Effects of anti-inflammatory drugs in primary kidney cell culture of a freshwater fish

Ribas, João Luiz Coelho; Silva, Cesar A. da; Andrade, Lucas de Melo; Galvan, Gabrieli Limberger; Cestari, Marta Margarete; Trindade, Edvaldo S.; Zampronio, Aleksander Roberto; Assis, Helena C. Silva de

doi:10.1016/j.fsi.2014.07.009

Cited by 40 publications

(24 citation statements)

References 45 publications

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“…In the catfish macrophage preparations, the NO inhibitor L-NMMA inhibited the LPS-induced NO production to the basal level, as has been reported in H. malabaricus (Ribas et al, 2014). L-NMMA did not inhibit basal nitrite level, indicating that the basal NO level may be due to constitutive expression of NOS and LPS stimulated only the inducible form of NOS.…”

Section: No Productionsupporting

confidence: 70%

“…The optimal LPS concentration required for NO production in macrophages isolated from the trout was in the range of 1-50 µg/mL (Fierro-Castro et al, 2012). However in Hoplias malabaricus, LPS at 1 ng/mL stimulated NO production significantly in the anterior kidney monocytes/macrophages (Ribas et al, 2014). In the common carp, dose-related differences were reported in tissues; LPS at 0.1µg/ mL stimulated significantly NO release in head kidney monocytes/macrophages but a significant up regulation in neutrophils was obtained at LPS concentrations at and above 10 µg/mL (Pijanowski et al, 2015).…”

Section: No Productionmentioning

confidence: 91%

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In Vitro Effects of Lipopolysaccharide and Stress Hormones on Phagocytosis and Nitric Oxide Production by Enriched Head Kidney Macrophage Cultures in the Catfish

Kumar

Joy

2018

Proceedings of the Indian National Science Academy

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Head kidney (HK) is the embryonic pronephric kidney retained in adult teleosts and contains the adrenal homologues (interrenal and chromaffin cells) and hemopoietic tissue. The adrenal homologues secrete the stress hormones glucorticoids (cortisol) and catecholamines, and the hemopoietic tissue is a major source of monocytes/macrophages and neutrophils that serve as the first line of defence against invading pathogens. In the present study, head kidney macrophage-enriched preparations of Heteropneustes fossilis were used to demonstrate the dynamics of phagocytosis and nitric oxide (NO) production in the presence of lipopolysaccharide (LPS; bacterial toxin), the synthetic glucocorticoid dexamethasone and/or catecholamines. The incubation of enriched macrophage cultures with LPS for 6 h at 20 o C stimulated phagocytosis of yeast cells and fluorescent latex beads. On the other hand, the cortisol agonist dexamethasone (10 nM) inhibited phagocytosis of yeast cells and latex beads under similar conditions. LPS stimulated inducible NO synthase (iNOS)-like expression in the macrophage cultures, which was inhibited by dexamethasone in co-incubations. Complementary to the iNOS expression, LPS stimulated NO production (nitrite level), which was inhibited by the NO synthase inhibitor L-NMMA. Dexamethasone inhibited basal as well as the LPS-induced stimulation of NO. The catecholamines epinephrine and norepinephrine did not alter the basal NO level but inhibited the LPS-induced stimulation of NO. Dopamine stimulated NO production only at a higher concentration. The results provide evidence for the existence of an endocrine-immune interaction at the level of the head kidney to modulate macrophage activity and immune functions in the catfish.

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Section: No Productionsupporting

confidence: 70%

Section: No Productionmentioning

confidence: 91%

In Vitro Effects of Lipopolysaccharide and Stress Hormones on Phagocytosis and Nitric Oxide Production by Enriched Head Kidney Macrophage Cultures in the Catfish

Kumar

Joy

2018

Proceedings of the Indian National Science Academy

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“…They also demonstrated the use of a continuous Percoll density gradient resulted in only 50% of a maximum macrophages enrichment (Elkamel et al 2003). Furthermore, prior incubation with Percoll substantially decreases the percentage of adherent cells and cell viability (Wakefield et al 1982;Ribas et al 2014). Therefore, the method we reported here for isolation primary macrophages avoiding the usage of Percoll could make our cell extraction steps more concise.…”

