In vitro permeability enhancement of curcumin across Caco-2 cells monolayers using electrospun xanthan-chitosan nanofibers

Faralli, Adele; Shekarforoush, Elhamalsadat; Ajalloueian, Fatemeh; Mendes, Ana Carina Loureiro; Chronakis, Ioannis S.

doi:10.1016/j.carbpol.2018.10.073

Cited by 74 publications

(39 citation statements)

References 56 publications

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“…In vitro studies have demonstrated that curcumin represents a potential compound to restore disrupted intestinal permeability. Indeed, in CaCo2 cells, curcumin is able to attenuate the disruption of intestinal epithelial barrier function, counteracting LPS-induced IL-1β secretion and preventing tight junction protein disruption [113,114]. Furthermore, curcumin was also able to decrease p38 MAPK activation, induced by IL-1β, and the subsequent raise in the phosphorylation of tight junction proteins and resulting disruption of their normal arrangement [114].…”

Section: Curcumin Acts On Intestinal Barrier Functionmentioning

confidence: 97%

Interaction between Gut Microbiota and Curcumin: A New Key of Understanding for the Health Effects of Curcumin

2020

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Curcumin, a lipophilic polyphenol contained in the rhizome of Curcuma longa (turmeric), has been used for centuries in traditional Asian medicine, and nowadays it is widely used in food as dietary spice worldwide. It has received considerable attention for its pharmacological activities, which appear to act primarily through anti-inflammatory and antioxidant mechanisms. For this reason, it has been proposed as a tool for the management of many diseases, among which are gastrointestinal and neurological diseases, diabetes, and several types of cancer. However, the pharmacology of curcumin remains to be elucidated; indeed, a discrepancy exists between the well-documented in vitro and in vivo activities of curcumin and its poor bioavailability and chemical instability that should limit any therapeutic effect. Recently, it has been hypothesized that curcumin could exert direct regulative effects primarily in the gastrointestinal tract, where high concentrations of this polyphenol have been detected after oral administration. Consequently, it might be hypothesized that curcumin directly exerts its regulatory effects on the gut microbiota, thus explaining the paradox between its low systemic bioavailability and its wide pharmacological activities. It is well known that the microbiota has several important roles in human physiology, and its composition can be influenced by a multitude of environmental and lifestyle factors. Accordingly, any perturbations in gut microbiome profile or dysbiosis can have a key role in human disease progression. Interestingly, curcumin and its metabolites have been shown to influence the microbiota. It is worth noting that from the interaction between curcumin and microbiota two different phenomena arise: the regulation of intestinal microflora by curcumin and the biotransformation of curcumin by gut microbiota, both of them potentially crucial for curcumin activity. This review summarizes the most recent studies on this topic, highlighting the strong connection between curcumin and gut microbiota, with the final aim of adding new insight into the potential mechanisms by which curcumin exerts its effects.

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Section: Curcumin Acts On Intestinal Barrier Functionmentioning

confidence: 97%

Interaction between Gut Microbiota and Curcumin: A New Key of Understanding for the Health Effects of Curcumin

2020

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“…Direct interactions between the fibers and the monolayer induced changes in the tight junctions, and thus, an increase in the permeation of insulin at local hot spots on the epithelial barrier was observed. Similarly, a 3.4-fold increase of curcumin permeability across Caco-2 cells was detected when the bioactive was encapsulated within xanthan-chitosan nanofibers, in comparison with free-curcumin [39].…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, electrospun xanthan-chitosan nanofibers loaded with curcumin, as a model hydrophobic bioactive, were incubated with Caco-2 cells and the transepithelial transport and permeability properties across cell monolayers were assessed. A 3.4-fold increase of curcumin permeability was detected in the presence of xanthan-chitosan nanofibers, in comparison with free-curcumin [38,39]. Moreover, electrospun xanthan nanofibers developed from a solution of xanthan dissolved in formic acid, remained intact and morphologically stable over a wide pH range in saline buffers [40].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Transepithelial Permeation of Gallic Acid and (−)-Epigallocatechin Gallate across Human Intestinal Caco-2 Cells Using Electrospun Xanthan Nanofibers

