Section: Targets Of Mirnas Identified In This Studymentioning

confidence: 85%

“…It is known that miR393 was commonly up regulated during drought stress in Arabidopsis, rice and sugarcane (Ding et al, 2013). The target of miR393 encodes TIR1 (transport inhibitor response 1), an auxin receptor in Arabidopsis.…”

Section: Targets Of Mirnas Identified In This Studymentioning

confidence: 99%

See 2 more Smart Citations

Comparative miRNAome Analysis Revealed Numerous Conserved and Novel Drought Responsive miRNAs in Cotton (<i>Gossypium spp.</i>)

Boopathi

Sathish²,

Kavitha³

et al. 2016

cgg

View full text Add to dashboard Cite

Negative regulations of gene expression by endogenous, non-coding miRNAs have been shown to play important role in abiotic stress responses in plants. However, limited knowledge is available on water stress responsive miRNAs in cotton. In this study, differentially expressed drought responsive miRNAs and their targets were identified under natural field conditions th rough high-throughput small RNA sequencing by comparing leaf samples of drought tolerant Gossypium hirsutum cv. KC3 and drought susceptible G. barbadense cv. Suvin. Totally four small RNA libraries were constructed and sequenced by employing ion proton TM technology. Altogether, there were 39 407 089 reads with a mean read length of 22 nt. In KC3, there were 5 138 unique miRNA reads that were differentially expressed with at least two folds under water stressed conditions. In contrast, Suvin have sho wn 8 469 unique miRNA reads that were differentially expressed with minimum of two folds under water stressed conditions. Comparison of miRNAs expressed under water stressed conditions between KC3 and Suvin, have resulted 7 494 miRNA reads and interestingly majority of them were down regulated with at least two folds. Besides identify ing large numbers of novel miRNAs, several abiotic stress responsive conserved miRNAs were also noticed. Of particular interest were miR750, miR2, miR14, miR276, miR279, miRbantam and miR5176 that were highly down regulated in KC3 under water stress conditions. Strikingly, miR2 and miR-bantam were previously shown to target pre-apoptotic genes in biological systems. Further, the identified miRNAs were also targeting different classes of dehydrogenases, protein kinases and transcription factors. Our results revealed for the first time that there were large numbers of water stress related miRNAs that might be sequentially and/or complexly involved in gene regulation that confers drought tolerance in cotton under field conditions and they have enormous potential in elucidating the molecular mechanism of miRNA based gene regulation and more importantly in genetic improvement of drought tolerance in cotton. Further, this is the first report on experimentally identifying miRNAs in G. barbadense.

show abstract

Section: Targets Of Mirnas Identified In This Studysupporting

confidence: 54%

Section: Targets Of Mirnas Identified In This Studymentioning

confidence: 85%

See 1 more Smart Citation

Comparative miRNAome Analysis Revealed Numerous Conserved and Novel Drought Responsive miRNAs in Cotton (<i>Gossypium spp.</i>)

Boopathi

Sathish²,

Kavitha³

et al. 2016

cgg

View full text Add to dashboard Cite

show abstract

“…The comparative analysis of sRNA profiles confirms that the extensive differences in gene expression between the Dactylorhiza species are complemented by divergent post‐transcriptional regulations. miRNAs function as negative regulators of gene expression via translational repression or mRNA cleavage (Borges & Martienssen, 2015) and are known to mediate response to genomic or environmental stresses (Ding, Tao, & Zhu, 2013; Ivashuta et al., 2011). From the transcripts that were targeted by miRNAs and/or tasiRNAs, 27.2% were found to be differentially regulated between the two orchid species, with deviating post‐transcriptional regulation of metabolism (e.g., nucleic acids, aromatic compounds, tetrapyrrole and alkaloid metabolism targeted in D. fuchsii , and DNA and tetrapyrrole metabolism targeted in D. incarnata ) and binding (Fig.…”