Section: Discussionmentioning

confidence: 90%

“…Over the years, carp head kidney primary macrophages have been cultured essentially followed the method described for goldfish and trout (Tassakka and Sakai 2003;Joerink et al 2006), by which primary macrophages are usually isolated using discontinuous Percoll density gradient. However, several previous studies indicate that reagent Percoll is cytotoxic and prior incubation with it substantially decreases the percentage of adherent cells, and even reduces cell viability (Wakefield et al 1982;Ribas et al 2014). Therefore, to improve the method for primary macrophage isolation, there is a need to avoid Percoll usage in the cell isolation process.…”

Section: Introductionmentioning

confidence: 99%

The primary culture of carp (Cyprinus carpio) macrophages and the verification of its phagocytosis activity

Qiu

Liu

Chen

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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This study establishes the primary culture method for red carp (Cyprinus carpio) macrophages in vitro and lays the foundation for further research in the fish immune system. The healthy adult red carp was chosen, and mechanical separation and cell adherent culture methods were used to isolate the primary macrophages. Compared to the traditional method of Percoll discontinuous density gradient isolation, the protocol we reported here makes cell isolation steps more concise and obtains more healthy cells with high macrophage purity. The cells were uniform in size with a clearly visible nucleus. Trypan blue staining and non-radioactive cell proliferation assay were used to detect the cell survival rate. Further, we provide optimum culture conditions which include cell density (1 × 10(7) cells/mL), culture medium (Leibovitz's L-15), pH (7.2-7.4), temperature (26°C), and adherent time (24 h). Macrophages have been identified by nonspecific esterase and Wright-Giemsa staining and have shown to grow very well. In addition, the macrophages have a very strong bactericidal activity against three kinds of bacteria, further verifying good growth conditions and proper function.

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“…Nonetheless, it is expected that higher concentrations can be found in hospitals wastewaters although there are no reports of these concentrations in literature. Furthermore, water contaminated with pharmaceuticals such as acetaminophen can cause hepatic damage in humans [7,8] and alter the equilibrium of aquatic ecosystems due to its toxicity [9,10]. As a result, there is a need to develop efficient technologies to remove emerging contaminants from industrial wastewater and water bodies.…”

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confidence: 99%

Photocatalytic Degradation of Commercial Acetaminophen: Evaluation, Modeling, and Scaling-Up of Photoreactors

et al. 2018

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Abstract:In this work, the performance of a pilot-scale solar CPC reactor was evaluated for the degradation of commercial acetaminophen, using TiO 2 P25 as a catalyst. The statistical Taguchi's method was used to estimate the combination of initial pH and catalyst load while tackling the variability of the solar radiation intensity under tropical weather conditions through the estimation of the signal-to-noise ratios (S/N) of the controllable variables. Moreover, a kinetic law that included the explicit dependence on the local volumetric rate of photon absorption (LVRPA) was used. The radiant field was estimated by joining the Six Flux Model (SFM) with a solar emission model based on clarity index (K C ), whereas the mass balance was coupled to the hydrodynamic equations, corresponding to the turbulent regime. For scaling-up purposes, the ratio of the total area-to-total-pollutant volume (A T /V T ) was varied for observing the effect of this parameter on the overall plant performance. The Taguchi's experimental design results showed that the best combination of initial pH and catalyst load was 9 and 0.6 g L −1 , respectively. Also, full-scale plants would require far fewer ratios of A T /V T than for pilot or intermediate-scale ones. This information may be beneficial for reducing assembling costs of photocatalytic reactors scaling-up.

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Effects of anti-inflammatory drugs in primary kidney cell culture of a freshwater fish

Cited by 40 publications

References 45 publications

In Vitro Effects of Lipopolysaccharide and Stress Hormones on Phagocytosis and Nitric Oxide Production by Enriched Head Kidney Macrophage Cultures in the Catfish

In Vitro Effects of Lipopolysaccharide and Stress Hormones on Phagocytosis and Nitric Oxide Production by Enriched Head Kidney Macrophage Cultures in the Catfish

The primary culture of carp (Cyprinus carpio) macrophages and the verification of its phagocytosis activity

Photocatalytic Degradation of Commercial Acetaminophen: Evaluation, Modeling, and Scaling-Up of Photoreactors

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