Differential transmission of Sri Lankan cassava mosaic virus by three cryptic species of the whitefly Bemisia tabaci complex

Chi, Yuan; Pan, Li‐Long; Bouvaine, Sophie; Fan, Yun-Yun; Liu, Yin-Quan; Liu, Shu‐Sheng; Seal, Susan; Wang, Xiaowei

doi:10.1016/j.virol.2019.11.013

Cited by 36 publications

(31 citation statements)

References 33 publications

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“…Host plants clusters are denoted by black, pink, purple and cyan correspond to the whitefly clusters with the same colours on the X axis. SSA1 (purple), SSA6 (pink) and MED-ASL (cyan) have distinct host profiles on Manihot esculenta (purple), Ocimum gratissimum (pink) and Ipomoea batatas, Cucurbita moschata, Sida acuta (cyan), respectively ◂ Table 4 Predominant Bemisia tabaci and Bemisia Uganda1 associations with plants from which collections were made in 2013 survey Plant associations supported by at least 3 whiteflies were selected from Table 1 and Furthermore, for whiteflies to play an important role in vectoring a plant virus, there is no need for colonisation with the efficiency of transmission dependent on feeding behaviour and whitefly virus specificity (Czosnek et al 2017;Chi et al 2020).…”

Section: Abundance and Host Range Of Whitefly Speciesmentioning

confidence: 99%

“…Members of the B. tabaci cryptic species complex possess distinct biological traits not only in their abundance and invasiveness, but also in their resistance to insecticides, host colonisation range, inducement of phytotoxic disorders and ability to transmit specific begomoviruses (Perry 1985;Brown et al 1995;Jones 2003;Seal et al 2006b;Liu et al 2007;Vyskočilová et al 2019;Chi et al 2020). In the absence of reliable morphological features, mtCO1 barcoding (Frohlich et al 1999;Dinsdale et al 2010), genome-wide SNP markers and whole genome sequencing approaches (Wosula et al 2017;Chen et al 2019;de Moya et al 2019;Elfekih et al 2019;Mugerwa et al 2020) have been used to generate an improved understanding of the systematics within this species complex.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of whitefly (Bemisia spp.) on crop and uncultivated plants in Uganda: implications for the control of this devastating pest species complex in Africa

et al. 2021

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Over the past three decades, highly increased whitefly (Bemisia tabaci) populations have been observed on the staple food crop cassava in eastern Africa and associated with ensuing viral disease pandemics and food insecurity. Increased whitefly numbers have also been observed in other key agricultural crops and weeds. Factors behind the population surges on different crops and their interrelationships are unclear, although in cassava they have been associated with specific populations within the Bemisia tabaci species complex known to infest cassava crops in Africa. This study carried out an in-depth survey to understand the distribution of B. tabaci populations infesting crops and uncultivated plant hosts in Uganda, a centre of origin for this pest complex. Whitefly samples were collected from 59 identified plant species and 25 unidentified weeds in a countrywide survey. Identities of 870 individual adult whiteflies were determined through mitochondrial cytochrome oxidase 1 sequences (651 bp) in the 3′ barcode region used for B. tabaci systematics. Sixteen B. tabaci and five related whitefly putative species were identified based on > 4.0% nucleotide divergence, of which three are proposed as novel B. tabaci putative species and four as novel closely related whitefly species. The most prevalent whiteflies were classified as B. tabaci MED-ASL (30.5% of samples), sub-Saharan Africa 1 (SSA1, 22.7%) and Bemisia Uganda1 (12.1%). These species were also indicated to be the most polyphagous occurring on 33, 40 and 25 identified plant species, respectively. Multiple (≥ 3) whitefly species occurred on specific crops (bean, eggplant, pumpkin and tomato) and weeds (Sida acuta and Ocimum gratissimum). These plants may have increased potential to act as reservoirs for mixed infections of whitefly-vectored viruses. Management of whitefly pest populations in eastern Africa will require an integration of approaches that consider their degree of polyphagy and a climate that enables the continuous presence of crop and uncultivated plant hosts.

