Timeline and geographical distribution of Helicoverpa armigera (Hübner) (Lepidoptera, Noctuidae: Heliothinae) in Brazil

Sosa–Gómez, Daniel Ricardo; Specht, Alexandre; Paula‐Moraes, Silvana V.; Lopes-Lima, Adilson; Yano, Silvia Akimi Cavaguchi; Micheli, Adriana; Morais, E. G. F. de; Gallo, Paulo Rogério; Pereira, Paulo R.V.S.; Salvadori, J. R.; Botton, Marcos; Zenker, Maurício M.; Azevedo-Filho, Wilson Sampaio de

doi:10.1016/j.rbe.2015.09.008

Cited by 66 publications

(58 citation statements)

References 16 publications

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“…In Rio Grande do Sul, H . armigera was not detected in the maize ears (Fig 1), even though this species was reported as present in southern Brazil from at least 2011 [7]. In Planaltina, DF (central Brazil), the proportion of H .…”

Section: Discussionmentioning

confidence: 99%

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Battle in the New World: Helicoverpa armigera versus Helicoverpa zea (Lepidoptera: Noctuidae)

et al. 2016

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The corn earworm Helicoverpa zea (Boddie) and the old world bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) are allopatric species and occur in important agricultural crops. In maize, both species tend to infest the ear. The introduction of H. armigera in Brazil has created a new scenario, where these Helicoverpa species might cohabit and interact with one another, affecting the prevalence of each species in the agroecosystem, integrated pest management, and insect resistance management. In this study, larval occurrence and proportion of these species in maize was assessed in three regions of Brazil during three crop seasons. Interaction between the species was evaluated in interspecific and intraspecific scenarios under laboratory and field conditions. Helicoverpa zea was predominant in Rio Grande do Sul and the Planaltina, DF (central Brazil). In western Bahia, H. zea was predominant in the first collection, but approximately equal in number to H armigera in the second crop season. Both species exhibit high cannibalism/predation rates, and larval size was the primary factor for larval survival in the interaction studies. Larva of H. zea had higher survival when interacting with H. armigera, indicating that H. zea has an advantage in intraguild interactions with H. armigera in maize. Overall, the results from this study indicate that maize might play a role as a source of infestation or a sink of insecticide or Bt protein unselected H. armigera populations, depending on the H. zea:H. armigera intraguild competition and adult movement in the landscape.

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Section: Discussionmentioning

confidence: 99%

“…Indeed, H . armigera was present in Brazil at least since 2008 [7], and the potential for it to spread throughout North and South America [8] has been confirmed by reports of its occurrence in other countries, such as Argentina [9], and in the U.S on 17 June 2015, where three H . armigera moths were collected in Florida [10].…”

Section: Introductionmentioning

confidence: 97%

Battle in the New World: Helicoverpa armigera versus Helicoverpa zea (Lepidoptera: Noctuidae)

et al. 2016

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“…Although the number of insect taxonomists in Brazil has increased in the last decades (Rafael, Melo, Carvalho, Casari, & Constantino, ), most species inventories/assessments are restricted to areas close to universities and research facilities and/or to a particular taxon like order or family (Lewinsohn, Freitas, & Prado, ). The crop damage caused by a caterpillar species recently introduced in Brazil (Sosa‐Gomez et al, ) has prompted a national‐scale monitoring program funded by the Ministry of Agriculture, and a network of automatic light traps was established in 12 sampling areas throughout Brazil with the aim of recording abundance of Helicoverpa armigera (A. Specht, 2015, personal communication). The trap used in this monitoring program attracts a variety of insects (Kato et al, ), which are killed and stored in containers filled with ethanol, offering a great opportunity of using the metabarcoding approach to noninvasively assess insect diversity in a large geographical scale.…”

Section: Introductionmentioning

confidence: 99%

Assessing insect biodiversity with automatic light traps in Brazil: Pearls and pitfalls of metabarcoding samples in preservative ethanol