Section: Discussionmentioning

confidence: 99%

Adaptive sequence evolution is driven by biotic stress in a pair of orchid species (Dactylorhiza) with distinct ecological optima

et al. 2017

View full text Add to dashboard Cite

The orchid family is the largest in the angiosperms, but little is known about the molecular basis of the significant variation they exhibit. We investigate here the transcriptomic divergence between two European terrestrial orchids, Dactylorhiza incarnata and Dactylorhiza fuchsii, and integrate these results in the context of their distinct ecologies that we also document. Clear signals of lineage‐specific adaptive evolution of protein‐coding sequences are identified, notably targeting elements of biotic defence, including both physical and chemical adaptations in the context of divergent pools of pathogens and herbivores. In turn, a substantial regulatory divergence between the two species appears linked to adaptation/acclimation to abiotic conditions. Several of the pathways affected by differential expression are also targeted by deviating post‐transcriptional regulation via sRNAs. Finally, D. incarnata appears to suffer from insufficient sRNA control over the activity of RNA‐dependent DNA polymerase, resulting in increased activity of class I transposable elements and, over time, in larger genome size than that of D. fuchsii. The extensive molecular divergence between the two species suggests significant genomic and transcriptomic shock in their hybrids and offers insights into the difficulty of coexistence at the homoploid level. Altogether, biological response to selection, accumulated during the history of these orchids, appears governed by their microenvironmental context, in which biotic and abiotic pressures act synergistically to shape transcriptome structure, expression and regulation.

show abstract

“…However, the yield of alfalfa is often constrained by diverse abiotic stresses including drought. Drought is a common environmental stresses, affecting plants' productivity [4,5]. Thus understanding the molecular mechanism of alfalfa responding to drought stress becomes a necessary step in improving the drought tolerance of alfalfa.…”

Section: Introductionmentioning

confidence: 99%

Identification of Drought-Responsive microRNAs from Roots and Leaves of Alfalfa by High-Throughput Sequencing

Li¹,

Wan²,

Bi³

et al. 2017

Preprint

View full text Add to dashboard Cite

Alfalfa, an important legume forage, is an ideal crop for sustainable agriculture and a potential bioenergy plant. Drought, one of the most common environmental stresses, substantially affects plants' growth, development and productivity. MicroRNAs (miRNAs) are newly discovered gene expression regulators that have been linked to several plant stress responses. To elucidate the role of miRNAs in drought stress regulation of alfalfa, a high-throughput sequencing approach was used to analyze 12 small RNA libraries comprising of 4 samples, each with 3 biological replicates. We identified 348 known miRNAs, belonging to 80 miRNA families, from the 12 libraries and 281 novel miRNAs using Mireap software. 18 known miRNAs in roots and 12 known miRNAs in leaves were screened out as drought-responsive miRNAs. Except for miR319d and miR157a which were upregulated under drought stress, the expression pattern of drought-responsive miRNAs were different between roots and leaves in alfalfa. This is the first study discovering miR157a, miR1507, miR3512, miR3630, miR5213, miR5294, miR5368 and miR6173 are drought-responsive miRNAs. Target transcripts of drought-responsive miRNAs were computationally predicted. All 447 target genes for the known miRNAs were predicted using an online tool. This study provides a significant insight on understanding drought-responsive mechanisms of alfalfa.

show abstract

Emerging roles of microRNAs in the mediation of drought stress response in plants

Cited by 246 publications

References 78 publications

Comparative miRNAome Analysis Revealed Numerous Conserved and Novel Drought Responsive miRNAs in Cotton (<i>Gossypium spp.</i>)

Comparative miRNAome Analysis Revealed Numerous Conserved and Novel Drought Responsive miRNAs in Cotton (<i>Gossypium spp.</i>)

Adaptive sequence evolution is driven by biotic stress in a pair of orchid species (Dactylorhiza) with distinct ecological optima

Identification of Drought-Responsive microRNAs from Roots and Leaves of Alfalfa by High-Throughput Sequencing

Contact Info

Product

Resources

About