Bemisia tabaci Vesicle-Associated Membrane Protein 2 Interacts with Begomoviruses and Plays a Role in Virus Acquisition

Fan, Yun; Zhong, Yu-Wei; Zhao, Jing; Chi, Yuan; Bouvaine, Sophie; Liu, Shu‐Sheng; Seal, Susan; Wang, Xiao‐Wei

doi:10.3390/cells10071700

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…The vesicle‐associated membrane protein‐associated protein B interacted with the CP of TYLCV, 11 and the collagen protein interacting with the CP of ToLCNDV is associated with viral transmission 12 . The CP of TYLCV and Sri Lankan cassava mosaic virus interacted with the vesicle‐associated membrane protein 2 in B. tabaci and promoted their acquisition 13 . Studying the function of proteins in the B. tabaci MED that interacts with RaMoV CP is the key to explain the relationship between RaMoV and whitefly.…”

Section: Introductionmentioning

confidence: 99%

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Zinc finger protein 330 regulates Ramie mosaic virus infection in the whitefly Bemisia tabaciMED

Peng

Gao

Shi

et al. 2023

Pest Management Science

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BACKGROUND: The whitefly, Bemisia tabaci (Gennadius) is one of the most economically important pests that cause serious damage to agricultural production by transmitting plant pathogenic viruses. Approximately 90% of the virus species transmitted by the whitefly are members of the genus begomovirus. Ramie mosaic virus (RaMoV) is a new bipartite begomovirus that causes severe damage to ramie and several other economic crops in China. In previous studies, we have demonstrated that RaMoV had no obvious direct or indirect effects on B. tabaci. However, whether B. tabaci affects RaMoV infection and the molecular mechanisms of their interaction remain unclear.RESULTS: Here, we identified a zinc finger protein 330 (ZNF330) in B. tabaci MED interacted with the coat protein (CP) of RaMoV by the yeast two-hybrid assay. Then the interaction between ZNF330 and RaMoV CP was further verified by glutathione Stransferase (GST) pull-down assay. The expression of ZNF330 gene was continuously induced after RaMoV infection. ZNF330 negatively regulated RaMoV replication in the B. tabaci MED. Furthermore, the longevity and fecundity of RaMoV-infected female adults were significantly decreased after silencing of ZNF330. CONCLUSIONS: Our results indicated that the ZNF330 protein was involved in the negative regulation of RaMoV replication in the B. tabaci MED. High viral accumulation caused by ZNF330 silencing is detrimental to fecundity and longevity of the B. tabaci MED. These findings provided a new insight into identifying the binding partners in whitefly with viral CP and fully understanding the complex interactions between begomoviruses and their whitefly vector.

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Section: Introductionmentioning

confidence: 99%

“…12 The CP of TYLCV and Sri Lankan cassava mosaic virus interacted with the vesicle-associated membrane protein 2 in B. tabaci and promoted their acquisition. 13 Studying the function of proteins in the B. tabaci MED that interacts with RaMoV CP is the key to explain the relationship between RaMoV and whitefly.…”

Section: Introductionmentioning

confidence: 99%

Zinc finger protein 330 regulates Ramie mosaic virus infection in the whitefly Bemisia tabaciMED

Peng

Gao

Shi

et al. 2023

Pest Management Science

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Insights into the multifunctional roles of geminivirus-encoded proteins in pathogenesis

et al. 2022

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Characterization of transposable elements within the Bemisia tabaci species complex

et al. 2022

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Background Whiteflies are agricultural pests that cause negative impacts globally to crop yields resulting at times in severe economic losses and food insecurity. The Bemisia tabaci whitefly species complex is the most damaging in terms of its broad crop host range and its ability to serve as vector for over 400 plant viruses. Genomes of whiteflies belonging to this species complex have provided valuable genomic data; however, transposable elements (TEs) within these genomes remain unexplored. This study provides the first accurate characterization of TE content within the B. tabaci species complex. Results This study identified that an average of 40.61% of the genomes of three whitefly species (MEAM1, MEDQ, and SSA-ECA) consists of TEs. The majority of the TEs identified were DNA transposons (22.85% average) while SINEs (0.14% average) were the least represented. This study also compared the TE content of the three whitefly genomes with three other hemipteran genomes and found significantly more DNA transposons and less LINEs in the whitefly genomes. A total of 63 TE superfamilies were identified to be present across the three whitefly species (39 DNA transposons, six LTR, 16 LINE, and two SINE). The sequences of the identified TEs were clustered which generated 5766 TE clusters. A total of 2707 clusters were identified as uniquely found within the whitefly genomes while none of the generated clusters were from both whitefly and non-whitefly TE sequences. This study is the first to characterize TEs found within different B. tabaci species and has created a standardized annotation workflow that could be used to analyze future whitefly genomes. Conclusion This study is the first to characterize the landscape of TEs within the B. tabaci whitefly species complex. The characterization of these elements within the three whitefly genomes shows that TEs occupy significant portions of B. tabaci genomes, with DNA transposons representing the vast majority. This study also identified TE superfamilies and clusters of TE sequences of potential interest, providing essential information, and a framework for future TE studies within this species complex.

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Bemisia tabaci Vesicle-Associated Membrane Protein 2 Interacts with Begomoviruses and Plays a Role in Virus Acquisition

Cited by 4 publications

References 42 publications

Zinc finger protein 330 regulates Ramie mosaic virus infection in the whitefly Bemisia tabaciMED

Zinc finger protein 330 regulates Ramie mosaic virus infection in the whitefly Bemisia tabaciMED

Insights into the multifunctional roles of geminivirus-encoded proteins in pathogenesis

Characterization of transposable elements within the Bemisia tabaci species complex

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