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Section: Abundance and Host Range Of Whitefly Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of whitefly (Bemisia spp.) on crop and uncultivated plants in Uganda: implications for the control of this devastating pest species complex in Africa

et al. 2021

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“…According to the phylogenetic analysis, the partial coding sequence of mtCO1 of B. tabaci from Thailand was classified as an Asia II 1 cryptic species. Asia II 1 whiteflies readily transmit SLCMV, whereas Middle East Asia Minor 1 and Mediterranean whiteflies are poor vectors of the virus [41]. Thus, the potential for virus transmission is associated with the virus and whitefly species.…”

Section: Discussionmentioning

confidence: 99%

Survey and Molecular Detection of Sri Lankan Cassava Mosaic Virus in Thailand

Siriwan

Saokham

Hemniam

et al. 2021

Preprint

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Cassava plantations in an area of 458 ha spanning five provinces along the Thailand–Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease severity was generally scored as 2–3. The highest average disease severity was in Sakaeo province (3.7), followed by Buriram (3.6), Prachinburi (2.88), Surin (2.5), and Sisaket (2.4) provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Interestingly, cassava cultivars CMR-89 and Rayong 11 were susceptible to CMD. In approximately 95% of cases, the infection was transmitted by whitefly ( Bemisia tabaci ), which had a high population density in Prachinburi but was sparse in Surin, with the largest populations observed in May and June. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 ( mtCO1 ) gene of whitefly ( Bemisia tabaci ) in Thailand revealed a similarity to the Asia II 1 whitefly gene. Furthermore, the AV1 gene—which encodes the capsid protein—showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.

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“…We thus examined whether the function of BtVAMP2 in virus acquisition as demonstrated in MEAM1 was conserved in Asia II 1. According to a previous study, Asia II 1 whitefly could effectively transmit SLCMV [20]. We performed Co-IP and GST pull-down assays to examine the interaction between BtVAMP2 of Asia II Analyses of the virus quantity in whitefly and the transmission efficiency of TYLCV by whiteflies after feeding with anti-BtVAMP2 pAb.…”

Section: Verification Of Interaction Between Btvamp2 and Slcmv Cpmentioning

confidence: 99%

Bemisia tabaci Vesicle-Associated Membrane Protein 2 Interacts with Begomoviruses and Plays a Role in Virus Acquisition

Fan

Zhong

Zhao

et al. 2021

Cells

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Begomoviruses cause substantial losses to agricultural production, especially in tropical and subtropical regions, and are exclusively transmitted by members of the whitefly Bemisia tabaci species complex. However, the molecular mechanisms underlying the transmission of begomoviruses by their whitefly vector are not clear. In this study, we found that B. tabaci vesicle-associated membrane protein 2 (BtVAMP2) interacts with the coat protein (CP) of tomato yellow leaf curl virus (TYLCV), an emergent begomovirus that seriously impacts tomato production globally. After infection with TYLCV, the transcription of BtVAMP2 was increased. When the BtVAMP2 protein was blocked by feeding with a specific BtVAMP2 antibody, the quantity of TYLCV in B. tabaci whole body was significantly reduced. BtVAMP2 was found to be conserved among the B. tabaci species complex and also interacts with the CP of Sri Lankan cassava mosaic virus (SLCMV). When feeding with BtVAMP2 antibody, the acquisition quantity of SLCMV in whitefly whole body was also decreased significantly. Overall, our results demonstrate that BtVAMP2 interacts with the CP of begomoviruses and promotes their acquisition by whitefly.

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Differential transmission of Sri Lankan cassava mosaic virus by three cryptic species of the whitefly Bemisia tabaci complex

Cited by 36 publications

References 33 publications

Genetic diversity of whitefly (Bemisia spp.) on crop and uncultivated plants in Uganda: implications for the control of this devastating pest species complex in Africa

Genetic diversity of whitefly (Bemisia spp.) on crop and uncultivated plants in Uganda: implications for the control of this devastating pest species complex in Africa

Survey and Molecular Detection of Sri Lankan Cassava Mosaic Virus in Thailand

Bemisia tabaci Vesicle-Associated Membrane Protein 2 Interacts with Begomoviruses and Plays a Role in Virus Acquisition

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