Zenker

Specht

Fonseca

2020

Ecology and Evolution

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Automated species identification based on data produced with metabarcoding offers an alternative for assessing biodiversity of bulk insect samples obtained with traps. We used a standard two‐step PCR approach to amplify a 313 bp fragment of the barcoding region of the mitochondrial COI gene. The PCR products were sequenced on an Illumina MiSeq platform, and the OTUs production and taxonomic identifications were performed with a customized pipeline and database. The DNA used in the PCR procedures was extracted directly from the preservative ethanol of bulk insect samples obtained with automatic light traps in 12 sampling areas located in different biomes of Brazil, during wet and dry seasons. Agricultural field and forest edge habitats were collected for all sampling areas. A total of 119 insect OTUs and nine additional OTUs assigned to other arthropod taxa were obtained at a ≥97% sequence similarity level. The alpha and beta diversity analyses comparing biomes, habitats, and seasons were mostly inconclusive, except for a significant difference in beta diversity between biomes. In this study, we were able to metabarcode and HTS adult insects from their preservative medium. Notwithstanding, our results underrepresent the true magnitude of insect diversity expected from samples obtained with automatic light traps in Brazil. Although biological and technical factors might have impacted our results, measures to optimize and standardize eDNA HTS should be in place to improve taxonomic coverage of samples of unknown diversity and stored in suboptimal conditions, which is the case of most eDNA samples.

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“…armigera prevalent throughout much of the Old World, including Europe, Asia, Africa, and Australasia [1, 2]. Helicoverpa armigera was first discovered in the New World in Brazil in 2012/2013, and it has since spread to much of South America and the Caribbean, including Puerto Rico [5, 6, 7, 8]. Although not yet present in the continental U.S., three individuals were captured in Florida in 2015 suggesting that establishment is imminent [9, 10].…”

Section: Introductionmentioning

confidence: 99%

A droplet digital PCR (ddPCR) assay to detect Helicoverpa armigera (Lepidoptera: Noctuidae) in bulk trap samples

et al. 2017

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Moths in the genus Helicoverpa are some of the most important agricultural pests in the world. Two species, H. armigera (Hübner) and H. zea (Boddie), cause the majority of damage to crops and millions of dollars are spent annually on control of these pests. The recent introduction of H. armigera into the New World has prompted extensive survey efforts for this species in the United States. Surveys are conducted using bucket traps baited with H. armigera pheromone, and, because the same pheromone compounds attract both species, these traps often capture large numbers of the native H. zea. Adult H. armigera and H. zea are very similar and can only be separated morphologically by minor differences in the genitalia. Thus, a time consuming genitalic dissection by a trained specialist is necessary to reliably identify either species, and every specimen must be dissected. Several molecular methods are available for differentiating and identifying H. armigera and H. zea, including two recently developed rapid protocols using real-time PCR. However, none of the published methods are capable of screening specimens in large batches. Here we detail a droplet digital PCR (ddPCR) assay that is capable of detecting a single H. armigera in a background of up to 999 H. zea. The assay has been tested using bulk extractions of 1,000 legs from actual trap samples and is effective even when using poor quality samples. This study provides an efficient, rapid, reproducible, and scalable method for processing H. armigera survey trap samples in the U.S. and demonstrates the potential for applying ddPCR technology to screen and diagnose invasive species.

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Timeline and geographical distribution of Helicoverpa armigera (Hübner) (Lepidoptera, Noctuidae: Heliothinae) in Brazil

Cited by 66 publications

References 16 publications

Battle in the New World: Helicoverpa armigera versus Helicoverpa zea (Lepidoptera: Noctuidae)

Battle in the New World: Helicoverpa armigera versus Helicoverpa zea (Lepidoptera: Noctuidae)

Assessing insect biodiversity with automatic light traps in Brazil: Pearls and pitfalls of metabarcoding samples in preservative ethanol

A droplet digital PCR (ddPCR) assay to detect Helicoverpa armigera (Lepidoptera: Noctuidae) in bulk trap samples

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