Evaluation of salinomycin isolated from Streptomyces albus JSY-2 against the ciliate, Ichthyophthirius multifiliis

Abstract

The present study was undertaken to investigate the antiparasitic activity of extracellular products of Streptomyces albus. Bioactivity-guided isolation of chloroform extracts affording a compound showing potent activity. The structure of the compound was elucidated as salinomycin (SAL) by EI-MS, 1H NMR and 13C NMR. In vitro test showed that SAL has potent anti-parasitic efficacy against theronts of Ichthyophthirius multifiliis with 10 min, 1, 2, 3 and 4 h (effective concentration) EC50 (95% confidence intervals) of 2.12 (2.22–2.02), 1.93 (1.98–1.88), 1.42 (1.47–1.37), 1.35 (1.41–1.31) and 1.11 (1.21–1.01) mg L−1. In vitro antiparasitic assays revealed that SAL could be 100% effective against I. multifiliis encysted tomonts at a concentration of 8.0 mg L−1. In vivo test demonstrated that the number of I. multifiliis trophonts on Erythroculter ilishaeformis treated with SAL was markedly lower than that of control group at 10 days after exposed to theronts (P < 0.05). In the control group, 80% mortality was observed owing to heavy I. multifiliis infection at 10 days. On the other hand, only 30.0% mortality was recorded in the group treated with 8.0 mg L−1 SAL. The median lethal dose (LD50) of SAL for E. ilishaeformis was 32.9 mg L−1.

Actinomycetes as a promising candidate bacterial group for the health management of aquaculture systems: A review

“…have been studied and found to kill the theronts and tomonts of Ichthyophthirius multifiliis and Cryptocaryon irritans. 48,49,119,122,141,142 Several studies have been demonstrated in fish and shellfish aquaculture using actinomycetes as antimicrobial sources. 41,59,60,68,99,101,107,111,123,128,131,133,134,137,[143][144][145][196][197][198][199][200] The different species of Actinomycetes used in aquaculture over the years have been summarized in Table 1.…”

“…The anti‐parasitic effects of extracts from different Streptomyces sp. have been studied and found to kill the theronts and tomonts of Ichthyophthirius multifiliis and Cryptocaryon irritans 48,49,119,122,141,142 . Several studies have been demonstrated in fish and shellfish aquaculture using actinomycetes as antimicrobial sources 41,59,60,68,99,101,107,111,123,128,131,133,134,137,143–145,196–200 …”