Nucleic acid detection using carbon nanoparticles as a fluorescent sensing platform

Li, Hailong; Zhang, Yingwei; Wang, Lei; Tian, Jingqi; Sun, Xuping

doi:10.1039/c0cc04326e

Cited by 283 publications

(167 citation statements)

References 16 publications

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“…Interestingly, many of such materials also exhibit excellent fluorescence quenching and DNA adsorption properties, which have been used in biosensor development in a similar way to GO. For example, the Sun's group and many others have tested a diverse range of materials for DNA adsorption and fluorescence quenching [133][134][135][136][137][138]. We also reported adsorption of DNA by metal oxides [139,140], and nucleotide coordinated nanoparticles [141].…”

Section: Graphene Oxide Like Materialsmentioning

confidence: 85%

Fluorescent sensors using DNA-functionalized graphene oxide

et al. 2014

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In the past few years, graphene oxide (GO) has emerged as a unique platform to develop DNA-based biosensors. This takes advantage of the DNA adsorption and fluorescence quenching property of GO.The adsorbed DNA probes can be desorbed from the GO surface in the presence of target analytes, producing fluorescence signal. In addition to this initial design, many other strategies have been reported including the use of aptamers, molecular beacons, and DNAzymes as probes, label-free detection, using the intrinsic fluorescence of GO and covalently linked DNA probes. The potential applications of DNA-functionalized GO range from environmental monitoring, cell imaging and biomedical diagnosis. In this review, we first summarize the fundamental surface interactions between DNA and GO and the related fluorescence quenching mechanism. Following that, the various sensor design strategies are critically compared. Problems at the current stages are described and a few future directions are also discussed.

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Section: Graphene Oxide Like Materialsmentioning

confidence: 85%

Fluorescent sensors using DNA-functionalized graphene oxide

et al. 2014

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“…Recently, nanosized carbon materials, such as carbon nanotubes, graphene, and graphene oxide have been gradually utilized as competitive acceptors in FRET. That is because of they have super quenching capacity and selfassembly with ssDNA by the π-stacking interaction [18]. Ju designed a platform for the sensing of biomolecules by FRET from QDs to graphene oxide [19].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles

et al. 2014

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We report on a method for simultaneous detection of the pathogens Vibrio parahaemolyticus and Salmonella typhimurium. It is based on dual fluorescence resonance energy transfer (FRET) from green-emitting quantum-dots (gQDs) and red-emitting quantum-dots (rQDs) as donors, and on novel amorphous carbon nanoparticles (CNPs) that act as acceptor. The gQDs were modified with an aptamer (Apt 1) recognizing V. parahaemolyticus, and the rQDs with an aptamer (Apt 2) recognizing S. typhimurium. The fluorescence of both QDs is strongly quenched in the presence of CNPs. However, on addition of the target analytes, the QDsaptamer-target complex is formed and quenching by CNPs is suppressed. The fluorescence of the QDs is linearly proportional to the concentration of the two pathogens in the range from 50 to 10 6 cfu·mL −1 , with detection limits as low as 25 cfu·mL −1 for V. parahaemolyticus, and of 35 cfu·mL −1 for S. typhimurium. The assay was applied to real food samples, and the results were consistent with the results obtained with plate counting methods. We presume that this strategy can be extended to the detection of other pathogenic bacteria and biomolecules by simply substituting the aptamer.

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“…Compared to traditional organic dyes, colloidal semiconductor nanoparticles (also referred to as quantum dots, QDs) possess a series of merits such as broad excitation and size-tunable emission spectra, low-cost, simple synthetic routes, relatively high quantum yields (QYs), and photochemical stability. Due to these properties, semiconductor nanoparticles are widely used as uorescent nanosensors for the detection of metal ions ( [32][33][34] etc. by monitoring the changes in their uorescence intensity.…”

mentioning

confidence: 99%

Dithizone-etched CdTe nanoparticles-based fluorescence sensor for the off–on detection of cadmium ion in aqueous media

Liao

Ding

et al. 2017

RSC Adv.

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In the present study, a new fluorescence probe based on dithizone-etched CdTe nanoparticles was designed for the sensitive and selective detection of cadmium ions in environmental samples via a reversible off-on fluorescence mode. At first, the initial bright fluorescence of L-cysteine-capped CdTe NPs could be effectively quenched in the presence of dithizone (DZ) due to the chemical etching effect, which results from the breaking of Cd-thiol layers by DZ, thus leading to a decrease in the NPs surface passivation. Then, upon the addition of Cd 2+ , the weak fluorescence of the CdTe NPs-DZ system gradually recovered, owing to the occurrence of Cd-thiol passivation layers on the surface of the NPs.Under optimal conditions, a good linear relationship was obtained in the range from 0.4 mM to 15.4 mM for Cd 2+ , with a detection limit of 0.13 mM. In addition, this CdTe NPs-based nanosensor presents remarkable selectivity for Cd 2+ over other metal ions and was successfully applied for the detection of Cd 2+ in real water samples with satisfactory results, demonstrating its potential application for the determination of Cd 2+ in the environment.

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Nucleic acid detection using carbon nanoparticles as a fluorescent sensing platform

Cited by 283 publications

References 16 publications

Fluorescent sensors using DNA-functionalized graphene oxide

Fluorescent sensors using DNA-functionalized graphene oxide

Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles

Dithizone-etched CdTe nanoparticles-based fluorescence sensor for the off–on detection of cadmium ion in aqueous media

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