Evaluation of cationic polymer-coated nanocapsules as ocular drug carriers

Calvo, Pilar; Vila-Jato, J. L.; Alonso, María José

doi:10.1016/s0378-5173(97)00083-5

Cited by 312 publications

(163 citation statements)

References 17 publications

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“…Chitosan-based NC and nanoemulsions (NE) were prepared according to with the protocol developed by Calvo et al [48] with slight modifications introduced in our recent studies [45]. NE were prepared under an identical protocol but without the addition of chitosan and HCl in the aqueous phase.…”

Section: Preparation Of the Nanoformulationsmentioning

confidence: 99%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Highlights Chitosan-coated nanocapsules, but not nanoemulsions, influence the -potential of E.coli  A fixed number of nanocapsules binds and promotes aggregation of bacteria  In silico simulations agree closely with experimental results  Chitosan-coated nanocapsules attenuate the bacterial quorum sensing response § These authors contributed equally to this publication * Author for correspondence Colloids and Surfaces B: Biointerfaces 149 (2017) 358-368 AbstractWe examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ~114-155 nm, polydispersity ~0.127, and -potential ~+50 mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a "stoichiometric" ratio of ~80 NC/bacterium. By SEM it was possible to image the aggregation between NC and bacteria. Moreover, we developed a custom in silico platform to simulate the behavior of particles with diameters of 150 nm and -potential of +50 mV on the bacterial surface. We computed the detailed force interactions between NC-NC and NC-bacteria and found that a maximum number of 145 particles might interact at the bacterial surface. Additionally, we found that the "stoichiometric" ratio of NC and bacteria has a strong influence on the bacterial behavior and influences the quorum sensing response, particularly due to the aggregation driven by NC.

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Section: Preparation Of the Nanoformulationsmentioning

confidence: 99%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…Preparation of acyclovir loaded chitosan nanoparticles Chitosan nanoparticles were prepared based on the ionic gelation of chitosan with sodium tripolyphosphate anions. [15] Chitosan nanoparticles were prepared by ionic gelation of chitosan solution with sodium tripolyphosphate prepared in the presence of Tween 80 as a re-suspending agent to prevent aggregation, at ambient temperature. 350 mg acyclovir and various concentrations of chitosan dissolved in acetic acid in aqueous solution under magnetic stirring at room temperature for 45 min in the presence of Tween 80; 10 mL of sodium tripolyphosphate aqueous solution was added into 10 mL chitosan-acyclovir solution and the mixture was subjected to different sonication times.…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

Formulation and in vivo Evaluation of Acyclovir Loaded Chitosan Nanoparticles for Ocular Delivery

Selvaraj¹,

Niraimathi

2017

IJPSDR

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The present study was aimed to formulate and evaluate chitosan nanoparticles containing acyclovir as potential ophthalmic drug delivery system. The topical application of acyclovir as eye ointment remains a concern for effective management of various ocular viral diseases owing to poor ocular drug bioavailability. The acyclovir loaded chitosan nanoparticles were prepared by ionic gelation of chitosan. Differential scanning calorimetry and fourier transform infra red spectroscopy measurements were carried out on the prepared nanoparticles, pure acyclovir and chitosan polymer. Fifteen different formulations were prepared and evaluated for particle size, Zeta potential, scanning electron microscopy, entrapment and loading capacity and in-vitro drug release profile. All the prepared formulations resulted in nano size in 377.9 to 720.6 nm and displayed spherical shape with zeta potential of +33.2 to +42.8 mV. The encapsulation efficiency and loading capacity were 70.7% -90.9% and 25% -50.8% respectively. The acyclovir loaded chitosan nanoparticles displayed crystallinity than acyclovir. The invitro release profile of acyclovir from the nanoparticles showed a sustained release of the drug over a prolonged period of 24 hours and fit best with Higuchi model with zero order and non-Fickian diffusion was superior phenomenon. The in vivo results reveal that ocular viral infections can be inhibited by the nanoparticles more significantly than the drug in conventional dosage forms. No appreciable difference was observed during 90 days in which nanoparticles were stored at various temperatures. Thus the results suggest that acyclovir loaded chitosan nanoparticle suspension appears promising for effective management of ocular viral infections.

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“…Encapsulation efficiency was determined by an indirect method (Calvo et al, 1997;Cui, Mumper, 2001). Microparticle dispersions containing 250 mg/mL of vitamin E were prepared in phosphate buffer (pH = 7.2) containing 2 mM polysorbate 80.…”

Section: Drug Loading Determinationmentioning

confidence: 99%

A study of the characteristics and in vitro permeation properties of CMC/ chitosan microparticles as a skin delivery system for vitamin E

Alencastre¹,

Bentley²,

Garcia³

et al. 2006

Rev. Bras. Cienc. Farm.

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Evaluation of cationic polymer-coated nanocapsules as ocular drug carriers

Cited by 312 publications

References 17 publications

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Formulation and in vivo Evaluation of Acyclovir Loaded Chitosan Nanoparticles for Ocular Delivery

A study of the characteristics and in vitro permeation properties of CMC/ chitosan microparticles as a skin delivery system for vitamin E

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