Callus induction and plant regeneration from lateral shoots of herbaceous bamboo <i>Mniochloa abersend</i>

Zang, Qiaolu; Jiao, Yulian; Guo, Xiaoli; Fei, Zhuge; Yeh, Kai‐Wun; Lin, Xinchun

doi:10.1080/14620316.2016.1232610

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…Establishing a regeneration and genetic transformation system in bamboo is difficult ( Zang et al, 2016 , 2017 ). To examine the function of PvPin1 in regulating flowering, we overexpressed PvPin1 under control of the CaMV 35S promoter in the pCAMBIA1301 vector in Arabidopsis .…”

Section: Resultsmentioning

confidence: 99%

Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice

Zhang

Yang

et al. 2017

Front. Plant Sci.

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Because of the long and unpredictable flowering period in bamboo, the molecular mechanism of bamboo flowering is unclear. Recent study showed that Arabidopsis PIN1-type parvulin 1 (Pin1At) is an important floral activator and regulates floral transition by facilitating the cis/trans isomerization of the phosphorylated Ser/Thr residues preceding proline motifs in suppressor of overexpression of CO 1 (SOC1) and agamous-like 24 (AGL24). Whether bamboo has a Pin1 homolog and whether it works in bamboo flowering are still unknown. In this study, we cloned PvPin1, a homolog of Pin1At, from Phyllostachys violascens (Bambusoideae). Bioinformatics analysis showed that PvPin1 is closely related to Pin1-like proteins in monocots. PvPin1 was widely expressed in all tested bamboo tissues, with the highest expression in young leaf and lowest in floral bud. Moreover, PvPin1 expression was high in leaves before bamboo flowering then declined during flower development. Overexpression of PvPin1 significantly delayed flowering time by downregulating SOC1 and AGL24 expression in Arabidopsis under greenhouse conditions and conferred a significantly late flowering phenotype by upregulating OsMADS56 in rice under field conditions. PvPin1 showed subcellular localization in both the nucleus and cytolemma. The 1500-bp sequence of the PvPin1 promoter was cloned, and cis-acting element prediction showed that ABRE and TGACG-motif elements, which responded to abscisic acid (ABA) and methyl jasmonate (MeJA), respectively, were characteristic of P. violascens in comparison with Arabidopsis. On promoter activity analysis, exogenous ABA and MeJA could significantly inhibit PvPin1 expression. These findings suggested that PvPin1 may be a repressor in flowering, and its delay of flowering time could be regulated by ABA and MeJA in bamboo.

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Section: Resultsmentioning

confidence: 99%

Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice

Zhang

Yang

et al. 2017

Front. Plant Sci.

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“…A propagation system for Garnem rootstock (almond × peach) was conducted and the highest root number was achieved by using ½MS medium with 2.0 mg dm -3 IBA (Erfani et al 2017). In addition, the vigorous roots are also generated in ½MS medium supplemented with 0.5 mg dm -3 IBA (Zang et al 2017). However, high induction rate of adventitious roots could be achieved by using MS media supplemented with 2.0 mg dm -3 IBA in an efficient in vitro tissue culture system for Crocus sativus (Zeybek et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanus

Kang¹,

Zhou²,

Jiang³

et al. 2021

Biol. plant.

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Rapid propagation of plants by tissue culture is of great significance for large-scale production, molecular genetics research, and breeding. Currently, a rapid and high-efficient tissue culture protocol for Euonymus bungeanus is needed. To develop a propagation system for this species, we established a new regeneration system from leaves. Callus formation was induced on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm -3 6-benzylaminopurine (6-BA) and 0.5 mg dm -3 α-naphthalene acetic acid (NAA) and the induction rate almost reached 100 % under red radiation within 21 d. The medium for proliferation of adventitious buds comprised of MS medium with 1.0 mg dm -3 6-BA and 0.5 mg dm -3 NAA, and the induction rate within 20 d nearly reached 100 %. When, the adventitious buds were transferred to the rooting medium containing ½MS, 2.0 mg dm -3 indole-3-butyric acid (IBA), and 0.05 mg dm -3 NAA, adventitious root formation was achieved within 20 d. Collectively, the rapid and high-efficient regeneration system from E. bungeanus leaves was established, providing useful references for effective mass propagation and it could serve as an enabling technology for future genetic engineering.

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“…The best example of somatic embryogenesis in halophytes was reported in S. europaea , where mature embryos were the best type of explant for callus induction and in vitro regeneration, through short treatment with 2,4-D in mature seeds, and callus induction from hypocotyls in MS medium supplemented with 4.55 μmol/L TDZ for 3–4 weeks after germination. The callus differentiated into somatic embryos with shoots at a 27.60% ratio after subculture with indole-3-butyric acid (IBA), Kin and activated charcoal (AC) [ 64 ], as for H. glomeratus where its subculture is crucial for callus proliferation and embryogenic callus formation, as well as a low level of 2,4-D, needed for callus differentiation during this step [ 130 ]. In addition, a relatively low water content in callus plays a key role in somatic embryo formation and is beneficial for plants [ 131 , 132 ].…”

Section: Micropropagation Of Halophyte Plantsmentioning

confidence: 99%

Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review

Custódio

Charles

Magné

et al. 2022

Plants

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Halophytes are plants able to thrive in environments characterized by severe abiotic conditions, including high salinity and high light intensity, drought/flooding, and temperature fluctuations. Several species have ethnomedicinal uses, and some are currently explored as sources of food and cosmetic ingredients. Halophytes are considered important alternative cash crops to be used in sustainable saline production systems, due to their ability to grow in saline conditions where conventional glycophyte crops cannot, such as salt-affected soils and saline irrigation water. In vitro plant tissue culture (PTC) techniques have greatly contributed to industry and agriculture in the last century by exploiting the economic potential of several commercial crop plants. The application of PTC to selected halophyte species can thus contribute for developing innovative production systems and obtaining halophyte-based bioactive products. This work aimed to put together and review for the first time the most relevant information on the application of PTC to halophytes. Several protocols were established for the micropropagation of different species. Various explant types have been used as starting materials (e.g., basal shoots and nodes, cotyledons, epicotyls, inflorescence, internodal segments, leaves, roots, rhizomes, stems, shoot tips, or zygotic embryos), involving different micropropagation techniques (e.g., node culture, direct or indirect shoot neoformation, caulogenesis, somatic embryogenesis, rooting, acclimatization, germplasm conservation and cryopreservation, and callogenesis and cell suspension cultures). In vitro systems were also used to study physiological, biochemical, and molecular processes in halophytes, such as functional and salt-tolerance studies. Thus, the application of PTC to halophytes may be used to improve their controlled multiplication and the selection of desired traits for the in vitro production of plants enriched in nutritional and functional components, as well as for the study of their resistance to salt stress.

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Callus induction and plant regeneration from lateral shoots of herbaceous bamboo Mniochloa abersend

Cited by 8 publications

References 23 publications

Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice

Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanus

Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review

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