Phylogeny and gene expression of the complete NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY in Triticum aestivum

Wang, Huadun; Wan, Yongfang; Büchner, Peter; King, Robert C.; Ma, Hongxiang; Hawkesford, Malcolm J.

doi:10.1093/jxb/eraa210

Cited by 38 publications

(35 citation statements)

References 78 publications

(118 reference statements)

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“…Unlike the high-affinity NRT2-NAR2 nitrate uptake system, the low-affinity NPF system consists of a large gene family in plants (e.g., Arabidopsis, 53 genes; rice, 93 genes) [ 6 ]. In wheat, 113 NPF genes (one from each homeologous group) have recently been identified [ 26 ]. This study investigated the mRNA levels of representative TaNPF genes ( Figure 6 A).…”

Section: Resultsmentioning

confidence: 99%

Physiological and Molecular Traits Associated with Nitrogen Uptake under Limited Nitrogen in Soft Red Winter Wheat

Lamichhane

Murata

Griffey

et al. 2021

Plants

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A sufficient nitrogen (N) supply is pivotal for high grain yield and desired grain protein content in wheat (Triticum aestivum L.). Elucidation of physiological and molecular mechanisms underlying nitrogen use efficiency (NUE) will enhance our ability to develop new N-saving varieties in wheat. In this study, we analyzed two soft red winter wheat genotypes, VA08MAS-369 and VA07W-415, with contrasting NUE under limited N. Our previous study demonstrated that higher NUE in VA08MAS-369 resulted from accelerated senescence and N remobilization in flag leaves at low N. The present study revealed that VA08MAS-369 also exhibited higher nitrogen uptake efficiency (NUpE) than VA07W-415 under limited N. VA08MAS-369 consistently maintained root growth parameters such as maximum root depth, total root diameter, total root surface area, and total root volume under N limitation, relative to VA07W-415. Our time-course N content analysis indicated that VA08MAS-369 absorbed N more abundantly than VA07W-415 after the anthesis stage at low N. More efficient N uptake in VA08MAS-369 was associated with the increased expression of genes encoding a two-component high-affinity nitrate transport system, including four NRT2s and three NAR2s, in roots at low N. Altogether, these results demonstrate that VA08MAS-369 can absorb N efficiently even under limited N due to maintained root development and increased function of N uptake. The ability of VA08MAS-369 in N remobilization and uptake suggests that this genotype could be a valuable genetic material for the improvement of NUE in soft red winter wheat.

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Section: Resultsmentioning

confidence: 99%

Physiological and Molecular Traits Associated with Nitrogen Uptake under Limited Nitrogen in Soft Red Winter Wheat

Lamichhane

Murata

Griffey

et al. 2021

Plants

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“…Nitrate and phytohormone signaling pathways crosstalk to modulate growth and developmental programs in a multifactorial manner [58]. So far, more than half of functionally characterized NPF genes have been demonstrated to be able to transport nitrate in Arabidopsis [13]. Here, twenty BnaNPF genes, ortholog to 11 AtNPF genes, were detected to respond to low nitrate treatment (Fig.…”

Section: Discussionmentioning

confidence: 83%

“…This study provides a piece of basic information for further functional characterization of BnaNPF genes in the growth and development of B. napus. Recently, in apple and wheat [13,39], the NPF protein family was characterized and also de ned as eight subfamilies, and according to eight subfamilies of NPF de ned in these species and phylogenetic analysis, the 169 BnaNPF genes were identi ed and classi ed into eight unambiguous subfamilies, and BnaNPF subfamilies showed similar member proportions with these in Arabidopsis and wheat [1,13]. B.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, some of NPF members are even able to transport more than one different substrate: nitrate/IAA, nitrate/ABA, nitrate/glucosinolates, peptides/amino acids, or GA/JA.After identifying the rst nitrate transporter NPF6.3/NRT1.1 [4], Tsay et al (1993) subsequently characterized more than half of the NPF nitrate transporters [8]. So far, more than 21 NPF members have been demonstrated to be able to transport nitrate in Arabidopsis, many of which are able to transport other substrates, such as ABA, GA, JA and glucosinolates [8,13]. Screening 45 out of 53 AtNPF members using modi ed yeast two-hybrid system with ABA, GA…”

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confidence: 99%

“…Three Arabidopsis NPF8 subfamily members, AtNPF8.1/PTR1, AtNPF8.2/PTR5 and AtNPF8.3/PTR2 have been proved to be dipeptide transporters, while AtNPF8.1 and AtNPF8.2 are able to transport JA-Ile simultaneously [34,35]. It is noteworthy that 38 and 17 NPF genes have been characterized at the functional level in Arabidopsis and rice respectively, and more than half of them function on nitrate transport and are distributed on eight NPF subfamilies, and play important roles in nitrate absorption, translocation and utilization [13,31,36,37]. Generally, NPF transporters with a very broad substrate speci city have an important function in plant growth and development, and genome-wide identi cation has been implemented in poplar [38], Medicago truncatula [36], apple [39] and wheat [13], but a few systematic analyses have been conducted for NPF genes in Brassica species.…”

