Neuropathologic damage induced by radiofrequency ablation at different temperatures

Yu, Dong; Chen, Ying; Yao, Baoguo; Song, Peng; Xu, Ruiting; Li, Rui; Liu, Ping; Tong, Xin; Ma, Linwei; Yu, Jianjun; Su, Li

doi:10.1016/j.clinsp.2022.100033

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…The sodium channel β3 subunit (SCN3B) is an ion channel gene that is upregulated in the dorsal root ganglion during nerve injury, suggesting neuropathic injury (73). Stimulation of peripheral nerves in diabetic peripheral neuropathy (DPN) patients was demonstrated to activate cognitive-related areas of the brain, such as the temporal lobe and hippocampus (74).…”

Section: Discussionmentioning

confidence: 99%

Potential mechanism of the Shunaoxin pill for preventing cognitive impairment in type 2 diabetes mellitus

Guo

Luo²,

Kang³

2022

Front. Neurol.

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ObjectiveThis study aims to analyze the efficacy and mechanism of action of the Shunaoxin pill in preventing cognitive impairment in diabetic patients using network pharmacology.MethodsThe main active compounds of the Shunaoxin pills and their action targets were identified via the TCMSP and Batman-TCM databases. The GEO database was used to identify the genes in type 2 diabetic individuals associated with cognitive impairment. Subsequently, a common target protein-protein interaction (PPI) network was constructed using the STRING database, and targets associated with diabetes and cognitive impairment were screened by performing a topological analysis of the PPI network. The AutoDock Vina software was used for molecular docking to evaluate the reliability of the bioinformatic analysis predictions and validate the interactions between the active ingredients of the Shunaoxin pill and proteins associated with diabetes and cognitive impairment.ResultsBased on the TCMSP and Batman-Tcm platform, 48 active ingredients of the Shunaoxin pill were identified, corresponding to 222 potential action targets. Further analysis revealed that 18 active components of the Shunaoxin pill might contribute to cognitive impairment in type 2 diabetic patients. Molecular docking simulations demonstrated that the active ingredients of the Shunaoxin pill (hexadecanoic acid, stigmasterol, beta-sitosterol, and angelicin) targeted four core proteins: OPRK1, GABRA5, GABRP, and SCN3B.ConclusionActive ingredients of the Shunaoxin pill may alleviate cognitive impairment in diabetic patients by targeting the proteins OPRK1, GABRA5, GABRP, and SCN3B.

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Section: Discussionmentioning

confidence: 99%

Potential mechanism of the Shunaoxin pill for preventing cognitive impairment in type 2 diabetes mellitus

Guo

Luo²,

Kang³

2022

Front. Neurol.

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Imaging of Peripheral Nerves: AJR Expert Panel Narrative Review

Nwawka,

Adriaensen,

Andreisek

et al. 2024

American Journal of Roentgenology

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Development of an ultrasound-guided radiofrequency ablation technique in the equine cadaveric distal limb: histological findings and potential for treating chronic lameness

Amari,

Rabbogliatti,

Ravasio

et al. 2024

Front. Vet. Sci.

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IntroductionRadiofrequency (RF) relieves chronic pain in humans, but it is unexplored in horses affected by chronic lameness. This study aims to describe the technique and the histological effects of ultrasound (US)-guided radiofrequency ablation (RFA) of palmar digital nerves (PDNs) in horse’s fetlock and pastern, ex vivo.MethodsAfter assessing the US anatomy of lateral and medial PDNs in fetlock and pastern in vivo (n = 10 horses; 20 forelimbs), US-guided RFA was performed on these sites in cadaveric forelimbs (n = 10) applying four different settings with increasing invasiveness (n = 40 total treatments): 60°C, 6 min (GROUP LOW); 70°C, 4 min (GROUP MEDIUM); 90°C, 2 min (GROUP HIGH); 80°C, 8 min (GROUP VERY HIGH). Needle-tip-to-nerve proximity was assessed with US and methylene blue, injected through the port of the RF needle. Nerves were collected for microscopical assessment.ResultsTransverse palmaro-lateral and palmaro-medial US images of fetlock and pastern detected PDNs consistently, close to the palmar digital artery. With in-plane US technique, RFA was performed at target in 31/40 cases, with significantly higher number of failures in fetlock (p = 0.008). PDNs histology identified thermal injury/coagulation with axonal degeneration and collagen homogenation. Nuclear smearing of arterial leyomyocytes was also observed. Nerve coagulation was significantly associated with treatment (p = 0.03) and needle-tip-to-nerve proximity (US distance: p = 0.009; blue distance: p = 0.04).DiscussionThe PDNs were easily visualized and reached with the RF needle by US in-plane-guided technique. RFA produced axonal thermal damage and intensity-related coagulation effectiveness. To ensure effective nerve coagulation, it is crucial that the needle is accurately positioned in close proximity to the target nerve. Based on the histopathological findings, HIGH and VERY HIGH RFA treatments might be worth of being tested in vivo in clinical studies aimed at treating chronic lameness of the distal forelimb in horses.

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Neuropathologic damage induced by radiofrequency ablation at different temperatures

Cited by 3 publications

References 22 publications

Potential mechanism of the Shunaoxin pill for preventing cognitive impairment in type 2 diabetes mellitus

Potential mechanism of the Shunaoxin pill for preventing cognitive impairment in type 2 diabetes mellitus

Imaging of Peripheral Nerves: AJR Expert Panel Narrative Review

Development of an ultrasound-guided radiofrequency ablation technique in the equine cadaveric distal limb: histological findings and potential for treating chronic lameness

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