et al. 2019

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Electrospun xanthan polysaccharide nanofibers (X) were developed as an encapsulation and delivery system of the poorly absorbed polyphenol compounds, gallic acid (GA) and (−)-epigallocatechin gallate (EGCG). Scanning electron microscopy was used to characterize the electrospun nanofibers, and controlled release studies were performed at pH 6.5 and 7.4 in saline buffer, suggesting that the release of polyphenols from xanthan nanofibers follows a non Fickian mechanism. Furthermore, the X-GA and X-EGCG nanofibers were incubated with Caco-2 cells, and the cell viability, transepithelial transport, and permeability properties across cell monolayers were investigated. An increase of GA and EGCG permeability was observed when the polyphenols were loaded into xanthan nanofibers, compared to the free compounds. The observed in vitro permeability enhancement of GA and EGCG was induced by the presence of the polysaccharide nanofibers, which successfully inhibited efflux transporters, as well as by tight junctions opening.

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“…The research also reported that after 24 h of eNFs’s incubation with Caco-2 cells monolayers, a cell viability of ~80% and an increased in vitro transepithelial permeability of curcumin without jeopardizing cellular viability were exhibited. At the same time, a 3.4-fold growth of curcumin permeation when the polyphenol was incorporated into XG-CS eNFS was found, when compared to the free curcumin, a phenomenon that can be explained by contact interactions between the Caco-2 cells and eNFs, which trigger the opening of the tight junctures [ 190 ].…”

Section: Electrospun Nanofibers In Wound Healingmentioning

confidence: 99%

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

et al. 2020

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Currently, despite the thoroughgoing scientific research carried out in the area of wound healing management, the treatment of skin injuries, regardless of etiology remains a big provocation for health care professionals. An optimal wound dressing should be nontoxic, non-adherent, non-allergenic, should also maintain a humid medium at the wound interfacing, and be easily removed without trauma. For the development of functional and bioactive dressings, they must meet different conditions such as: The ability to remove excess exudates, to allow gaseous interchange, to behave as a barrier to microbes and to external physical or chemical aggressions, and at the same time to have the capacity of promoting the process of healing by stimulating other intricate processes such as differentiation, cell adhesion, and proliferation. Over the past several years, various types of wound dressings including hydrogels, hydrocolloids, films, foams, sponges, and micro/nanofibers have been formulated, and among them, the electrospun nanofibrous mats received an increased interest from researchers due to the numerous advantages and their intrinsic properties. The drug-embedded nanofibers are the potential candidates for wound dressing application by virtue of: Superior surface area-to volume ratio, enormous porosity (can allow oxy-permeability) or reticular nano-porosity (can inhibit the microorganisms’adhesion), structural similitude to the skin extracellular matrix, and progressive electrospinning methodology, which promotes a prolonged drug release. The reason that we chose to review the formulation of electrospun nanofibers based on polysaccharides as dressings useful in wound healing was based on the ever-growing research in this field, research that highlighted many advantages of the nanofibrillary network, but also a marked versatility in terms of numerous active substances that can be incorporated for rapid and infection-free tissue regeneration. In this review, we have extensively discussed the recent advancements performed on electrospun nanofibers (eNFs) formulation methodology as wound dressings, and we focused as well on the entrapment of different active biomolecules that have been incorporated on polysaccharides-based nanofibers, highlighting those bioagents capable of improving the healing process. In addition, in vivo tests performed to support their increased efficacy were also listed, and the advantages of the polysaccharide nanofiber-based wound dressings compared to the traditional ones were emphasized.

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In vitro permeability enhancement of curcumin across Caco-2 cells monolayers using electrospun xanthan-chitosan nanofibers

Cited by 74 publications

References 56 publications

Interaction between Gut Microbiota and Curcumin: A New Key of Understanding for the Health Effects of Curcumin

Interaction between Gut Microbiota and Curcumin: A New Key of Understanding for the Health Effects of Curcumin

Enhanced Transepithelial Permeation of Gallic Acid and (−)-Epigallocatechin Gallate across Human Intestinal Caco-2 Cells Using Electrospun Xanthan Nanofibers

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

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