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confidence: 99%

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NPF genes excavation and their expression response to vernalization and nitrogen deficiency in allotetraploid rapeseed

Chao

Zhao

et al. 2021

Preprint

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Background The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF) genes, initially characterized as nitrate or peptide transporters in plants, involve in the transport of a large variety of substrates including amino acids, nitrate, auxin (IAA), jasmonates (JAs), abscisic acid (ABA) and gibberellins (GAs) and glucosinolates. The evolution and expression diversification of genes determine their functional differentiation in polyploid species. Results Among 169 NPF genes excavated in Brassica napus, 97 B. napus NPF (BnaNPF) genes evolved from B. rapa, and 72 BnaNPF genes from B. olereaca. They unevenly distributed on B. napus genome and exhibited obvious synteny with NPF genes in Arabidopsis thaliana, B. rapa and B. olereaca. BnaNPF genes were identified to show diversified expression patterns in 90 different organs or tissues based on transcriptome profile data. Besides, they exhibited complex expression changes in the development process of leaves, silique wall and seeds, which indicated that the expression of BnaNPF genes maybe respond to altered phytohormone and secondary metabolite content through combining with promoter elements enrichment analysis. Furthermore, many BnaNPF genes were detected to response to vernalization with two different patterns and 20 BnaNPF genes responded to nitrate deficiency. Conclusion The evolution of BnaNPF genes and their expression pattern including response to vernalization and nitrogen deficiency were characterized and provide valuable information for further functional characterization in rapeseed.

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Genome‐wide identification of nitrate transporter 1/peptide transporter family (NPF) in cassava (Manihot esculenta) and functional analysis of MeNPF5.4 and MeNPF6.2 in response to nitrogen and salinity stresses in rice

Ji,

Song,

Zou

et al. 2023

Crop Science

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Nitrogen is a major driving force for the improvement of crop yield worldwide,but brings detrimental effects on ecosystems.Therefore, enhancing nitrogen use efficiency (NUE)is vital forsustainableagriculture. The nitrate transporter (NRT/NPF) family associated with nitrogen uptake and utilization isindispensable to the improvement of NUE in crops. As an important food security crop, cassava (Manihot esculenta) produces an acceptable yield in nutrient‐deficient soil. Here we identified and systematically analyzed the NPF gene family in cassava, including phylogenetic relationship, chromosome location, gene duplication, and gene expression in response to different nitrogen supply. The stem is involved in nitrogen transportation and remobilization. Gene expression analysis revealed thatMeNPF5.4 and MeNPF6.2 were specifically expressed in stem, andhave diverse expression in different nitrogen conditions.To facilitate the functional analysis of MeNPF5.4 and MeNPF6.2, we constructed their overexpression (OE) lines in rice. A NO3− flux assayshowed that MeNPF5.4and MeNPF6.2OE lines exhibited a marked decrease NO3− efflux and significant NO3− influxcompared with WT, which suggests that they might have contributed to NUE improvementof rice. Notably, overexpressing MeNPF5.4showed increased grain size, grain number and weight per panicle. More importantly,MeNPF5.4 OE linecontributed to salt tolerance. Nevertheless, anobvious reduction in grain number and weight per panicle was detected in the MeNPF6.2 OE line compared with WT. Strikingly, MeNPF6.2OE line showed higher salt stress tolerance than WT in LN conditions. Taken together, our results demonstrated that MeNPF5.4 can potentially improve the NUE and salt stress tolerance of rice,which reveals valuable breeding targets to improve crop yield and stress tolerance.This article is protected by copyright. All rights reserved

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Phylogeny and gene expression of the complete NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY in Triticum aestivum

Cited by 38 publications

References 78 publications

Physiological and Molecular Traits Associated with Nitrogen Uptake under Limited Nitrogen in Soft Red Winter Wheat

Physiological and Molecular Traits Associated with Nitrogen Uptake under Limited Nitrogen in Soft Red Winter Wheat

NPF genes excavation and their expression response to vernalization and nitrogen deficiency in allotetraploid rapeseed

Genome‐wide identification of nitrate transporter 1/peptide transporter family (NPF) in cassava (Manihot esculenta) and functional analysis of MeNPF5.4 and MeNPF6.2 in response to nitrogen and salinity stresses in rice

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